Quantitative polymerase chain reaction for Mycobacterium tuberculosis in so-called Eales' disease.

Abstract:

PURPOSE:To report mycobacterial load in the vitreous of patients labeled as having Eales' disease. METHODS:Eighty-eight patients were prospectively enrolled into 3 groups: 28 patients with so-called Eales' disease (group A); 30 positive controls with specific uveitis syndromes (group B), and 30 negative controls (group C). The undiluted vitreous humor samples were collected and subjected to real-time PCR assay for MPB64 gene of Mycobacterium tuberculosis (MTB) and load quantified. RESULTS:Sixteen (57.14%) vitreous fluid samples in group A; 1 sample in group B, and none of the samples in group C were positive for MTB genome from the vitreous. The copies of MTB genomes in the positive samples in group A were 1.52 × 10(4) to 1.01 × 10(6). CONCLUSION:MTB genome was demonstrated in more than 50% of vitreous fluid samples with significant bacillary load, indicating that half of patients with so-called Eales' disease are indeed cases of tubercular vasculitis.

journal_name

Ocul Immunol Inflamm

authors

Singh R,Toor P,Parchand S,Sharma K,Gupta V,Gupta A

doi

10.3109/09273948.2012.658134

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

153-7

issue

3

eissn

0927-3948

issn

1744-5078

journal_volume

20

pub_type

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