Abstract:
:Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite reductase (NirK) from Nitrososphaera viennensis and investigated its enzymatic properties. The recombinant protein catalyzed the reduction of 15NO2- to 15NO, the oxidation of hydroxylamine (15NH2OH) to 15NO, and the production of 14-15N2O from 15NH2OH and 14NO2-. To the best of our knowledge, the present study is the first to document the enzymatic properties of AOA NirK.
journal_name
Microbes Environjournal_title
Microbes and environmentsauthors
Kobayashi S,Hira D,Yoshida K,Toyofuku M,Shida Y,Ogasawara W,Yamaguchi T,Araki N,Oshiki Mdoi
10.1264/jsme2.ME18058subject
Has Abstractpub_date
2018-12-28 00:00:00pages
428-434issue
4eissn
1342-6311issn
1347-4405journal_volume
33pub_type
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