Anticoagulation of diethyl citrate and its comparison with sodium citrate in an animal model.

Abstract:

AIMS:To improve the side effects caused by sodium citrate (Na(3)Cit), the anticoagulant effects of diethyl citrate (Et(2)Cit) were investigated. METHODS:The in vitro anticoagulant effects and dissociation capacity of the chelate of Et(2)Cit with calcium ions were compared with those of Na(3)Cit in rabbits. RESULTS:The activated coagulation time test showed that blood clotting time exceeded 1,200 s when the concentrations of Et(2)Cit and Na(3)Cit were greater than 87.2 and 8.72 mmol/l, respectively. The concentrations of free calcium ions in blood c(Ca(2+)) were reduced when Et(2)Cit was injected into the rabbits. CONCLUSIONS:Et(2)Cit reduces the concentration of ionized Ca(2+) in blood and has anticoagulant effects. The dissociation of the chelate of Et(2)Cit with Ca(2+) was faster than that of Na(3)Cit with Ca(2+) within 10 min after injection. The recovery speed of blood calcium concentration with Et(2)Cit was more rapid than that with Na(3)Cit. The findings show that Et(2)Cit prevents hypocalcemia.

journal_name

Blood Purif

journal_title

Blood purification

authors

Chen Z,Chen B,Yao XQ,Gui BS,Ou Y,Ouyang JM

doi

10.1159/000330891

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

30-6

issue

1-3

eissn

0253-5068

issn

1421-9735

pii

000330891

journal_volume

33

pub_type

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