Abstract:
:Neuropeptide S (NPS) produces several biological actions by activating a formerly orphan GPCR, now named NPS receptor (NPSR). It has been previously demonstrated that NPS stimulates murine leukocyte chemotaxis in vitro. In the present study we investigated the ability of NPS, in comparison with the proinflammatory peptide formyl-Met-Leu-Phe (fMLP), to stimulate human monocyte chemotaxis. At a concentration of 10(-8)M fMLP significantly stimulated chemotaxis. NPS produced a concentration dependent chemotactic action over the concentration range 10(-12) to 10(-5)M. The NPSR antagonists [D-Cys((t)Bu)(5)]NPS, [(t)Bu-D-Gly(5)]NPS and SHA 68 were used to pharmacologically characterize NPS action. Monocyte chemoattractant effect of NPS, but not fMLP, was completely blocked by either peptide antagonists or SHA with the nonpeptide molecule being more potent. None of the NPSR antagonists modified per se random cell migration. Thus, the present study demonstrated that NPS is able to stimulate human monocyte chemotaxis and that this effect is entirely due to selective NPSR activation.
journal_name
Peptidesjournal_title
Peptidesauthors
Filaferro M,Novi C,Ruggieri V,Genedani S,Alboni S,Malagoli D,Caló G,Guerrini R,Vitale Gdoi
10.1016/j.peptides.2012.10.013subject
Has Abstractpub_date
2013-01-01 00:00:00pages
16-20eissn
0196-9781issn
1873-5169pii
S0196-9781(12)00445-7journal_volume
39pub_type
杂志文章相关文献
PEPTIDES文献大全abstract::VIP and related fragments were prepared by the solid-phase method. The peptides were assembled on a benzhydrylamine resin and couplings of the Boc-amino acids were carried out by the symmetrical anhydride method. Cleavage was achieved by treatment with liquid HF and purification was accomplished by successive steps of...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(84)90202-x
更新日期:1984-03-01 00:00:00
abstract::The reperfusion injury salvage kinase (RISK) pathway is a fundamental signal transduction cascade in the cardioprotective mechanism of ischemic postconditioning. In the present study, we examined the cardioprotective role of oxytocin as a postconditioning agent via activation of the RISK pathway (PI3K/Akt and ERK1/2)....
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2016.10.001
更新日期:2016-12-01 00:00:00
abstract::Herein the effect of orexin receptor type-1 antagonist SB-334867 on the development of tolerance to analgesic effects of morphine was studied in rats. To incite tolerance, morphine sulfate was injected intraperitoneally (i.p., 10mg/kg) once a day for 7 days. The tail flick test was used to evaluate antinociceptive eff...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.02.023
更新日期:2012-05-01 00:00:00
abstract::Melanin-concentrating hormone (MCH) is a potent orexigenic and sleep-promoting neuropeptide in mammals produced predominately by hypothalamic neurons which project to a wide variety of brain areas. Several MCH producing neurons contain MCH as the only neuropeptide, while others comprise cocaine- and amphetamine regula...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2017.11.002
更新日期:2018-01-01 00:00:00
abstract::The guinea pig ileum possesses NK-1 and NK-3 tachykinin receptors. As expected, [Pro9]SP and senktide, which are selective agonists of NK-1 and NK-3 receptors, respectively, were found to be highly potent in contracting the guinea pig ileum. Surprisingly, similar observations were made with septide, SP-O-CH3, [Apa9-10...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(92)90125-m
更新日期:1992-03-01 00:00:00
abstract::We performed the first comprehensive peptidomic analysis of neurohormones from hemipteran insects by analyzing the neuropeptides of two major neurohemal organs, namely the corpora cardiaca and abdominal perisympathetic organs. For the experiments we selected four related species of polyphagous stinkbugs (Pentatomidae)...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2007.08.034
更新日期:2008-02-01 00:00:00
abstract::Urotensin II (U-II) is a vasoactive peptide with many potent effects in the cardiorenovascular system. U-II activates a G-protein-coupled receptor termed UT. UT and U-II are highly expressed in the cardiovascular and renal system. Patients with various cardiovascular diseases show high U-II plasma levels. It was demon...
journal_title:Peptides
pub_type: 杂志文章,评审
doi:10.1016/j.peptides.2007.08.029
更新日期:2008-05-01 00:00:00
abstract::A novel 6.6 kDa antifungal peptide (NFAP) from the culture supernatant of the mold, Neosartorya fischeri (anamorf: Aspergillus fischerianus), and its encoding gene were isolated in this study. NFAP is a small, basic and cysteine-rich protein consisting of 57 amino acid residues. It shows 37.9-50% homology to similar p...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2011.06.022
更新日期:2011-08-01 00:00:00
abstract::The melanocortin 4 receptor (MC4R) plays an essential role in weight regulation. Recently we could show that the MC4R is able to form receptor dimers. In the present study we investigated the role of extracellular cysteine residues and the structure of the third extracellular loop for receptor dimerization. None of th...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.02.027
更新日期:2006-02-01 00:00:00
abstract::Interdigestive migrating contractions (IMC) were analyzed for their occurrence interval, duration, migrating velocity, and frequently in long term records obtained by means of chronically implanted force transducers in the main stomach and the extrinsically denervated fundic pouches or in the intact jejunum and the ex...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(81)90035-8
更新日期:1981-01-01 00:00:00
abstract::Bradykinin-potentiating peptides from Bothrops jararaca (Bj) discovered in the early 1960s, were the first natural inhibitors of the angiotensin-converting enzyme (ACE). These peptides belong to a large family of snake venom proline-rich oligopeptides (PROs). One of these peptides, Bj-PRO-9a, was essential for definin...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2013.07.016
更新日期:2013-10-01 00:00:00
abstract::A retrograde facilitation has been demonstrated in the one trial step-down inhibitory avoidance of melanin-concentrating hormone (MCH), when it was infused into rat hippocampal formation. Considering the high density of specific binding sites for the MCH peptide on the hippocampus and the participation of this structu...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00591-5
更新日期:2002-01-01 00:00:00
abstract::Two peptides, with a molecular mass of about 11 kDa and an N-terminal sequence abundant in arginine and glutamine residues, were isolated from wax gourd seeds. The isolation protocol included affinity chromatography on Affi-gel blue gel, ion-exchange chromatography on Mono-S and gel filtration on Superdex 75. The pept...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00271-1
更新日期:2003-01-01 00:00:00
abstract::Immunoreactive alpha-MSH was found in human skin and the skin of numerous other mammals. After hypophysectomy the concentration of alpha-MSH in rat skin showed little change suggesting that the pituitary is not the source of this MSH. In human skin the highest concentration was found in the epidermis and HPLC revealed...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(83)90072-4
更新日期:1983-11-01 00:00:00
abstract::The location of the cells giving rise to the tachykinergic innervation of the rat arcuate nucleus was studied by combining immunohistochemistry and retrograde axonal transport of a protein-gold complex (WGA-ApoHRP-gold). Small volumes (20 nl) of this marker were injected into the arcuate nucleus of the rat. Twenty-fou...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90042-f
更新日期:1993-03-01 00:00:00
abstract::We sought to establish Endothelin (ET-3) role in the central regulation of bile secretion in the rat. The intracerebroventricular (icv) injection of ET-3 evoked a cholestatic or a choleretic effect depending on the administered dose. Lower doses increased bile flow and bicarbonate excretion, whereas higher doses decre...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.02.003
更新日期:2005-07-01 00:00:00
abstract::Which of Y1-Y5 receptors (Rs) mediate NPY's angiogenic activity was studied using Y2R-null mice and R-specific antagonists. In Y2R-null mice, NPY-induced aortic sprouting and in vivo Matrigel capillary formation were decreased by 50%; Y1R-antagonist blocked the remaining response. NPY-induced sprouting was equally inh...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00281-4
更新日期:2003-01-01 00:00:00
abstract::Neuropeptide FF (NPFF) has been shown to act as an endogenous anti-analgesic peptide. In this paper, several peptide analogs of the selective ligand dNP(NMe)AFLFQPQRF-NH(2) modified in the putative address segment, were designed to be selective NPFF(2) receptor probes, synthesized and assayed. One peptide dA(NMe)AAFLF...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2012.07.008
更新日期:2012-09-01 00:00:00
abstract::The effects of arginine vasotocin (AVT), 4-6 ng/kg, on renal function and plasma prolactin (PRL) were determined in conscious American foxhounds undergoing a water diuresis. Intravenous AVT produced an antidiuresis, kaliuresis and inconsistent natriuresis, independently of changes in GFR, blood pressure, heart rate an...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(82)90062-6
更新日期:1982-11-01 00:00:00
abstract::Liver-expressed antimicrobial peptide 2 (LEAP-2) is a 40-residue cationic peptide originally purified from human blood ultrafiltrate. The native peptide contains two disulfide bonds and is unique regarding its primary structure. Its biological role is not known but a previous study showed that chemically synthesized L...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2009.10.006
更新日期:2010-01-01 00:00:00
abstract::Human dipeptidyl peptidase IV (hDPPIV, alternative name: CD26) inhibitors provide an effective strategy for the treatment of type 2 diabetes. Recently, our research group discovered a non substrate-mimic inhibitory dipeptide, Trp-Arg, by the systematic analysis of a dipeptide library. In the present study, a tripeptid...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2014.01.027
更新日期:2014-04-01 00:00:00
abstract::To investigate possible influences of growth hormone-releasing hormone (GHRH) and somatostatin (SRIF) on auditory perceptional processes, 12 subjects received either placebo (sodium chloride 0.9%), GHRH (50 micrograms), or SRIF (100 micrograms) on different days. Late auditory evoked potentials (AEP) were computed and...
journal_title:Peptides
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/0196-9781(95)00024-e
更新日期:1995-01-01 00:00:00
abstract::Intestinal levels of immunoreactive neurotensin (iNT) and neuromedin N (iNMN), as well as mRNA for the NT/NMN precursor, were elevated during the suckling period in rats. While transient expression of NT/NMN was observed at 1-5 days of age in the proximal small intestine and colon, NT/NMN levels in the ileum increased...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(93)90161-9
更新日期:1993-11-01 00:00:00
abstract::Studies were conducted in order to determine if selected neurons could be isolated from the brain using Sepharose-linked recognition complexes directed against or related to the biosynthetic/neurosecretory product of the desired neuronal population. Immunoreactive LRF neurons were precipitated when dispersed cells of ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(85)90061-0
更新日期:1985-03-01 00:00:00
abstract::In order to elucidate the relationship between secretory pathway and processing for precursor molecule of peptide hormones, we performed immunoelectron microscopic studies to localize POMC-derived peptides in mouse cultured L cells (fibroblasts without secretory granules) and in mouse AtT20 cells (ACTH secreting pitui...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/0196-9781(91)90092-4
更新日期:1991-05-01 00:00:00
abstract::alpha-MSH immunoreactive peptides were fractionated and characterized in rat and human brain and rat pituitary by reversed phase high pressure liquid chromatographic techniques. alpha-MSH and deacetylated alpha-MSH were two major naturally existing peptides in both brain and pituitary gland. Subsequent experiments exa...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(81)80126-x
更新日期:1981-10-01 00:00:00
abstract::Hypocretin-1 (hcrt-1) and hypocretin-2 (hcrt-2) are two recently discovered hypothalamic neuropeptides. In the present study, using double immunofluorescent techniques, the co-localization of hcrt-1 and hcrt-2 was examined in neuronal soma and fibers/terminals located, respectively, in the cat hypothalamus and brainst...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(02)00084-0
更新日期:2002-08-01 00:00:00
abstract::Vascular permeability and endothelial cell damage has been shown to occur in rats subjected to trauma with hemorrhagic-shock. Although the factors responsible for the endothelial cell injury are unknown, it has been hypothesized that toxic factors produced in response to hemorrhagic-shock originate in the gut and are ...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/j.peptides.2005.05.001
更新日期:2005-12-01 00:00:00
abstract::Large species differences have been previously observed in the pharmacology of bradykinin (BK) B2 receptor antagonists. We investigated the effect of two novel non-peptide antagonists, compound 9 (a benzodiazepine peptidomimetic related to icatibant) and the thiosemicarbazide bradyzide on the rabbit B2 receptor (contr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00481-8
更新日期:2001-09-01 00:00:00
abstract::A new, p -carborane containing analog of tyrosine, 3-[1-hydroxy-1,12-dicarba-closo-dodecaboran (12)-12-yl]alanine, was prepared from protected 3-[1-hydroxy-1,12-dicarba-closo-dodecaboran (12)-12-yl]propionic acid in five steps using Oppolzer's sultam methodology for asymmetric hydroxyamination as the key step. The tyr...
journal_title:Peptides
pub_type: 杂志文章
doi:10.1016/s0196-9781(01)00650-7
更新日期:2002-04-01 00:00:00