Analysis of ammonia monooxygenase and archaeal 16S rRNA gene fragments in nitrifying acid-sulfate soil microcosms.

abstract：:Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Ki...

journal_title：Microbes and environments

authors： Evans JS,López-Legentil S,Erwin PM

更新日期：2018-12-28 00:00:00

abstract：:The inhibitory effects of ammonia on two different degradation pathways of methanogenic acetate were evaluated using a pure culture (Methanosaeta thermophila strain PT) and defined co-culture (Methanothermobacter thermautotrophicus strain TM and Thermacetogenium phaeum strain PB), which represented aceticlastic and sy...

journal_title：Microbes and environments

authors： Kato S,Sasaki K,Watanabe K,Yumoto I,Kamagata Y

更新日期：2014-01-01 00:00:00

abstract：:Rice seedlings were inoculated with Azospirillum sp. B510 and transplanted into a paddy field. Growth in terms of tiller numbers and shoot length was significantly increased by inoculation. Principal-coordinates analysis of rice bacterial communities using the 16S rRNA gene showed no overall change from B510 inoculati...

journal_title：Microbes and environments

authors： Bao Z,Sasaki K,Okubo T,Ikeda S,Anda M,Hanzawa E,Kakizaki K,Sato T,Mitsui H,Minamisawa K

更新日期：2013-01-01 00:00:00

abstract：:Members of the Legionella genus find suitable conditions for their growth and survival in nuclear power plant cooling circuits. To limit the proliferation of Legionella pathogenic bacteria in nuclear power plant cooling circuits, and ensure that levels remain below regulatory thresholds, monochloramine treatment can b...

journal_title：Microbes and environments

authors： Jakubek D,Guillaume C,Binet M,Leblon G,DuBow M,Le Brun M

更新日期：2013-01-01 00:00:00

abstract：:We investigated the effects of the water status (flooded or non-flooded) and presence of the nosZ gene in bradyrhizobia on the bradyrhizobial community structure in a factorial experiment that examined three temperature levels (20°C, 25°C, and 30°C) and two soil types (andosol and gray lowland soil) using microcosm in...

journal_title：Microbes and environments

authors： Saeki Y,Nakamura M,Mason MLT,Yano T,Shiro S,Sameshima-Saito R,Itakura M,Minamisawa K,Yamamoto A

更新日期：2017-06-24 00:00:00

abstract：:The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanis...

journal_title：Microbes and environments

authors： Nonaka L,Maruyama F,Miyamoto M,Miyakoshi M,Kurokawa K,Masuda M

更新日期：2012-01-01 00:00:00

abstract：:HcRNAV is a small icosahedral virus that infects the shellfish-killing marine dinoflagellate Heterocapsa circularisquama, which harbors a dicistronic linear single-stranded RNA (ssRNA) genome ca. 4.4 kb in length. Its major capsid protein (MCP) gene sequence is not expressed by various strains of Escherichia coli, pos...

journal_title：Microbes and environments

authors： Wada K,Kimura K,Hasegawa A,Fukuyama K,Nagasaki K

更新日期：2012-01-01 00:00:00

abstract：:The screening of pollutant degraders by relying solely on cultivation techniques such as liquid enrichment often fails to isolate the actual degraders in the environment. Community analyses by PCR-denaturing gradient gel electrophoresis (DGGE) were performed to isolate bacteria that can degrade 3-chlorobenzoate (3CB) ...

journal_title：Microbes and environments

authors： Morimoto S,Ogawa N,Hasebe A,Fujii T

更新日期：2008-01-01 00:00:00

abstract：:Elevated concentrations of atmospheric CO2 ([CO2]) enhance the production and emission of methane in paddy fields. In the present study, the effects of elevated [CO2], elevated temperature (ET), and no nitrogen fertilization (LN) on methanogenic archaeal and methane-oxidizing bacterial community structures in a free-a...

journal_title：Microbes and environments

authors： Liu D,Tago K,Hayatsu M,Tokida T,Sakai H,Nakamura H,Usui Y,Hasegawa T,Asakawa S

更新日期：2016-09-29 00:00:00

abstract：:Marine fungus-like eukaryotic unicellular protists (thraustochytrids) are considered to play an important role in the marine microbial food web. However, their abundance, distribution, and relative biomass in coastal waters have not yet been examined in detail. By using a flow cytometry method (FCM) for the rapid enum...

journal_title：Microbes and environments

authors： Duan Y,Sen B,Xie N,Paterson JS,Chen Z,Wang G

更新日期：2018-07-04 00:00:00

abstract：:The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are specific to bacteria....

journal_title：Microbes and environments

authors： Ikenaga M,Sakai M

更新日期：2014-09-17 00:00:00

abstract：:Rapid and continuous pressure treatment was realized using a hydraulic pump and the momentary decompression following high pressurization was used to inactivate bacteria. The number of colony-forming E. coli decreased to 1/1000 in response to 10 cycles of pressure treatment. In groundwater samples, repeated pressure t...

journal_title：Microbes and environments

authors： Baba T,Yamaguchi N,Tani K,Nasu M

更新日期：2011-01-01 00:00:00

abstract：:Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite red...

journal_title：Microbes and environments

authors： Kobayashi S,Hira D,Yoshida K,Toyofuku M,Shida Y,Ogasawara W,Yamaguchi T,Araki N,Oshiki M

更新日期：2018-12-28 00:00:00

abstract：:Antibiotics in animal husbandry are used to maintain welfare, but lead to the generation of resistant strains. We analyzed commensal multidrug-resistant Escherichia coli from pigs at the beginning and end of the production cycle in a farm with a farrow-to-finish system in order to investigate whether clonal spread or ...

journal_title：Microbes and environments

authors： Mazurek J,Bok E,Baldy-Chudzik K

更新日期：2018-09-29 00:00:00

abstract：:Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae...

journal_title：Microbes and environments

authors： Ootsuka M,Nishizawa T,Hasegawa M,Kurusu Y,Ohta H

更新日期：2018-12-28 00:00:00

abstract：:A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types of full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 bacterial 16...

journal_title：Microbes and environments

authors： Narihiro T,Terada T,Kikuchi K,Iguchi A,Ikeda M,Yamauchi T,Shiraishi K,Kamagata Y,Nakamura K,Sekiguchi Y

更新日期：2009-01-01 00:00:00

abstract：:N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signaling molecules by many Gram-negative bacteria. Here, 413 bacterial strains were obtained from the roots of potato plants and screened for AHL-degrading bacteria using Chromobacterium violaceum reporter strains. Sixty one isolates degraded N-hexanoyl-L-ho...

journal_title：Microbes and environments

authors： Rashid R,Morohoshi T,Someya N,Ikeda T

更新日期：2011-01-01 00:00:00

abstract：:Denitrifiers can produce and consume nitrous oxide (N(2)O). While little N(2)O is emitted from rice paddy soil, the same soil produces N(2)O when the land is drained and used for upland crop cultivation. In this study, we collected soils from two types of fields each at three locations in Japan; one type of field had ...

journal_title：Microbes and environments

authors： Tago K,Ishii S,Nishizawa T,Otsuka S,Senoo K

更新日期：2011-01-01 00:00:00

abstract：:Over the past few decades, anammox bacteria have been recognized as key players that contribute significantly to the release of large amounts of nitrogen in the global marine nitrogen cycle. In the present study, the diversity, community composition, and abundance of anammox bacteria from the sediments of four diverse...

journal_title：Microbes and environments

authors： Shehzad A,Liu J,Yu M,Qismat S,Liu J,Zhang XH

更新日期：2016-06-25 00:00:00

abstract：:The effects of a nitrogen-fixing tree, black locust (Robinia pseudoacacia), on the distribution of bacterial species were examined in a Japanese black pine (Pinus thunbergii) and black locust-dominated area. DNA was extracted from the soil at depths of 0-5 and 5-10 cm, collected at the border between a Japanese black ...

journal_title：Microbes and environments

authors： Taniguchi T,Kataoka R,Tamai S,Yamanaka N,Futai K

更新日期：2009-01-01 00:00:00

abstract：:We investigated biofilm formation on biodegradable plastics in freshwater samples. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) was covered by a biofilm after an incubation in freshwater samples. A next generation sequencing analysis of the bacterial communities of biofilms that formed on PHBH films revealed t...

journal_title：Microbes and environments

authors： Morohoshi T,Oi T,Aiso H,Suzuki T,Okura T,Sato S

更新日期：2018-09-29 00:00:00

abstract：:The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the ...

journal_title：Microbes and environments

authors： Chien MF,Tosa S,Huang CC,Endo G

更新日期：2009-01-01 00:00:00

abstract：:Small cyanobacteria (<2 µm, pico-cyanobacteria) are abundant in waters deeper than the oxic-anoxic zone in the halocline of a saline meromictic lake, Lake Suigetsu, Fukui, Japan. We have isolated 101 strains that were grouped into six groups by means of the phycobiliprotein composition and sequence homology of the int...

journal_title：Microbes and environments

authors： Ohki K,Yamada K,Kamiya M,Yoshikawa S

更新日期：2012-01-01 00:00:00

abstract：:In order to understand the relationships between understory bamboo and soil properties, we compared microbial community structures in the soil of a Betula ermanii boreal forest with Sasa kurilensis present and removed using high-throughput DNA sequencing. The presence of understory S. kurilensis strongly affected soil...

journal_title：Microbes and environments

authors： Kong B,Chen L,Kasahara Y,Sumida A,Ono K,Wild J,Nagatake A,Hatano R,Hara T

更新日期：2017-06-24 00:00:00

abstract：:The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. ...

journal_title：Microbes and environments

authors： Wang Y,Shi T,Huang G,Gong J

更新日期：2018-09-29 00:00:00

abstract：:The relative isolation and unique physical properties of the Savusavu Hot Springs in Fiji may yield unique thermophiles. This study was conducted to determine the presence of aerobic thermophilic bacteria in these hot springs. A total of 104 thermophilic bacterial isolates were characterized and using Thermus and Baci...

journal_title：Microbes and environments

authors： Narayan VV,Hatha MA,Morgan HW,Rao D

更新日期：2008-01-01 00:00:00

abstract：:Chemosynthetic microbial communities develop and form dense cell aggregates in slightly alkaline sulfidic hot springs in the temperature range of 70-86°C at Nakabusa, Japan. Nitrogenase activity has recently been detected in the microbial communities collected. To identify possible members capable of nitrogen fixation...

journal_title：Microbes and environments

authors： Nishihara A,Thiel V,Matsuura K,McGlynn SE,Haruta S

更新日期：2018-12-28 00:00:00

abstract：:Due to their obligate symbiotic nature and lack of long-term storage methods, the strain collection of arbuscular mycorrhizal (AM) fungi requires periodic proliferation using a pot culture with host plants. Therefore, a method to evaluate the purity of proliferated AM fungal cultures is critical for the quality contro...

journal_title：Microbes and environments

authors： Ohtomo R,Oka N,Morimoto S

更新日期：2019-12-27 00:00:00

abstract：:Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 typ...

journal_title：Microbes and environments

authors： Ciesielska A,Bohacz J,Korniłłowicz-Kowalska T,Stączek P

更新日期：2014-01-01 00:00:00

abstract：:Giant viruses of 'Megaviridae' have the ability to widely disperse around the globe. We herein examined 'Megaviridae' communities in four distinct aquatic environments (coastal and offshore seawater, brackish water, and hot spring freshwater), which are distantly located from each other (between 74 and 1,765 km), usin...

journal_title：Microbes and environments

authors： Li Y,Endo H,Gotoh Y,Watai H,Ogawa N,Blanc-Mathieu R,Yoshida T,Ogata H

更新日期：2019-09-25 00:00:00