Abstract:
:Pseudomonas aeruginosa, a significant cause of human morbidity and mortality, uses a type 3 secretion system (T3SS) to inject effector toxins into host cells. We previously reported that P. aeruginosa uses ADP-ribosyltransferase (ADPr) activity of the T3SS effector ExoS for intracellular replication. T3SS translocon (ΔpopB)-mutants, which can export, but not translocate effectors across host membranes, retained intracellular replication. We hypothesized that secreted effectors mediate translocon-independent intracellular replication. Translocon mutants of PAO1 lacking one or more of its three known effectors (ExoS, ExoT and ExoY) were used. All translocon mutants, irrespective of effectors expressed, localized to intracellular vacuoles. Translocon-effector null mutants and translocon-exoS mutants showed defective intracellular replication. Mutants in exoT, exoY or both replicated as efficiently as translocon mutants expressing all effectors. Complementation of translocon-effector null mutants with native exoS or a membrane localization domain mutant of exoS, but not the ADPr mutant exoS (pUCPexoSE381D), restored intracellular replication, correlating with increased bacteria per vacuole. Thus, P. aeruginosa is capable of intravacuolar replication that requires ExoS ADPr activity, but not the translocon. These data suggest that T3SS effectors can participate in pathogenesis without translocon-mediated translocation across host membranes, and that intracellular bacteria can contribute to P. aeruginosa pathogenesis within epithelial cells.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Hritonenko V,Evans DJ,Fleiszig SMdoi
10.1016/j.micinf.2012.08.007subject
Has Abstractpub_date
2012-12-01 00:00:00pages
1366-73issue
15eissn
1286-4579issn
1769-714Xpii
S1286-4579(12)00217-1journal_volume
14pub_type
杂志文章abstract::The role of virus-derived small RNAs (vsRNAs) has been identified as an antiviral mechanism in plants, arthropods, and nematodes. Although mammalian DNA viruses have been observed to encode functional miRNAs, whether RNA virus infection generates functional vsRNAs remains under discussion. This article reviews the mos...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2005.09.016
更新日期:2006-03-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
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journal_title:Microbes and infection
pub_type: 杂志文章
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01656-8
更新日期:2002-10-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.02.001
更新日期:2012-07-01 00:00:00
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.05.009
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.12.002
更新日期:2007-03-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2011.06.001
更新日期:2011-11-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2017.04.001
更新日期:2017-06-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.02.019
更新日期:2006-06-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.01.009
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
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更新日期:1999-07-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2011.01.010
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.01.012
更新日期:2005-04-01 00:00:00
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journal_title:Microbes and infection
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2008.09.013
更新日期:2009-01-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
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更新日期:2020-04-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
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abstract::Infection of mice with Plasmodium berghei NK65 represents a well-recognized malaria model in which infection is accompanied by an intense hepatic inflammatory response. Enzyme-inducible nitric oxide synthase is an important regulator of inflammation and leukocyte recruitment in microvessels, but these functions have y...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2013.08.001
更新日期:2013-11-01 00:00:00
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journal_title:Microbes and infection
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