Abstract:
:Stiffness and cytokine in blood levels show 24-h rhythms in rheumatoid arthritis (RA) patients. We previously revealed that higher therapeutic effects were obtained in RA patients and RA model animals when the dosing time of methotrexate was chosen according to the 24-h rhythms to cytokine. In this study, we examined whether a dosing time-dependency of the therapeutic effect of tacrolimus (TAC) could be detected in collagen-induced arthritis (CIA) and MRL/lpr mice. To measure the levels of cytokines and serum amyloid A (SAA), blood was collected from CIA mice at different times. TAC was administered at two different dosing times based on these findings and its effects on arthritis and toxicity were examined. Plasma tumor necrosis factor (TNF)-α, interleukin-6 (IL-6), and SAA concentrations showed obvious 24-h rhythms with higher levels during the light phase and lower levels during the dark phase after RA crisis. The arthritis score and leukocyte counts were significantly lower in the group treated at 2 h after the light was turned on (HALO) than in the control and 14 HALO-treated groups. Our findings suggest that choosing an optimal dosing time could lead to the effective treatment of RA by TAC.
journal_name
J Pharmacol Scijournal_title
Journal of pharmacological sciencesauthors
Obayashi K,Tomonari M,Yoshimatsu H,Fukuyama R,Ieiri I,Higuchi S,To Hdoi
10.1254/jphs.11029fpsubject
Has Abstractpub_date
2011-01-01 00:00:00pages
264-73issue
3eissn
1347-8613issn
1347-8648pii
JST.JSTAGE/jphs/11029FPjournal_volume
116pub_type
杂志文章abstract::P-glycoprotein (P-gp)-induced drug resistance is a major road block for successful cancer chemotherapy. Through phenotypic screening, the compound 2-(2-chlorophenylimino)-5-(4-dimethylaminobenzylidene) thiazolidin-4-one (CDBT) was discovered to have potent anti-tumor activity in P-gp over-expressing drug-resistant non...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.14050fp
更新日期:2014-01-01 00:00:00
abstract::Rapid-scanning cofocal microscopy has been applied to the analysis of early phase Ca(2+) transients in ventricular and atrial cardiomyocytes. On electrical stimulation of ventricular myocytes, Ca(2+) concentration begins to rise earliest at the Z-line level and becomes uniform throughout the cytoplasm within about 10 ...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.93.248
更新日期:2003-11-01 00:00:00
abstract::B cells are important in the development of autoimmune disorders through mechanisms involving dysregulated polyclonal B-cell activation, production of pathogenic antibodies, and targeting which reduces inflammation and tissue damage effectively but often leads to patients suffering from secondary infection. Paeoniflor...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2015.02.011
更新日期:2015-05-01 00:00:00
abstract::Incomplete recovery of myocardial contraction after reperfusion following brief ischemia is called the "stunning phenomenon" in an animal experiment. A hydrophilic 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor (statin) does not affect this phenomenon, but lipophilic statins further reduce the con...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.08017fp
更新日期:2008-04-01 00:00:00
abstract:OBJECTIVE:Treatment with the chemotherapeutic agent, doxorubicin (DOX), is limited by side effects. We have previously demonstrated that fasudil, a Rho/ROCK inhibitor, has antioxidant, anti-inflammatory and anti-apoptotic effects in contrast-induced acute kidney injury model. The present study to investigated the possi...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2020.10.002
更新日期:2021-01-01 00:00:00
abstract::The pharmacological effects of rivoglitazone, a novel thiazolidinedione-derivative peroxisome proliferator-activated receptor (PPAR)-gamma agonist, were characterized in vitro and in vivo. Rivoglitazone activated human PPARgamma more potently compared with rosiglitazone and pioglitazone and had little effect on PPARal...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.09084fp
更新日期:2009-10-01 00:00:00
abstract::Metabolic syndrome (MetS) induces serious complications; therefore, we developed a noninvasive MetS model using an extremely small minipig, the Microminipig. For 8 weeks, Microminipigs were administrated a high-fat and high-cholesterol diet (HFCD) for atherosclerosis and N(G)-nitro-l-arginine methyl ester (l-NAME) for...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.14171sc
更新日期:2014-01-01 00:00:00
abstract::Effects of V(1) (OPC-21268) and V(2) (OPC-31260) vasopressin antagonists on blood pressure (BP) short-term variability were investigated in adult spontaneously hypertensive rats (SHR) under basal conditions and after the stimulation of vasopressin release by hemorrhage. BP was recorded intra-arterially and sampled at ...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.95.47
更新日期:2004-05-01 00:00:00
abstract::Calf Spleen Extractive Injection (CSEI), a small peptides enriched extraction, performs immunomodulatory activity on cancer patients suffering from radiotherapy or chemotherapy. The present study aims to investigate the anti-hepatocellular carcinoma effects of CSEI in cells and tumor-xenografted mouse models. In HepG2...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2016.08.006
更新日期:2016-10-01 00:00:00
abstract::Melittin is a major peptide component of sweet bee venom that possesses anti-allergic, anti-inflammatory, anti-arthritis, anti-cancer, and neuroprotective properties. However, the therapeutic effects of melittin on muscle injury have not been elucidated. We investigated the therapeutic effects of melittin on muscle in...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2019.03.009
更新日期:2019-05-01 00:00:00
abstract::Chotosan (CTS), a traditional herbal formula called Kampo medicine, was shown to be effective in the treatment of vascular dementia in a clinical study, and exerted protective effects against transient cerebral ischemia-induced cognitive impairment in mice. In the present study, we investigated the neuroprotective eff...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2017.10.009
更新日期:2017-11-01 00:00:00
abstract::Intravital multiphoton microscopy has opened a new era in the field of biological imaging. Focal excitation of fluorophores by simultaneous attack of multiple (normally "two") photons generates images with high spatial resolution, and use of near-infrared lasers for multiphoton excitation allows penetration of thicker...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.12r03cp
更新日期:2012-01-01 00:00:00
abstract::Our previous study showed that organobismuth compounds induce apoptosis in human promyelocytic leukemia cells, although solid tumor cell lines were relatively resistant. Herein, we investigated the primary cellular target of these compounds in HeLa cells. One organobismuth compound, bi-chlorodibenzo[c,f][1,5]thiabismo...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.09020fp
更新日期:2009-04-01 00:00:00
abstract::It has been widely accepted that macrophages are divided into M1 "pro-inflammatory" macrophages and M2 "anti-inflammatory" macrophages and an uncontrolled macrophage polarization plays an important role in the pathogenesis of different diseases. As the main substance of total glucosides of peony, paeoniflorin (PF), ha...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2015.12.007
更新日期:2016-03-01 00:00:00
abstract::We have shown that coadministration of sulpiride and fluvoxamine preferentially increases the release of dopamine in the prefrontal cortex. To study the possible role of the cortical cholinergic system in this effect, we combined several other antipsychotic drugs with fluvoxamine and examined the effects on acetylchol...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.sc0060187
更新日期:2006-12-01 00:00:00
abstract::N-methyl-N-nitrosourea (MNU) is widely used to study the mechanism of retinal degenerative diseases (RDs) because of its selectivity of photoreceptor cell death. Many reports suggest that excessive nitric oxide (NO) plays a crucial role in neuronal cell death. We hypothesized that nitric oxide synthase (NOS)/NO are in...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2015.02.008
更新日期:2015-03-01 00:00:00
abstract::RhoA upregulation has been suggested in bronchial smooth muscles (BSMs) of asthmatic rats. Here, we cloned/characterized the 5'-promoter region of the rat rhoA. A transcription-initiation site was identified at 66-bp upstream of the reference sequence, GenBank-BC061732. Luciferase assay using interleukin-13 (IL-13)-st...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.09346sc
更新日期:2010-01-01 00:00:00
abstract::Mithramycin A (MTM) has been shown to inhibit cancer growth by blocking the binding of Sp-family transcription factors to gene regulatory elements and is used for the treatment of leukemia and testicular cancer in the United States. In contrast, MTM has also been shown to exert neuroprotective effects in normal cells....
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.13r02cp
更新日期:2013-01-01 00:00:00
abstract::The hepatitis C virus (HCV) is an enveloped virus with a single positive-strand RNA genome of about 9.6 kb. It is a major cause of liver disease worldwide. Clear understanding of the viral life cycle has been hampered by the lack of a robust cell culture system. While the development of the HCV replicon system was a m...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.fm0070040
更新日期:2007-10-01 00:00:00
abstract::Overexpression of constitutively active (CA)-G alpha13 significantly increased the expression of interleukin (IL)-1beta and IL-6 mRNAs and proteins in rat cardiac fibroblasts. IL-1beta mRNA induction by CA-G alpha13 was suppressed by diphenyleneiodonium (DPI), an NADPH oxidase inhibitor, but not by BAPTA-AM, an intrac...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.fp0051036
更新日期:2006-06-01 00:00:00
abstract::The biological basis for the therapeutic mechanisms of depression are still unknown. While performing EST (expressed sequence tag) analysis to identify some molecular machinery responsible for the antidepressant effect, we determined the full-length nucleotide sequence of rat frizzled-3 protein (Frz3) cDNA. Interestin...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.fp0050461
更新日期:2005-11-01 00:00:00
abstract::Alzheimer's disease (AD) is the most common neurodegenerative disorder of late life characterized by insidious, chronic, and progressive memory impairment in association with the accumulation of senile plaques, neurofibrillary tangles, and massive loss of neurons. Apoptosis is believed to be an important contributor t...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1254/jphs.cpj04006x
更新日期:2005-03-01 00:00:00
abstract::Probucol has antioxidant effects and inhibits inflammation. Farnesoid X receptor (FXR) is a nuclear receptor that regulates autophagy, which is regarded as the key cause of the activation of hepatic stellate cell (HSC). In this study, the effects of probucol on HSC activation and autophagy in vitro and vivo and the ro...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2018.12.005
更新日期:2019-02-01 00:00:00
abstract::Despite of the huge socio-economic burden, stroke still represents an unmet therapeutic need. Researchers failed to reproduce preclinical efficacy in subsequent clinical development. To bridge this translation failure, the Stroke Therapy Academic Industry Round Table (STAIR) has suggested a rigorous, robust, and detai...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.08246fp
更新日期:2009-03-01 00:00:00
abstract::The role of extracellular calcium in the interaction between intracellular cAMP and nitric oxide (NO)/cGMP on the contractility of rat diaphragm pretreated with cumulative concentrations of aminophylline (0.36 - 3.60 mM) was investigated. In a Ca2+-free medium, NG-nitro-L-arginine methyl ester (L-NAME) (1 and 3 mM) de...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.sce04001x
更新日期:2004-12-01 00:00:00
abstract::Multi-glycoside, one of the extracted compounds from Tripterygium wilfordii HOOK. f. (GTW), has been shown to be clinically effective in suppressing glomerular inflammation in chronic kidney disease. However, its clinical application is often limited by its cytotoxic actions on the liver. This study was performed to c...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.11157fp
更新日期:2012-01-01 00:00:00
abstract::Since induced pluripotent stem cells (iPSCs) were generated from mice and humans by Professor Shinya Yamanaka et al. in 2006 and 2007, respectively, a variety of human-derived cells have been generated, including myocardial, liver, retinal pigment epithelial, and neuronal cells. These iPSCs are now used not only in cl...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章,评审
doi:10.1016/j.jphs.2019.06.002
更新日期:2019-08-01 00:00:00
abstract::Bee venom (BV) has been used in patients with rheumatoid arthritis, a condition characterized by rheumatoid joint destruction mediated, in large part, by matrix metalloproteinases (MMPs). We investigated the effects of melittin, a major component of bee venom, on the production of MMPs in human rheumatoid arthritic fi...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.sc0070215
更新日期:2008-01-01 00:00:00
abstract::This study was undertaken to identify novel approaches to pharmacological treatment of asthma. Here we hypothesize that the platelet-activating factor receptor antagonist ginkgolide B (GB) in combination with the antioxidant carotenoid astaxanthin (ASX) suppresses T cell activation comparably to two commonly-used anti...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1254/jphs.94.129
更新日期:2004-02-01 00:00:00
abstract::Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal lung disease characterized by inflammation, multifocal fibrotic lesions and excessive collagen deposition with limited therapies. As a major bioactive compound in garlic, S-allyl-l-cysteine (SAC) is a neuroprotective drug candidate to prevent cognitive de...
journal_title:Journal of pharmacological sciences
pub_type: 杂志文章
doi:10.1016/j.jphs.2019.03.002
更新日期:2019-04-01 00:00:00