Bioartificial kidney alters cytokine response and hemodynamics in endotoxin-challenged uremic animals.


:The mortality from sepsis complicated by renal failure remains extremely high despite the application of modern renal replacement therapy. This study investigated whether treatment with a bioartificial kidney consisting of a hemofilter in a continuous venovenous hemofiltration circuit (CVVH) with a cartridge containing renal proximal tubule cells, also called the Renal Tubule Assist Device (RAD), would alter the course of sepsis in an animal model. The RAD has been previously characterized in vitro and ex vivo and provides transport, metabolic and endocrine activity. Mongrel dogs (n = 10) underwent surgical nephrectomy and 48 h later were treated with CVVH and either a RAD containing cells (n = 5) or an identically prepared sham cartridge (n = 5). After 4 h of therapy, intravenous endotoxin 2 mg/kg was infused over 1 h to simulate gram-negative septic shock. Data on blood pressure, cardiac output and systemic markers of inflammation were collected. Mean peak levels of an anti- inflammatory cytokine, IL-10, were significantly higher in cell-treated animals (15.25 vs. 6.29 ng/ml; p = 0.037), and mean arterial pressures were higher in cell-treated versus sham-treated animals (p < 0.04). We have demonstrated that treatment of an animal model of endotoxin shock and renal failure with a bioartificial kidney has measurable effects on circulating mediators of inflammation and on hemodynamic stability of the challenged animal.


Blood Purif


Blood purification


Fissell WH,Dyke DB,Weitzel WF,Buffington DA,Westover AJ,MacKay SM,Gutierrez JM,Humes HD




Has Abstract


2002-01-01 00:00:00














  • Initiation Condition of Hemodialysis Is Independently Associated with All-Cause Mortality in Maintenance Hemodialysis Patients: A Retrospective Study.

    abstract:BACKGROUND:Despite the progression of dialysis techniques,  the mortality of hemodialysis (HD) patients is still high in China. Here, a retrospective study was performed to investigate the neglected risk factors of all-cause mortality during maintenance HD (MHD). METHODS:We investigated 117 MHD patients who died betwe...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Wang C,Yang Y,Yuan F,Li HQ,He LY,Liu D,Liu YX,Zhou A,Chen X,Liu FY,Peng YM,Liu H

    更新日期:2019-01-01 00:00:00

  • Effect of Furosemide on Urinary Oxygenation in Patients with Septic Shock.

    abstract:BACKGROUND:Renal medullary hypoxia precedes the development of acute kidney injury in experimental sepsis and can now be assessed by continuous measurement of urinary oxygen tension (PuO2). OBJECTIVES:We aimed to test if PuO2 measurements in patients with septic shock would be similar to those shown in experimental se...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Osawa EA,Cutuli SL,Bitker L,Canet E,Cioccari L,Iguchi N,Lankadeva YR,Eastwood GM,Evans RG,May CN,Bellomo R

    更新日期:2019-01-01 00:00:00

  • Prevention of Intradialytic Hypotension with Intermittent Back-Filtrate Infusion Haemodiafiltration: Insights into the Underlying Mechanism.

    abstract:BACKGROUND:Intradialytic hypotension (IDH) is a major challenge to safely performing haemodialysis. Blood volume depletion due to fluid removal is a major cause of hypotension, so more emphasis should be placed on finding alternative modalities to traditional constant rate ultrafiltration. SUMMARY:Intermittent back-fi...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Koda Y,Aoike I

    更新日期:2019-01-01 00:00:00

  • Haemolipodialysis.

    abstract::Haemodialysis is associated with increased oxidant stress. This appears to be due to (1) an increased production of free radicals during haemodialysis, (2) a net reduction of many antioxidants and (3) factors intrinsic to the uremic state. These alterations can lead to cardiovascular disease and many of the pathologie...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Wratten ML,Navino C,Tetta C,Verzetti G

    更新日期:1999-01-01 00:00:00

  • Antibodies to periodontal organisms are associated with decreased kidney function. The Dental Atherosclerosis Risk In Communities study.

    abstract:BACKGROUND/AIMS:Increasing evidence suggests that clinical signs of periodontal disease are independently associated with renal impairment. However, no studies have examined the possible linkage of kidney disease with serum antibody to oral pathogens. METHODS:The periodontal disease status was assessed in an older com...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Kshirsagar AV,Offenbacher S,Moss KL,Barros SP,Beck JD

    更新日期:2007-01-01 00:00:00

  • Plasmapheresis for the Treatment of Acute Pancreatitis due to Severe Hypertriglyceridemia.

    abstract::Severe hypertriglyceridemia (HTG) is associated with acute pancreatitis (AP). Treatment options include total plasma exchange (TPE). We report a case of AP due to severe HTG treated with TPE. ...

    journal_title:Blood purification



    authors: Ruiz-Rodríguez JC,Chiscano-Camón LS,Palmada C,Pons V,Ferrer R

    更新日期:2020-11-24 00:00:00

  • Red blood cell density distribution in uremic patients on acetate and bicarbonate hemodialysis.

    abstract::Renal anemia is the result of reduced erythropoietin (EPO) biosynthesis in the diseased kidney and also in part the result of a reduced life span of red blood cells (RBCs). An increase in density and a decrease in enzyme equipment (aspartate aminotransferase; GOT) of RBCs reflect cell age. In the following study, the ...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Hampl H,Riedel E,Wendel G,Scigalla P

    更新日期:1990-01-01 00:00:00

  • Regulation of the response to bacterial lipopolysaccharide by endogenous and exogenous lipopolysaccharide binding proteins.

    abstract::Bactericidal/permeability-increasing protein (BPI) is a natural constituent of human neutrophils. Recombinant BPI has been shown to bind to bacterial lipopolysaccharide (LPS), and to neutralize the ability of LPS to stimulate inflammatory cells in vitro and in vivo. BPI shares sequence homology and immunocrossreactivi...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Marra MN,Thornton MB,Snable JL,Leong S,Lane J,Wilde CG,Scott RW

    更新日期:1993-01-01 00:00:00

  • A target-orientated algorithm for regional citrate-calcium anticoagulation in extracorporeal therapies.

    abstract:BACKGROUND:Citrate anticoagulation offers several advantages in comparison to conventional anticoagulation. Most algorithms for regional citrate-calcium anticoagulation are based on citrate and calcium chloride infusion coupled in a fixed proportion to the blood flow without considering the hematocrit (Hct)/plasma flow...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Brandl M,Strobl K,Hartmann J,Kellner K,Posnicek T,Falkenhagen D

    更新日期:2012-01-01 00:00:00

  • Long-term on-line hemodiafiltration reduces predialysis beta-2-microglobulin levels in chronic hemodialysis patients.

    abstract:BACKGROUND:Hemodiafiltration (HDF) is effective in delaying the surgical need for carpal tunnel syndrome in chronic hemodialysis patients, however, predialysis beta(2)-microglobulin levels were not reduced in most short-term studies. The aim of this study was to assess the effect of long-term and differing frequencies ...

    journal_title:Blood purification

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Lin CL,Yang CW,Chiang CC,Chang CT,Huang CC

    更新日期:2001-01-01 00:00:00

  • Anorexia and malnutrition in hemodialysis patients.

    abstract::Malnutrition, which is common in maintenance hemodialysis (HD) patients, is strongly associated with increased morbidity and mortality. Protein requirements in HD patients are increased twice or more compared to those of healthy subjects. Underdialysis leads to anorexia with low protein and energy intake in relation t...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Bergström J

    更新日期:1992-01-01 00:00:00

  • Effects of calcitriol on type 5b tartrate-resistant acid phosphatase and interleukin-6 in secondary hyperparathyroidism.

    abstract:BACKGROUND/AIMS:Secondary hyperparathyroidism (SHP) is characterized by high bone turnover and elevated serum bone remodeling markers. Elevation of serum interleukin-6 (IL-6) levels is also characteristic of end-stage renal disease. This study investigates the effects of intravenous calcitriol on serum bone resorptive ...

    journal_title:Blood purification

    pub_type: 临床试验,杂志文章,多中心研究


    authors: Lu KC,Tseng CF,Wu CC,Yeung LK,Chen JS,Chao TY,Janckila AJ,Yam LT,Chu P

    更新日期:2006-01-01 00:00:00

  • Anticoagulation for continuous arteriovenous hemofiltration in children.

    abstract::Continuous arteriovenous hemofiltration requires continuous anticoagulation to prevent early hemofilter clotting. We used heparin given continuously in the arterial line of the extracorporeal circuit as anticoagulant in children with initially normal coagulation status, and heparin and/or prostacyclin in high-risk ble...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Zobel G,Trop M,Muntean W,Ring E,Gleispach H

    更新日期:1988-01-01 00:00:00

  • Current Focuses in Serum Lipid Abnormalities in Dialysis Patients.

    abstract:BACKGROUND:Dyslipidemia is a common metabolic complication in patients with chronic kidney disease and is detected as changes in lipoprotein concentrations in serum. SUMMARY:Recently, other features of lipid abnormalities have been described, such as alterations in serum n-3 polyunsaturated fatty acids, cholesterol me...

    journal_title:Blood purification

    pub_type: 评论,杂志文章


    authors: Shoji T

    更新日期:2015-01-01 00:00:00

  • Hemodialysis with a dialyzer loaded with argatroban may be performed in vivo without a systemic anticoagulant.

    abstract:BACKGROUND/AIMS:Anticoagulation in hemodialysis (HD) patients at high risk of bleeding remains an intractable problem. Simulating endothelial cells by releasing anticoagulant on the membrane may be a promising alternative. METHODS:We modified a polyacrylonitrile (PAN) dialyzer by loading argatroban into its membrane a...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Zhang Y,Ning J,Veeraragoo P,Li Y,Dai S

    更新日期:2012-01-01 00:00:00

  • Plasma C-reactive protein in hemodialysis patients: a cross-sectional, longitudinal clinical survey.

    abstract::In hemodialysis patients, C-reactive protein (CRP), an acute-phase reactant, is a sensitive and independent marker of malnutrition, anemia, and amyloidosis. The aim of the present studies was to evaluate CRP and interleukin 6 levels in plasma samples from long-term hemodialysis patients on different extracorporeal mod...

    journal_title:Blood purification

    pub_type: 杂志文章,多中心研究


    authors: Panichi V,Migliori M,De Pietro S,Metelli MR,Taccola D,Perez R,Palla R,Rindi P,Cristofani R,Tetta C

    更新日期:2000-01-01 00:00:00

  • Adequacy of peritoneal dialysis--where are we going?

    abstract::The present working hypothesis regarding adequacy issues in peritoneal dialysis is that dialysis is a major determinant of protein intake and therefore nutritional status in these patients. Present data however is controversial. This is in part due to differences in methodology and the calculations used for the data p...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Burkart JM

    更新日期:1995-01-01 00:00:00

  • Prevention and management of dialysis catheter complications in the intensive care unit.

    abstract::Dialysis dependence at hospital discharge after acute kidney injury (AKI) requiring renal replacement therapy (RRT) in the intensive care unit (ICU) is found in 10-15% of survivors. In case of severe AKI in the ICU, it is necessary to reconcile two objectives: the creation of an adequate temporary angioaccess for RRT ...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Wilson P,Lertdumrongluk P,Leray-Moragués H,Chenine-Koualef L,Patrier L,Canaud B

    更新日期:2012-01-01 00:00:00

  • Peritoneal membrane failure in peritoneal dialysis patients.

    abstract::A review is given of the conditions associated with peritoneal membrane failure, and the possible causes. Ultrafiltration failure is the most important manifestation. It is mostly associated with high transport rates of low molecular weight solutes suggesting the presence of a large vascular surface area. Enlargement ...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Krediet RT,Van Esch S,Smit W,Michels WM,Zweers MM,Ho-Dac-Pannekeet MM,Struijk DG

    更新日期:2002-01-01 00:00:00

  • The Rise of Expanded Hemodialysis.

    abstract::The low water permeability feature of original cellulosic membranes was considered an advantage in the absence of dialysis equipment that are capable of controlling water removal. The advent of ultrafiltration control systems led to the development and use of high-flux (HF) membranes that allowed improved middle molec...

    journal_title:Blood purification

    pub_type: 社论


    authors: Ronco C

    更新日期:2017-01-01 00:00:00

  • Calculating Standard Kt/V during Hemodialysis Based on Urea Mass Removed.

    abstract:BACKGROUND/AIMS:We derived a novel equation for calculating weekly urea standard Kt/V (stdKt/V) during hemodialysis (HD) based on urea mass removed, comparable to the approach during peritoneal dialysis. METHODS:Theoretical consideration of urea mass balance during HD led to the following equation for stdKt/V, namely,...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Leypoldt JK,Vonesh EF

    更新日期:2019-01-01 00:00:00

  • Renal replacement therapy in geriatric end-stage renal disease patients: a clinical approach.

    abstract::The number of geriatric patients on dialysis is increasing. This is due to demographic factors, a wider acceptance of elderly patients on dialysis, and an earlier start of dialysis in this patient group. Recent studies have questioned the effect of dialysis on quality of life in elderly patients with severe comorbidit...

    journal_title:Blood purification

    pub_type: 杂志文章,评审


    authors: Kooman JP,Cornelis T,van der Sande FM,Leunissen KM

    更新日期:2012-01-01 00:00:00

  • A zebrafish model for uremic toxicity: role of the complement pathway.

    abstract::Many organic solutes accumulate in end-stage renal disease (ESRD) and some are poorly removed with urea-based prescriptions for hemodialysis. However, their toxicities have been difficult to assess. We have employed an animal model, the zebrafish embryo, to test the toxicity of uremic serum compared to control. Serum ...

    journal_title:Blood purification

    pub_type: 临床试验,杂志文章


    authors: Berman N,Lectura M,Thurman J,Reinecke J,Raff AC,Melamed ML,Reinecke J,Quan Z,Evans T,Meyer TW,Hostetter TH

    更新日期:2013-01-01 00:00:00

  • Are both psychological and physical dimensions in health-related quality of life associated with mortality in hemodialysis patients: a 7-year Taiwan cohort study.

    abstract:BACKGROUND:Psychological depression and physical disability are closely correlated in hemodialysis patients. A retrospective cohort study was conducted to examine the independent association of physical and psychological functioning with mortality in a hemodialysis cohort in Taiwan. METHODS:A total of 888 stable hemod...

    journal_title:Blood purification

    pub_type: 杂志文章,多中心研究


    authors: Peng YS,Chiang CK,Hung KY,Chang CH,Lin CY,Yang CS,Chen TW,Hsia CC,Chen DL,Hsu WD,Chang CF,Wu KD,Lin RP,Tsai TJ,Chen WY

    更新日期:2010-01-01 00:00:00

  • Health-Related Quality of Life and Its Factors of Hemodialysis Patients in Suzhou, China.

    abstract:BACKGROUND:To identify factors of health-related quality of life (HRQOL) of Chinese end-stage renal disease patients undergoing hemodialysis (HD). METHODS:In all, 315 HD patients were recruited from a tertiary hospital in Suzhou, -China. Each patient completed the Kidney Disease Quality of Life Short Form. It generate...

    journal_title:Blood purification

    pub_type: 临床试验,杂志文章


    authors: Pan CW,Wu Y,Zhou HJ,Xu BX,Wang P

    更新日期:2018-01-01 00:00:00

  • Benefit of glucose-free dialysis solutions on glucose and lipid metabolism in peritoneal dialysis patients.

    abstract:BACKGROUND:Glucose absorbed from conventional peritoneal dialysis (PD) solutions contributes to unfavorable metabolic effects. Its replacement with a glucose-free osmotic agent such as icodextrin (ID) or amino acids (AA) may have some benefit on glucose and lipid metabolism. METHODS:Serum lipids, insulin sensitivity a...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Martikainen T,Teppo AM,Gronhagen-Riska C,Ekstrand A

    更新日期:2005-01-01 00:00:00

  • Kidney transplantation improves sleep-related breathing in hemodialysis patients.

    abstract:AIMS:To assess whether kidney transplantation improves sleep-related breathing disorders associated with hemodialysis. METHODS:A prospective study was carried out using full-night polysomnography. Nine hemodialysis patients (7 men and 2 women, aged 42 +/- 16.2 years, BMI 26 +/- 3.7) who later received a successful kid...

    journal_title:Blood purification

    pub_type: 临床试验,杂志文章


    authors: Jurado-Gámez B,Martin-Malo A,Rodriguez-Benot A,Muñoz-Cabrera L,Cosano Povedano A,Aljama P

    更新日期:2008-01-01 00:00:00

  • Psychological aspects of low protein diet therapy.

    abstract::One of the main problems in low protein diet is patient's noncompliance and the risk of both depression and reduced quality of life. A pilot study investigated the life-style and the state of mood before and during low protein diet. The results show a deterioration in the life-style and the state of mood. Conclusions ...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Haeberle KN

    更新日期:1989-01-01 00:00:00

  • Methylation status of immune response genes promoters in cell-free DNA differs in hemodialyzed patients with diabetic nephropathy according to the intensity of anemia therapy.

    abstract:BACKGROUND:Anemia is a major complication of end-stage renal disease. Hemodialysis itself is regarded as a stimulus activating inflammation. Pro-inflammatory cytokines are able to suppress erythropoiesis. In this pilot study, we analyzed the changes in methylation status of promoters of immune response genes in cell-fr...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Korabecna M,Pazourkova E,Horinek A,Mokrejsova M,Tesar V

    更新日期:2013-01-01 00:00:00

  • Why assistive technology is needed for probing of dry weight.

    abstract::Despite advancements in dialysis therapy, the subjective clinical examination still remains the standard of care in managing volume removal in chronic dialysis. While there is no definitive trial establishing that dry weight management guided by an assistive technology is superior to the clinical method, there is ampl...

    journal_title:Blood purification

    pub_type: 杂志文章


    authors: Sinha AD

    更新日期:2011-01-01 00:00:00