Use of PCR in endophthalmitis.

Abstract:

:In eyes with suspected endophthalmitis, early diagnosis and appropriate treatment have been noted to be associated with a better visual outcome. Currently, however, confirmation of the diagnosis of endophthalmitis (bacterial and/or fungal) is dependent on conventional techniques of microbiological isolation of organisms which require between one and twelve days. Furthermore, many samples prove to be culture-negative. In order to improve the rate of microbiological diagnosis, PCR technology has been successfully applied to the detection of bacteria and fungi in ocular samples. Specific oligonucleotide primers have been used to detect the presence of pathogens, which have been subsequently identified using RFLP analysis, DNA sequencing, and/or cloning techniques. Results demonstrated that PCR-based methods are rapidly able to confirm the presence of pathogens with high specificity and sensitivity. PCR-based techniques have also been used to rule out with confidence the presence of pathogens, a unique advantage of this methodology. The use of molecular methods has significantly increased the number of intraocular samples from which a confirmed diagnosis is made and reduced the time to laboratory diagnosis. PCR-based methods promise to be useful diagnostic tools in the management of these patients, especially those from whom ocular samples prove to be culture-negative.

journal_name

Ocul Immunol Inflamm

authors

Okhravi N,Adamson P,Lightman S

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

189-200

issue

3

eissn

0927-3948

issn

1744-5078

journal_volume

8

pub_type

杂志文章,评审
  • Optical Coherence Tomography Angiography in Uveitic Glaucoma - A Pilot Study.

    abstract::Purpose: To compare vessel density (VD) as measured by optical coherence tomography angiography (OCTA), in uveitic eyes with secondary glaucoma (UG) with VD in uveitic eyes without glaucoma (UV), eyes with primary open-angle glaucoma (POAG), and healthy eyes (HY). Methods: VD was measured at the following segmentation...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2020.1745246

    authors: Lommatzsch C,Bauermann P,Heimes-Bussmann B,Nolte C,Heinz C

    更新日期:2020-11-13 00:00:00

  • Causes of Hypertensive Anterior Uveitis in Thailand.

    abstract:PURPOSE:To determine the prevalence of viral infections in patients with hypertensive anterior uveitis in Thailand from polymerase chain reaction (PCR) of aqueous humor. METHODS:Thirty-one patients with anterior uveitis with intraocular pressure (IOP) above 25 mmHg were included for PCR analysis for cytomegalovirus (C...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2019.1678651

    authors: Khieu C,Kongyai N,Pathanapitoon K,Van Der Eijk AA,Rothova A

    更新日期:2020-05-18 00:00:00

  • Association between Skin Thickness Measurements with Corneal Biomechanical Properties and Dry Eye Tests in Systemic Sclerosis.

    abstract::Purpose: To analyse the association between skin score with corneal biomechanics and dry eye tests in systemic sclerosis (SSc). Methods: 112 cases (limited SSc (lcSSc), n=50; diffuse SSc  (dcSSc), n=32; controls, n=30) were included in the study. Corneal hysteresis (CH), corneal resistance factor (CRF), Schirmer 1, 2 ...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2018.1501496

    authors: Ayhan Z,Kaya M,Ozturk T,Arikan G,Birlik M

    更新日期:2019-01-01 00:00:00

  • Orbital sarcoidosis manifesting with enophthalmos.

    abstract:PURPOSE:To describe a case of orbital sarcoidosis manifesting with enophthalmos. METHODS:A 60-year-old woman presented with right enophthalmos. She had a palpable mass in her anterior orbit inferiorly. She underwent a complete ophthalmic evaluation, magnetic resonance imaging, and histopathologic examination. RESULTS...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273940601038589

    authors: Attia S,Zaouali S,Jeguirim H,Njim L,Kriaa S,Yahia SB,Khairallah M

    更新日期:2006-12-01 00:00:00

  • Tolerated cilium in the anterior chamber.

    abstract:PURPOSE:To describe a patient with a tolerated cilium in the anterior chamber. METHODS:A 15-year-old girl, referred with a 5-year trauma history, was examined. A cilium was noted in the anterior chamber without any inflammation during routine ophthalmological examination. RESULTS:The cilium was removed surgically and...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章,评审

    doi:10.1076/ocii.11.1.73.15576

    authors: Kargi SH,Oz O,Erdinc E,Teke MY,Firat E

    更新日期:2003-03-01 00:00:00

  • Retinal immunopathology in horses with uveitis.

    abstract:BACKGROUND:Equine uveitis is a spontaneous disorder of horses that can serve as a model for the study of human uveitis. Although the initial presentation is that of an anterior uveitis, retinal involvement has been noted in some cases. We report here the immunohistopathology of retinas from horses with uveitis. METHOD...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1076/ocii.6.4.239.4026

    authors: Kalsow CM,Dwyer AE

    更新日期:1998-12-01 00:00:00

  • Treatment of severe ocular hypotony in AIDS patients with cytomegalovirus retinitis and cidofovir-associated uveitis.

    abstract:OBJECTIVE:To describe the medical treatment of severe ocular hypotony in HIV-seropositive patients with cytomegalovirus retinitis and cidofovir-associated uveitis. PATIENTS AND METHODS:Two HIV-seropositive patients with cytomegalovirus retinitis and cidofovir-associated uveitis complicated by severe ocular hypotony we...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1076/ocii.9.3.211.3968

    authors: Accorinti M,Ciapparoni V,Pirraglia MP,Pivetti-Pezzi P

    更新日期:2001-09-01 00:00:00

  • Fluorodeoxyglucose positron emission tomography for the diagnosis of sarcoidosis in patients with unexplained chronic uveitis.

    abstract:PURPOSE:To assess the value of fluorine-18 fluorodeoxyglucose positron emission tomography ((18)F-FDG PET) in patients with unexplained chronic uveitis. METHODS:Retrospective review of 19 (18)F-FDG PET scans. All patients had normal chest CT. In the final analysis, the authors used criteria similar to the criteria of ...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273940902788221

    authors: Seve P,Billotey C,Janier M,Grange JD,Broussolle C,Kodjikian L

    更新日期:2009-05-01 00:00:00

  • Atypical presentation of multiple evanescent white dot syndrome (MEWDS).

    abstract:PURPOSE:To present fundus autofluorescence (FAF), indocyanine green angiography (ICGA), and microperimetry (MP) findings of a patient with multiple evanescent white dot syndrome (MEWDS). METHODS:Observational case report. RESULTS:A 30-year-old woman with blurry vision was referred for evaluation. Fundus examination r...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273940802026060

    authors: Yenerel NM,Kucumen B,Gorgun E,Dinc UA

    更新日期:2008-05-01 00:00:00

  • Virus-Induced Anterior Uveitis (VIAU) in Immunocompromised Patients.

    abstract:PURPOSE:To describe the clinical characteristics, diagnosis, and treatment of VIAU in immunocompromised patients. METHODS:A critical review of literature was performed. RESULTS:Diagnosis and treatment of VIAU in immunocompromised patients may be a challenge due to atypical clinical-courses, severe presentations, and ...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章,评审

    doi:10.1080/09273948.2018.1483519

    authors: de-la-Torre A

    更新日期:2018-01-01 00:00:00

  • Clinical Course and Outcomes of Pediatric Tubercular Uveitis in North India.

    abstract:PURPOSE:To analyze the clinical features, course, management, and outcomes of tubercular (TB) uveitis in the pediatric population and assess the response to anti-tubercular therapy (ATT). METHODS:Hospital records of children (≤16 years) from a large tertiary-care institute between January 2001 and December 2015 were r...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2017.1296579

    authors: Kaur S,Aggarwal K,Agarwal A,Suri D,Gupta A,Singh R,Bansal R,Singh S,Singh N,Gupta A,Gupta V

    更新日期:2018-01-01 00:00:00

  • Is There a True Neuroretinitis in Idiopathic Retinal Vasculitis, Aneurysms, and Neuroretinitis (IRVAN) Syndrome ?

    abstract::Purpose : To clarify whether a true neuroretinitis is part of  idiopathic retinal vasculitis, aneurysms, and neuroretinitis (IRVAN) syndrome.Methods : Critical literature review.Results : Neuroretinitis has been considered as a prominent feature of IRVAN syndrome. It has been described as late diffuse optic  disc stai...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2020.1836234

    authors: Khairallah M,Khochtali S,Ksiaa I

    更新日期:2020-12-17 00:00:00

  • More than a masquerade syndrome: atypical presentations of vitreoretinal lymphomas.

    abstract:PURPOSE:To present a population of patients sharing atypical manifestations of vitreoretinal lymphoma (VRL). METHODS:Institutional case series in a single tertiary center. Patients with cytologically proven VRL, referred between November 2009 and May 2010, were retrospectively reviewed. Diagnosis of VRL was based on c...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273948.2013.835427

    authors: AlQahtani A,Touitou V,Cassoux N,Aknin C,Merle-Beral H,Bodaghi B,LeHoang P

    更新日期:2014-06-01 00:00:00

  • Effect of prolonged oral acyclovir treatment in acute retinal necrosis.

    abstract:BACKGROUND:To examine the effectiveness of prolonged oral acyclovir treatment in patients with acute retinal necrosis (ARN). METHODS:Fifty-five ARN patients (62 eyes) who had been treated with short-term or long-term oral acyclovir were identified in this retrospective study: one group treated with prolonged oral acyc...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273948.2012.689073

    authors: Jeon S,Kakizaki H,Lee WK,Jee D

    更新日期:2012-08-01 00:00:00

  • Clinical application of a homogeneous colorimetric assay for tear lysozyme.

    abstract:PURPOSE:Tear lysozyme and tear lactoferrin are enzymes synthesized by the lacrimal gland. Their concentration in human tears reflects tear gland function. Tear gland dysfunction can lead to ocular surface disease. We developed a colorimetric lysozyme assay. The objective of this study was to determine the diagnostic po...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1076/ocii.7.1.7.8108

    authors: Klaeger AJ,Cevallos V,Sherman MD,Whitcher JP,Stephens RS

    更新日期:1999-03-01 00:00:00

  • Ocular Behçet's Disease: Changing Patterns Over Time, Complications and Long-Term Visual Prognosis.

    abstract:PURPOSE:To investigate demographic, clinical features and complications of Italian patients with ocular Behçet's disease (BD) over time. METHODS:Retrospective study of 385 patients examined from 1968 to 2011, 265 of whom had follow-up ≥ 12 months. RESULTS:A significant increase in the proportion of females (p = 0.03)...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273948.2015.1094095

    authors: Accorinti M,Pesci FR,Pirraglia MP,Abicca I,Pivetti-Pezzi P

    更新日期:2017-02-01 00:00:00

  • Anti-Inflammatory Effect of Dehydroxymethylepoxyquinomicin, a Nuclear factor-κB Inhibitor, on Endotoxin-Induced Uveitis in Rats In vivo and In vitro.

    abstract::Purpose: To determine the anti-inflammatory effects of dehydroxymethylepoxyquinomicin (DHMEQ), a nuclear factor-κB (NF-κB) inhibitor, on endotoxin-induced uveitis (EIU) in rats.Methods: EIU was induced by a subcutaneous injection of lipopolysaccharide (LPS) in Lewis rats. DHMEQ was injected intraperitoneally concurren...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2019.1568502

    authors: Ando Y,Keino H,Kudo A,Hirakata A,Okada AA,Umezawa K

    更新日期:2020-01-01 00:00:00

  • Semicircular tumor of the iris and uveitis as unilocal manifestation of sarcoidosis.

    abstract:PURPOSE:To describe an unusual manifestation of sarcoidosis as a large tumor of the iris and ciliary body without any other involvement of the body. METHODS:We describe a 20-year-old female presenting with a granulomatous tumor of the right iris and ciliary body and concomitant uveitis. RESULTS:Extensive ocular and s...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/092739490500156

    authors: Maca SM,Firbas U,Hauff W,Zettinig G,Horvat R,Barisani-Asenbauer T

    更新日期:2004-09-01 00:00:00

  • Intravitreal Adalimumab for the Control of Breakthrough Intraocular Inflammation.

    abstract:PURPOSE:Investigate the efficacy of intravitreal adalimumab in breakthrough panuveitis in patients on systemic adalimumab for more than 3 months. METHODS:Retrospective study of patients on systemic adalimumab with breakthrough panuveitis requiring intravitreal adalimumab therapy. RESULTS:Seven eyes of four patients w...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2017.1335756

    authors: Kheir WJ,Mehanna CJ,Abdul Fattah M,Al Ghadban S,El Sabban M,Mansour AM,Hamam RN

    更新日期:2018-01-01 00:00:00

  • Chronic Anterior Uveitis Associated with Relapsing Polychondritis: A Case Report.

    abstract::Purpose: To report a case of chronic anterior uveitis associated with relapsing polychondritis (RP).Case Report: A 43-year-old female patient presenting with bilateral progressive vision loss was diagnosed with bilateral anterior uveitis. According to her clinical signs and symptoms and biopsy results, RP was ultimate...

    journal_title:Ocular immunology and inflammation

    pub_type: 信件

    doi:10.1080/09273948.2018.1547834

    authors: Wei Y,Chi Y,Fang Y,Wu D,Qiao R

    更新日期:2020-01-01 00:00:00

  • The Emerging Challenge of Diagnosing Drug-resistant Tubercular Uveitis: Experience of 110 Eyes from North India.

    abstract::Background: Rapid and timely diagnosis of tubercular uveitis (TBU) is of paramount importance to save these eyes from blindness. The present study was, therefore, undertaken to carry out a comparative evaluation of Gene Xpert MTB/RIF (Xpert), MTBDRplus and Multiplex PCR (MPCR) for the diagnosis of TBU. These tests wer...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2019.1655581

    authors: Sharma K,Gupta A,Sharma M,Sharma A,Bansal R,Sharma SP,Singh RD,Gupta V

    更新日期:2019-10-03 00:00:00

  • Characteristics of immunotolerance induced by intratesticular injection of S-antigen.

    abstract::Experimental autoimmune uveoretinitis (EAU) induced by immunization of male Lewis rats with bovine S-antigen (S-Ag) is prevented by injection of S-Ag into the testis prior to immunization. This protection of animals is due to the induction of systemic immunotolerance or immunosuppression, which the authors designate o...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273949509085041

    authors: Ren J,Singh AK,Shichi H

    更新日期:1995-01-01 00:00:00

  • Multimodal Imaging in Ocular Tuberculosis.

    abstract::Intraocular tuberculosis presents a diagnostic challenge because of its protean clinical manifestations. Thus, it becomes imperative to distinguish chorioretinal inflammation due to tuberculosis from other infectious as well as non-infectious posterior uveitis since the clinical manifestations of these entities may be...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章,评审

    doi:10.1080/09273948.2016.1231332

    authors: Agarwal A,Mahajan S,Khairallah M,Mahendradas P,Gupta A,Gupta V

    更新日期:2017-02-01 00:00:00

  • Association Study of ARMC9 Gene Variants with Vogt-Koyanagi-Harada Disease in Japanese Patients.

    abstract::Purpose: To investigate whether variants in the ARMC9 gene encoding KU-MEL-1 are associated with Vogt-Koyanagi-Harada (VKH) disease in a Japanese population. Methods: We recruited 380 Japanese patients with VKH disease and 744 Japanese healthy controls to genotype seven single-nucleotide polymorphisms (SNPs) in ARMC9....

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2018.1523438

    authors: Ohno T,Meguro A,Takeuchi M,Yamane T,Teshigawara T,Kitaichi N,Horie Y,Namba K,Ohno S,Nakao K,Sakamoto T,Sakai T,Nakano T,Keino H,Okada AA,Takeda A,Fukuhara T,Mashimo H,Ohguro N,Oono S,Enaida H,Okinami S,Mizuki

    更新日期:2019-01-01 00:00:00

  • Nedocromil sodium and sodium cromoglycate inhibit plasma extravasation in the guinea-pig conjunctiva.

    abstract::Nedocromil sodium (NS) and sodium cromoglycate (CS) (both applied locally into the conjunctival sac) reduced in a dose dependent manner plasma extravasation induced by topical bradykinin and by topical substance P (SP), NS being approximately ten times more potent than CS. Plasma extravasation induced by SP was unaffe...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273949509057809

    authors: Javdan P,Figini M,Ricciardolo FL,Geppetti P

    更新日期:1995-01-01 00:00:00

  • Endophthalmitis in HIV Infection.

    abstract::Purpose: The role of HIV infection in exogenous and endogenous endophthalmitis has not been clarified. We aim to assess the potential role of HIV as a risk factor or a poor prognostic feature in this sight-threatening condition. Methods: Literature review. Review of evidence: Evidence for endophthalmitis in HIV patien...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1080/09273948.2019.1699580

    authors: Tsirouki T,Steffen J,Dastiridou A,Praidou A,Androudi S

    更新日期:2020-10-02 00:00:00

  • Systemic immune response to Acanthamoeba keratitis in the Chinese hamster.

    abstract::Recrudescence is a common and troubling feature of Acanthamoeba keratitis and suggests that corneal infection with this organism fails to stimulate the systemic immune apparatus. The present study examined the cell-mediated and humoral immune responses to Acanthamoeba keratitis in the Chinese hamster. Corneal infectio...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273949709085064

    authors: Van Klink F,Leher H,Jager MJ,Alizadeh H,Taylor W,Niederkorn JY

    更新日期:1997-12-01 00:00:00

  • Uveitis related to juvenile idiopathic arthritis: familial cases and possible genetic implication in the pathogenesis.

    abstract:PURPOSE:To describe four cases of familial Juvenile Idiopathic Arthritis (JIA) associated uveitis. DESIGN:Retrospective observational case series. METHODS:Clinical data from patients who present familial JIA-associated uveitis are described and possible genetic influence in disease etiology is reviewed. RESULTS:Two ...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.3109/09273941003678837

    authors: Julián K,Terrada C,Quartier P,Lehoang P,Bodaghi B

    更新日期:2010-06-01 00:00:00

  • Uveitis referral pattern in a Midwest University Eye Center.

    abstract::We determined the referral pattern and visual prognosis for patients with uveitis at a tertiary university eye center in the Midwestern U.S. over a five-year period. A retrospective chart review was conducted on 853 patients seen in the Department of Ophthalmology and Visual Sciences (DOVS) at Washington University an...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章

    doi:10.1076/ocii.11.4.287.18270

    authors: Oruc S,Kaplan AD,Galen M,Kaplan HJ

    更新日期:2003-12-01 00:00:00

  • Cytomegalovirus: virological facts for clinicians.

    abstract::Human cytomegalovirus (HCMV) is a complex DNA virus encoding more than 200 viral proteins. This highly adapted opportunist agent has developed several ways to evade the immune system. Among all clinical features due to HCMV, retinitis occurs especially in severely immunosuppressed patients, particularly during the end...

    journal_title:Ocular immunology and inflammation

    pub_type: 杂志文章,评审

    doi:10.1076/ocii.7.3.133.3998

    authors: Bodaghi B,Michelson S

    更新日期:1999-12-01 00:00:00