Purification of Cowdria ruminantium organisms for use in genome analysis by pulsed-field gel electrophoresis.

Abstract:

:Cowdria ruminantium is an obligate intracellular rickettsial pathogen which is responsible for a tick-borne disease of domestic and wild ruminants called heartwater or cowdriosis. Although several genes have been cloned and partially sequenced, the genome size, gross structure, and organization of the C. ruminantium genome is unknown. Genome analysis of the organism has been hindered because it is difficult to obtain C. ruminantium DNA free from contaminating host cell DNA, and this probably accounts for the lack of genome size data for this organism. In this study we investigated several methods for purifying C. ruminantium from bovine cellular contaminants and organisms of a relatively high purity were obtained. These were used to prepare Cowdria DNA which was analyzed by pulsed-field gel electrophoresis (PFGE) and which revealed a genome approximately 1900 kbp in length plus an additional extra-chromosomal fragment migrating with an apparent size of 815 kbp. This is the first time that the genome size of C. ruminantium has been determined and the first demonstration of an extrachromosomal element.

journal_name

Ann N Y Acad Sci

authors

de Villiers EP,Brayton KA,Zweygarth E,Allsopp BA

doi

10.1111/j.1749-6632.1998.tb11064.x

subject

Has Abstract

pub_date

1998-06-29 00:00:00

pages

313-20

eissn

0077-8923

issn

1749-6632

journal_volume

849

pub_type

杂志文章
  • Transition to turbulence under low-pressure turbine conditions.

    abstract::In this paper, the topic of laminar to turbulent flow transition, as applied to the design of gas turbines, is discussed. Transition comes about when a flow becomes sufficiently unstable that the orderly vorticity structure of the laminar layer becomes randomly oriented. Vorticity with a streamwise component leads to ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2001.tb05841.x

    authors: Simon TW,Kaszeta RW

    更新日期:2001-05-01 00:00:00

  • Potential role of recombinant annexin II in diabetic vascular injury.

    abstract::Hyperinsulinemia and hyperglycemia have been associated with vascular injury such as atherosclerosis in diabetes mellitus. Recently, annexin II, a member of annexin family proteins, has been found to work as co-receptor on endothelial cells for plasminogen and tissue plasminogen activator, facilitating plasmin generat...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2001.tb03953.x

    authors: Ishii H,Yoshida M,Hajjar KA,Tanaka A,Yasukochi Y,Shimokado K,Numano F

    更新日期:2001-12-01 00:00:00

  • The porous boundaries between explicit and implicit memory: behavioral and neural evidence.

    abstract::Explicit memory refers to the conscious retrieval of past information or experiences, whereas implicit memory refers to an unintentional or nonconscious form of retrieval. Much of the literature in cognitive psychology and cognitive neuroscience has focused on differences between explicit and implicit memory, and the ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2010.05946.x

    authors: Dew ITZ,Cabeza R

    更新日期:2011-04-01 00:00:00

  • ER stress induces the expression of Jpk, which inhibits cell cycle progression in F9 teratocarcinoma cell.

    abstract::Jopock (Jpk), a transacting factor associated with the position-specific regulatory element of murine Hoxa-7, has shown to induce cell death in both prokaryotic and eukaryotic cells when introduced and overexpressed. Since Jpk protein harbors a transmembrane domain (TM) and a putative endoplasmic reticulum (ER) -reten...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1397.011

    authors: Kim HS,Kong KA,Chung H,Park S,Kim MH

    更新日期:2007-01-01 00:00:00

  • Survival and complications in thalassemia.

    abstract::The life expectancy of patients with thalassemia major has significantly increased in recent years, as reported by several groups in different countries. However, complications are still frequent and affect the patients' quality of life. In a recent study from the United Kingdom, it was found that 50% of the patients ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1345.006

    authors: Borgna-Pignatti C,Cappellini MD,De Stefano P,Del Vecchio GC,Forni GL,Gamberini MR,Ghilardi R,Origa R,Piga A,Romeo MA,Zhao H,Cnaan A

    更新日期:2005-01-01 00:00:00

  • Genetic models to study quiescent stem cells and their niches.

    abstract::Hematopoietic stem cells (HSC) have been defined by their ability to establish long-term hematopoiesis in myelo-ablated hosts. Prospective isolation using combinations of cell-surface markers and/or dye exclusion can yield highly purified and nearly homogeneous phenotypically defined cells that repopulate irradiated h...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.2009.04608.x

    authors: Schaniel C,Moore KA

    更新日期:2009-09-01 00:00:00

  • Giant viruses: spore-like missing links between Rickettsia and mitochondria?

    abstract::Phyloproteomics indicate common viral origin from ancient cells before archaea, bacteria, and eukaryota split and subsequent size and complexity reductions occurred. Further independent evidence for the cellular origin of viruses is reviewed for the virus order Megavirales, focusing on the family Poxviridae. Megaviral...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.14022

    authors: Seligmann H

    更新日期:2019-07-01 00:00:00

  • Ambulatory blood pressure monitoring. Application to clinical medicine and antihypertension medication trials.

    abstract::The wide use of ABPM has resulted in greater appreciation of the circadian time structure of BP variability and its clinical relevance. It is now recognized that the day-night change in BP results from an interplay of circadian rhythms in neurohumoral mechanisms coupled with temporal patterns in physical activity and ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1996.tb26724.x

    authors: Smolensky MH,Portaluppi F

    更新日期:1996-08-15 00:00:00

  • Song-induced gene expression: a window on song auditory processing and perception.

    abstract::We review here evidence that a large portion of the caudomedial telencephalon of songbirds, distinct from the song control circuit, is involved in the perceptual processing of birdsong. When songbirds hear song, a number of caudomedial pallial areas are activated, as revealed by expression of the activity-dependent ge...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1298.021

    authors: Mello CV,Velho TA,Pinaud R

    更新日期:2004-06-01 00:00:00

  • Stem cell plasticity in muscle and bone marrow.

    abstract::Recent discoveries have demonstrated the extraordinary plasticity of tissue-derived stem cells, raising fundamental questions about cell lineage relationships and suggesting the potential for novel cell-based therapies. We have examined this phenomenon in a potential reciprocal relationship between stem cells derived ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2001.tb03591.x

    authors: Goodell MA,Jackson KA,Majka SM,Mi T,Wang H,Pocius J,Hartley CJ,Majesky MW,Entman ML,Michael LH,Hirschi KK

    更新日期:2001-06-01 00:00:00

  • Experimental and clinical results in shock lung treatment with vitamin E.

    abstract::The adult respiratory distress syndrome (ARDS) is not confined only to late shock states but is a typical finding in intoxications with such oxidizing agents as O3, NO2, chlorate, or paraquat. Under experimental conditions, alpha-tocopherol in a high enteric dosage (2 x 50 mg/kg) was able to prevent alterations of pul...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 临床试验,杂志文章

    doi:10.1111/j.1749-6632.1982.tb31278.x

    authors: Wolf HR,Seeger HW

    更新日期:1982-01-01 00:00:00

  • Methamphetamine dependence: medication development efforts based on the dual deficit model of stimulant addiction.

    abstract::Converging lines of evidence indicate that withdrawal from prolonged exposure to stimulants and alcohol results in synaptic deficits of both dopamine (DA) and serotonin (5-HT). According to the dual deficit model proposed by the authors, DA dysfunction during cocaine or alcohol withdrawal underlies anhedonia and psych...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2000.tb05185.x

    authors: Rothman RB,Partilla JS,Dersch CM,Carroll FI,Rice KC,Baumann MH

    更新日期:2000-09-01 00:00:00

  • Endotoxin-induced neutrophil adherence to endothelium: relationship to CD11b/CD18 and L-selectin expression and matrix disruption.

    abstract::The injury to vascular endothelium seen in severe bacterial infection may be mediated by neutrophil-derived enzymes. Neutrophil adhesion to endothelium, a prerequisite for this process, is mediated sequentially by the leukocyte adhesion molecules L-selectin and the beta 2 integrins, including CD11b/CD18. We have explo...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1994.tb39799.x

    authors: Finn A,Strobel S,Levin M,Klein N

    更新日期:1994-05-28 00:00:00

  • Differential thermosensor control of thermoregulatory grooming, locomotion, and relaxed postural extension.

    abstract::The rat displays three behaviors when surface and/or deep body temperatures rise above their normal levels: grooming, which spreads saliva over the fur to cool by evaporation; locomotion, which results in escape to a cooler environment; and relaxed postural extension, which reduces heat production by muscle activity a...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1988.tb38620.x

    authors: Roberts WW

    更新日期:1988-01-01 00:00:00

  • Dehydroepiandrosterone enhances influenza immunization in aged mice.

    abstract::The effect of DHEA administration on the age-associated decline in immunity against influenza vaccine was studied. Increased humoral response was observed in 16- and 24-month-old mice immunized by live A/PR/8/34 (H1N1) influenza virus following DHEA treatment (a single injection adjacent to immunization). Furthermore,...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1995.tb17391.x-i1

    authors: Danenberg HD,Ben-Yehuda A,Zakay-Rones Z,Friedman G

    更新日期:1995-12-29 00:00:00

  • Acute phase glycoproteins: bystanders or participants in carcinogenesis?

    abstract::Acute phase proteins (APPs) are a group of serum proteins that undergo dramatic changes in concentration during times of inflammation. Many APPs are heavily glycosylated, and their sugar content and complexity change in the presence of cancer-induced chronic inflammation. These changes in glycosylation are currently b...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2011.06420.x

    authors: Dempsey E,Rudd PM

    更新日期:2012-04-01 00:00:00

  • A dual spinal cord lesion paradigm to study spinal locomotor plasticity in the cat.

    abstract::After a complete spinal cord injury (SCI) at the lowest thoracic level (T13), adult cats trained to walk on a treadmill can recover hindlimb locomotion within 2-3 weeks, resulting from the activity of a spinal circuitry termed the central pattern generator (CPG). The role of this spinal circuitry in the recovery of lo...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2012.06823.x

    authors: Martinez M,Rossignol S

    更新日期:2013-03-01 00:00:00

  • Genetic and phenotypic heterogeneity in ovarian failure: overview of selected candidate genes.

    abstract::Ovarian failure is heterogeneous, both in cause and in phenotype. Women whose menses cease prior to age 40 years and have menopausal FSH levels are traditionally considered by clinicians to have the diagnosis of "premature ovarian failure" (POF). In fact, however, the ovarian function in this disorder is in reality a ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1196/annals.1429.019

    authors: Simpson JL

    更新日期:2008-01-01 00:00:00

  • The esophageal mucosa and submucosa: immunohistology in GERD and Barrett's esophagus.

    abstract::This paper presents commentaries on the microscopic morphology of esophageal squamous epithelium; the frequency of duplication of the muscularis mucosae (MM) in Barrett's esophagus (BE); the significance of multilayered epithelium; whether cells in the lamina propria reflect those in the epithelium; how stem cells are...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.12241

    authors: Appelman HD,Streutker C,Vieth M,Neumann H,Neurath MF,Upton MP,Sagaert X,Wang HH,El-Zimaity H,Abraham SC,Bellizzi AM

    更新日期:2013-10-01 00:00:00

  • Arousal regulation, emotional flexibility, medial amygdala function, and the impact of early experience: comments on the paper of Lewis et al.

    abstract::The balance between optimal levels of emotional arousal and cognitive performance reflects the integration of several dopaminergically and adrenergically regulated neural systems. The amygdalar system is a key region for gating stimulation to cortical regions and the medial amygdala appears to play an especially key r...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 评论,杂志文章,评审

    doi:10.1196/annals.1376.018

    authors: Mayes LC

    更新日期:2006-12-01 00:00:00

  • Investigating metabolic regulation using targeted neuromodulation.

    abstract::The central nervous system (CNS) plays a vital role in regulating energy balance and metabolism. Over the last 50 years, studies in animal models have allowed us to identify critical CNS regions involved in these processes and even crucial cell populations. Now, techniques for genetically and anatomically targeted man...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.13468

    authors: Devarakonda K,Stanley S

    更新日期:2018-01-01 00:00:00

  • IGF-II binding to insulin receptor isoform A induces a partially different gene expression profile from insulin binding.

    abstract::Insulin receptor isoform A (IR-A) is a fetal insulin receptor isoform that is overexpressed in cancer. We investigated whether insulin-like growth factor (IGF)-II may elicit a different gene expression response from insulin in cells expressing only IR-A and lacking IGF-I receptor (R-/IR-A cells). Cells were stimulated...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1322.053

    authors: Pandini G,Conte E,Medico E,Sciacca L,Vigneri R,Belfiore A

    更新日期:2004-12-01 00:00:00

  • The induction of tolerance to allergenic chemicals.

    abstract::The first recognition of tolerance and partial tolerance to attempted sensitization with simple allergenic chemicals is described. A proper designation would be the Frei-Sulzbeger-Chase phenomenon. Coupling with self occurs in these experiments; there is not only resistance to developing contactant-type sensitivity bu...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1982.tb36110.x

    authors: Chase MW

    更新日期:1982-01-01 00:00:00

  • Modulation of noradrenaline release by activation of presynaptic beta-adrenoceptors in the cardiovascular system.

    abstract::Peripheral sympathetic nerve terminals in many tissues, but not all, are endowed with beta-adrenoceptors. Activation of these result in an enhancement of noradrenaline release evoked by electrical nerve stimulation. These so-called presynaptic beta-adrenoceptors are possibly located on the outer surface of the varicos...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1990.tb32018.x

    authors: Nedergaard OA,Abrahamsen J

    更新日期:1990-01-01 00:00:00

  • The anatomy and cell biology of peripheral myelin protein-22.

    abstract::The gain of function phenotypes exhibited by the heterozygous Tr, Tr-J, and CMT1A mutations indicate that these mutations interfere with more than the function of a single PMP22 allele. The identification of proteins that interact with PMP22 and that are sensitive both to stoichiometry and the effects of the mutations...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:

    authors: Snipes GJ,Orfali W,Fraser A,Dickson K,Colby J

    更新日期:1999-09-14 00:00:00

  • The design of orally active iron chelators.

    abstract::It is now generally accepted that it is not possible to design iron(III)-selective hexadentate chelators with high oral bioavailability. In order to achieve suitable levels of oral activity for the treatment of systemic iron overload either tridentate or bidentate molecules need to be investigated. There are a number ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1196/annals.1345.017

    authors: Hider RC,Zhou T

    更新日期:2005-01-01 00:00:00

  • The neuroethology of friendship.

    abstract::Friendship pervades the human social landscape. These bonds are so important that disrupting them leads to health problems, and difficulties forming or maintaining friendships attend neuropsychiatric disorders like autism and depression. Other animals also have friends, suggesting that friendship is not solely a human...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/nyas.12315

    authors: Brent LJ,Chang SW,Gariépy JF,Platt ML

    更新日期:2014-05-01 00:00:00

  • The substance P and somatostatin interferon-gamma immunoregulatory circuit.

    abstract::Murine schistosomiasis mansoni is a parasitic disease in which flukes living in the portal vein of the host produce ova that deposit in the liver and intestines. In these organs, ova release antigens that induce chronic, focal granulomatous inflammation. IFN-gamma is an inflammatory cytokine important in macrophage ac...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.1998.tb09592.x

    authors: Weinstock JV,Elliott D

    更新日期:1998-05-01 00:00:00

  • Pathological findings of HIV-associated cardiovascular disease.

    abstract::More effective therapies have improved survival times of HIV+ patients, resulting in a higher prevalence of long-term complications of the disease. This review focuses on HIV-associated cardiovascular pathology, correlating the morphologic findings to clinical syndromes of HIV disease/AIDS. ...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章,评审

    doi:10.1111/j.1749-6632.2001.tb03901.x

    authors: d'Amati G,di Gioia CR,Gallo P

    更新日期:2001-11-01 00:00:00

  • Visual versus auditory memory stimulation in patients with probable Alzheimer's disease. A PET study with 18 FDG.

    abstract::Patients with probable Alzheimer's disease were either stimulated with a continuous visual recognition task (n = 18) or with a continuous auditory recognition task during PET measurement with 18 FDG. The PET scanner was a Siemens CTI, ECAT EXACT with 5 mm transaxial and 6 mm axial resolution. The global stimulation ef...

    journal_title:Annals of the New York Academy of Sciences

    pub_type: 杂志文章

    doi:10.1111/j.1749-6632.1996.tb34424.x

    authors: Kessler J,Ghaemi M,Mielke R,Herholz K,Heiss WD

    更新日期:1996-01-17 00:00:00