Abstract:
:Intravital multiphoton microscopy has opened a new era in the field of biological imaging. Focal excitation of fluorophores by simultaneous attack of multiple (normally "two") photons generates images with high spatial resolution, and use of near-infrared lasers for multiphoton excitation allows penetration of thicker specimens, enabling biologists to visualize living cellular dynamics deep inside tissues and organs without thin sectioning. Moreover, the minimized photo-bleaching and toxicity associated with multiphoton techniques is beneficial for imaging of live specimens for extended observation periods. Here we focus on recent findings using intravital multiphoton imaging of dynamic biological systems such as the immune system and bone homeostasis. The immune system comprises highly dynamic networks, in which many cell types actively travel throughout the body and interact with each other in specific areas. Therefore, real-time intravital imaging represents a powerful tool for understanding the mechanisms underlying this dynamic system.
journal_name
J Pharmacol Scijournal_title
Journal of pharmacological sciencesauthors
Kikuta J,Ishii Mdoi
10.1254/jphs.12r03cpsubject
Has Abstractpub_date
2012-01-01 00:00:00pages
193-7issue
3eissn
1347-8613issn
1347-8648pii
DN/JST.JSTAGE/jphs/12R03CPjournal_volume
119pub_type
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