Plasma cysteine/cystine reduction potential correlates with plasma creatinine levels in chronic kidney disease.

Abstract:

BACKGROUND/AIMS:Oxidative stress has been considered a nontraditional risk factor for cardiovascular disease in the chronic kidney disease (CKD) population, possibly triggered by uremic toxicity. METHODS:A chromatographic method with coulometric detection was adapted to directly and simultaneously determine cysteine (Cys) and cystine (Cyss) in plasma samples. Healthy subjects and CKD subjects in different stages were analyzed. The free Cys and free Cyss levels in their plasma were determined, and the reduction potential [Eh(Cyss/2Cys)] was calculated with the Nernst equation. RESULTS:Healthy plasma presented Eh(Cyss/2Cys) of -123 ± 7 mV. Plasma Eh(Cyss/2Cys) correlated significantly with creatinine levels (p < 0.0001, r = 0.62). CONCLUSION:Plasma Eh(Cyss/2Cys) correlated with increased levels of plasma creatinine, supporting the view that uremia triggers oxidative stress. In addition, it may be used as a quantitative oxidative stress biomarker in uremic conditions.

journal_name

Blood Purif

journal_title

Blood purification

authors

Rodrigues SD,Batista GB,Ingberman M,Pecoits-Filho R,Nakao LS

doi

10.1159/000342627

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

231-7

issue

3-4

eissn

0253-5068

issn

1421-9735

pii

000342627

journal_volume

34

pub_type

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