Abstract:
:Many organic solutes accumulate in end-stage renal disease (ESRD) and some are poorly removed with urea-based prescriptions for hemodialysis. However, their toxicities have been difficult to assess. We have employed an animal model, the zebrafish embryo, to test the toxicity of uremic serum compared to control. Serum was obtained from stable ESRD patients predialysis or from normal subjects. Zebrafish embryos 24 h postfertilization were exposed to experimental media at a water:human serum ratio of 3:1. Those exposed to serum from uremic subjects had significantly reduced survival at 8 h (19 ± 18 vs. 94 ± 6%, p < 0.05, uremic serum vs. control, respectively). Embryos exposed to serum from ESRD subjects fractionated at 50 kDa showed significantly greater toxicity with the larger molecular weight fraction (83 ± 11 vs. 7 ± 17% survival, p < 0.05, <50 vs. >50 kDa, respectively). Heating serum abrogated its toxicity. EDTA, a potent inhibitor of complement by virtue of calcium chelation, reduced the toxicity of uremic serum compared to untreated uremic serum (96 ± 5 vs. 28 ± 20% survival, p < 0.016, chelated vs. nonchelated serum, respectively). Anti-factor B, a specific inhibitor of the alternative complement pathway, reduced the toxicity of uremic serum, compared to untreated uremic serum (98 ± 6 vs. 3 ± 9% survival, p < 0.016, anti-factor B treated vs. nontreated, respectively). Uremic serum is thus more toxic to zebrafish embryos than normal serum. Furthermore, this toxicity is associated with a fraction of large size, is inactivated by heat, and is reduced by both specific and nonspecific inhibitors of complement activation. Together these data lend support to the hypothesis that at least some uremic toxicities may be mediated by complement.
journal_name
Blood Purifjournal_title
Blood purificationauthors
Berman N,Lectura M,Thurman J,Reinecke J,Raff AC,Melamed ML,Reinecke J,Quan Z,Evans T,Meyer TW,Hostetter THdoi
10.1159/000348456subject
Has Abstractpub_date
2013-01-01 00:00:00pages
265-269issue
4eissn
0253-5068issn
1421-9735journal_volume
35pub_type
临床试验,杂志文章abstract:INTRODUCTION:Glycated hemoglobin is used to assess diabetic control although its accuracy in dialysis has been questioned. How does it compare to the Continuous Glucose Monitoring System (CGMS) in peritoneal dialysis (PD) patients? METHODS:We conducted a retrospective analysis of 60 insulin-treated diabetic patients o...
journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000439242
更新日期:2016-01-01 00:00:00
abstract::During hemodialysis, amino acids (AA) are lost in the ultrafiltrate with consequent modification of their plasma profile. The aim of this cross-sectional study was to evaluate intradialytic changes of plasma AA levels during a single session of hemodiafiltration with endogenous reinfusion (HFR) versus acetate-free bio...
journal_title:Blood purification
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doi:10.1159/000320133
更新日期:2010-01-01 00:00:00
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doi:10.1159/000066958
更新日期:2002-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000342627
更新日期:2012-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000360267
更新日期:2014-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000496660
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abstract::Secondary hyperparathyroidism (SHPT) occurs in patients with chronic renal failure complicated with renal bone disease and soft tissue/vascular calcification. In dialysis patients with severe SHPT, medical treatment may fail and parathyroidectomy (PTX) is indicated for definitive treatment. Severe hypocalcemia from hu...
journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000479616
更新日期:2017-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000337101
更新日期:2012-01-01 00:00:00
abstract::To evaluate objectively the effects of recombinant human erythropoietin (rHuEPO) administration on nutritional status in stable dialyzed patients, we used urea kinetic modeling (UKM) analysis and dietary protein intake evaluation by dietary assessment. Fifteen patients (9 females, 6 males; mean age 46.9 +/- 15.6 years...
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pub_type: 杂志文章
doi:10.1159/000169982
更新日期:1990-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000338923
更新日期:2012-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000046986
更新日期:2002-01-01 00:00:00
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journal_title:Blood purification
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000046958
更新日期:2001-01-01 00:00:00
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journal_title:Blood purification
pub_type: 临床试验,杂志文章
doi:10.1159/000485962
更新日期:2018-01-01 00:00:00
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journal_title:Blood purification
pub_type: 临床试验,杂志文章
doi:10.1159/000494207
更新日期:2019-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000474931
更新日期:2017-01-01 00:00:00
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journal_title:Blood purification
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1159/000014331
更新日期:1998-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000496641
更新日期:2019-01-01 00:00:00
abstract::Doubt has remained as to whether or not the K/DOQI recommended targets for adequacy of dialysis for peritoneal dialysis patients is appropriate (weekly Kt/V 2 + creatinine clearance 50-60 l/1.73 m(2)). The ADEMEX trial can be interpreted as indicating that lower targets might be acceptable. The HEMO trial, not yet pub...
journal_title:Blood purification
pub_type: 临床试验,杂志文章
doi:10.1159/000067869
更新日期:2003-01-01 00:00:00
abstract::At present the treatment of hepatitis C virus infection (HCV) has improved because of combined treatment with interferon (IFN) and ribavirin. The association of these two drugs has obtained a positive response in 40-50% of naive patients treated. Patients with relapse after monotherapy with IFN also show positive resp...
journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000046996
更新日期:2002-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1159/000170201
更新日期:1995-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000493178
更新日期:2019-01-01 00:00:00
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journal_title:Blood purification
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更新日期:1998-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000218090
更新日期:2009-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000356366
更新日期:2013-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000096400
更新日期:2007-01-01 00:00:00
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journal_title:Blood purification
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1159/000485231
更新日期:2018-01-01 00:00:00
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journal_title:Blood purification
pub_type: 临床试验,杂志文章
doi:10.1159/000076855
更新日期:2004-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章,评审
doi:10.1159/000078699
更新日期:2004-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Blood purification
pub_type: 杂志文章
doi:10.1159/000452755
更新日期:2016-01-01 00:00:00