Flow Cytometry for Rapid Enumeration and Biomass Quantification of Thraustochytrids in Coastal Seawaters.

abstract：:Atrazine is a triazine herbicide that is widely used to control broadleaf weeds. Its widespread use over the last 50 years has led to the potential contamination of soils, groundwater, rivers, and lakes. Its main route of complete degradation is via biological means, which is carried out by soil microbiota using a 6-s...

journal_title：Microbes and environments

authors： Fernandes AFT,Wang P,Staley C,Aparecida Silva Moretto J,Miguel Altarugio L,Chagas Campanharo S,Guedes Stehling E,Jay Sadowsky M

更新日期：2020-01-01 00:00:00

abstract：:Denitrifiers can produce and consume nitrous oxide (N(2)O). While little N(2)O is emitted from rice paddy soil, the same soil produces N(2)O when the land is drained and used for upland crop cultivation. In this study, we collected soils from two types of fields each at three locations in Japan; one type of field had ...

journal_title：Microbes and environments

authors： Tago K,Ishii S,Nishizawa T,Otsuka S,Senoo K

更新日期：2011-01-01 00:00:00

abstract：:Fucose-containing oligosaccharides on the cell surface of some pathogenic bacteria are thought to be important for host-microbe interactions and to play a major role in the pathogenicity of bacterial pathogens. Here, we screened marine bacteria for glycosyltransferases using two methods: a one-pot glycosyltransferase ...

journal_title：Microbes and environments

authors： Kajiwara H,Toda M,Mine T,Nakada H,Yamamoto T

更新日期：2012-01-01 00:00:00

abstract：:The genome of Streptomyces scabies, the predominant causal agent of potato common scab, encodes a potential cutinase, the protein Sub1, which was previously shown to be specifically induced in the presence of suberin. The sub1 gene was expressed in Escherichia coli and the recombinant protein Sub1 was purified and cha...

journal_title：Microbes and environments

authors： Jabloune R,Khalil M,Ben Moussa IE,Simao-Beaunoir AM,Lerat S,Brzezinski R,Beaulieu C

更新日期：2020-01-01 00:00:00

abstract：:The addition of ferrihydrite to methanogenic microbial communities obtained from a thermophilic anaerobic digester suppressed methanogenesis in a dose-dependent manner. The amount of reducing equivalents consumed by the reduction of iron was significantly smaller than that expected from the decrease in the production ...

journal_title：Microbes and environments

authors： Yamada C,Kato S,Ueno Y,Ishii M,Igarashi Y

更新日期：2014-01-01 00:00:00

abstract：:The simultaneous extraction of plant organelle (mitochondria and plastid) genes during the DNA extraction step is a major limitation in investigating the community structures of bacteria associated with plants because organelle SSU rRNA genes are easily amplified by PCR using primer sets that are specific to bacteria....

journal_title：Microbes and environments

authors： Ikenaga M,Sakai M

更新日期：2014-09-17 00:00:00

abstract：:The nifV gene encodes homocitrate synthase, the enzyme that catalyzes the formation of homocitrate, which is essential for arranging the FeMo-cofactor in the catalytic center of nitrogenase. Some host plants, such as Lotus japonicus, supply homocitrate to their symbionts, in this case, Mesorhizobium loti, which lacks ...

journal_title：Microbes and environments

authors： Hashimoto S,Wongdee J,Songwattana P,Greetatorn T,Goto K,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2019-12-27 00:00:00

abstract：:The mining industry generates huge amounts of wastewater, containing toxic heavy metals. Treatment to remove heavy metals is necessary and recent work has been focused on finding more environmentally friendly materials for removing heavy metals from wastewater. Biosorption can be an effective process for heavy metal r...

journal_title：Microbes and environments

authors： Mohamad OA,Hao X,Xie P,Hatab S,Lin Y,Wei G

更新日期：2012-01-01 00:00:00

abstract：:Rapid and continuous pressure treatment was realized using a hydraulic pump and the momentary decompression following high pressurization was used to inactivate bacteria. The number of colony-forming E. coli decreased to 1/1000 in response to 10 cycles of pressure treatment. In groundwater samples, repeated pressure t...

journal_title：Microbes and environments

authors： Baba T,Yamaguchi N,Tani K,Nasu M

更新日期：2011-01-01 00:00:00

abstract：:Small cyanobacteria (<2 µm, pico-cyanobacteria) are abundant in waters deeper than the oxic-anoxic zone in the halocline of a saline meromictic lake, Lake Suigetsu, Fukui, Japan. We have isolated 101 strains that were grouped into six groups by means of the phycobiliprotein composition and sequence homology of the int...

journal_title：Microbes and environments

authors： Ohki K,Yamada K,Kamiya M,Yoshikawa S

更新日期：2012-01-01 00:00:00

abstract：:Recent studies demonstrated that phylogenetically more diverse and abundant bacteria and fungi than previously considered are responsible for denitrification in terrestrial environments. We herein examined the effects of land-use types on the community composition of those denitrifying microbes based on their nitrite ...

journal_title：Microbes and environments

authors： Fujimura R,Azegami Y,Wei W,Kakuta H,Shiratori Y,Ohte N,Senoo K,Otsuka S,Isobe K

更新日期：2020-01-01 00:00:00

abstract：:We established artificial consortia of bacteria inoculated from soil and a green alga, Chlorella vulgaris NIES-227. The bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. The community of bacteria formed in the consorti...

journal_title：Microbes and environments

authors： Ueda H,Otsuka S,Senoo K

更新日期：2010-01-01 00:00:00

abstract：:Rice seedlings were inoculated with Azospirillum sp. B510 and transplanted into a paddy field. Growth in terms of tiller numbers and shoot length was significantly increased by inoculation. Principal-coordinates analysis of rice bacterial communities using the 16S rRNA gene showed no overall change from B510 inoculati...

journal_title：Microbes and environments

authors： Bao Z,Sasaki K,Okubo T,Ikeda S,Anda M,Hanzawa E,Kakizaki K,Sato T,Mitsui H,Minamisawa K

更新日期：2013-01-01 00:00:00

abstract：:A cellular approach combining Direct Viable Counting and Fluorescent In Situ Hybridization using a one-step multiple-probe technique and Solid Phase Cytometry (DVC-FISH-SPC) was developed to monitor total viable vibrios and cover the detection of a large diversity of vibrios. FISH combined three probes in the same ass...

journal_title：Microbes and environments

authors： Girard L,Peuchet S,Servais P,Henry A,Charni-Ben-Tabassi N,Baudart J

更新日期：2017-09-27 00:00:00

abstract：:MicroRNAs (miRNAs) are a group of small non-coding RNAs that suppress the expression of target mRNAs. The seed sequence of miRNA plays a crucial role in recognizing the 3'-untranslated region of the target mRNA. Cells infected with a simian foamy virus (SFV) isolated from an African green monkey (Chlorocebus aethiops)...

journal_title：Microbes and environments

authors： Hashimoto-Gotoh A,Kitao K,Miyazawa T

更新日期：2020-01-01 00:00:00

abstract：:The present study describes the occurrence of a unique archaeal ammonia monooxygenase alpha subunit (amoA) gene in nitrifying acid-sulfate soil microcosms at pH 3.5. The soil was collected from an abandoned paddy field in Thailand. Microcosms were incubated in the dark at 30°C for 372 days with the following three tre...

journal_title：Microbes and environments

authors： Nakaya A,Onodera Y,Nakagawa T,Satoh K,Takahashi R,Sasaki S,Tokuyama T

更新日期：2009-01-01 00:00:00

abstract：:A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. In...

journal_title：Microbes and environments

authors： Kongpol A,Kato J,Tajima T,Vangnai AS

更新日期：2012-01-01 00:00:00

abstract：:The Gram-negative marine propylene-assimilating bacterium, strain PE-TB08W, was isolated from surface seawater. A structural gene analysis using the 16S rRNA gene showed 96, 94, and 95% similarities to Halioglobus species, Haliea sp. ETY-M, and Haliea sp. ETY-NAG, respectively. A phylogenetic tree analysis showed that...

journal_title：Microbes and environments

authors： Suzuki T,Yazawa T,Morishita N,Maruyama A,Fuse H

更新日期：2019-03-30 00:00:00

abstract：:We examined the colonization rate and communities of arbuscular mycorrhizal fungi (AMF) in the roots of Pyrus pyrifolia var. culta (Japanese pear) in orchards to investigate the effect of phosphorus (P) fertilization on AMF. Soil cores containing the roots of Japanese pear were collected from 13 orchards in Tottori Pr...

journal_title：Microbes and environments

authors： Yoshimura Y,Ido A,Iwase K,Matsumoto T,Yamato M

更新日期：2013-01-01 00:00:00

abstract：:The effects of a nitrogen-fixing tree, black locust (Robinia pseudoacacia), on the distribution of bacterial species were examined in a Japanese black pine (Pinus thunbergii) and black locust-dominated area. DNA was extracted from the soil at depths of 0-5 and 5-10 cm, collected at the border between a Japanese black ...

journal_title：Microbes and environments

authors： Taniguchi T,Kataoka R,Tamai S,Yamanaka N,Futai K

更新日期：2009-01-01 00:00:00

abstract：:To clarify altitudinal changes in the bacterial community on Gulkana Glacier in Alaska, we analyzed bacterial 16S rRNA gene by low-cycle PCR amplification, denaturing gradient gel electrophoresis (DGGE), and culturing in a snowmelt medium at 4°C. Low-cycle PCR-based cloning revealed the presence of 100 bacterial OTUs;...

journal_title：Microbes and environments

authors： Segawa T,Takeuchi N,Ushida K,Kanda H,Kohshima S

更新日期：2010-01-01 00:00:00

abstract：:Termites depend nutritionally on their gut microbes, and protistan, bacterial, and archaeal gut communities have been extensively studied. However, limited information is available on viruses in the termite gut. We herein report the complete genome sequence (99,517 bp) of a phage obtained during a genome analysis of "...

journal_title：Microbes and environments

authors： Pramono AK,Kuwahara H,Itoh T,Toyoda A,Yamada A,Hongoh Y

更新日期：2017-06-24 00:00:00

abstract：:We investigated biofilm formation on biodegradable plastics in freshwater samples. Poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBH) was covered by a biofilm after an incubation in freshwater samples. A next generation sequencing analysis of the bacterial communities of biofilms that formed on PHBH films revealed t...

journal_title：Microbes and environments

authors： Morohoshi T,Oi T,Aiso H,Suzuki T,Okura T,Sato S

更新日期：2018-09-29 00:00:00

abstract：:Soybean plants host endosymbiotic dinitrogen (N2)-fixing bacteria from the genus Bradyrhizobium. Under oxygen-limiting conditions, Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum perform denitrification by sequentially reducing nitrate (NO3-) to nitrous oxide (N2O) or N2. The anaerobic reduction of NO3- to ...

journal_title：Microbes and environments

authors： Siqueira AF,Sugawara M,Arashida H,Minamisawa K,Sánchez C

更新日期：2020-01-01 00:00:00

abstract：:The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanis...

journal_title：Microbes and environments

authors： Nonaka L,Maruyama F,Miyamoto M,Miyakoshi M,Kurokawa K,Masuda M

更新日期：2012-01-01 00:00:00

abstract：:The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a pr...

journal_title：Microbes and environments

authors： Tago K,Itoh H,Kikuchi Y,Hori T,Sato Y,Nagayama A,Okubo T,Navarro R,Aoyagi T,Hayashi K,Hayatsu M

更新日期：2014-01-01 00:00:00

abstract：:The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxi...

journal_title：Microbes and environments

authors： Yanagawa K,Tani A,Yamamoto N,Hachikubo A,Kano A,Matsumoto R,Suzuki Y

更新日期：2016-06-25 00:00:00

abstract：:Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 typ...

journal_title：Microbes and environments

authors： Ciesielska A,Bohacz J,Korniłłowicz-Kowalska T,Stączek P

更新日期：2014-01-01 00:00:00

abstract：:The relative isolation and unique physical properties of the Savusavu Hot Springs in Fiji may yield unique thermophiles. This study was conducted to determine the presence of aerobic thermophilic bacteria in these hot springs. A total of 104 thermophilic bacterial isolates were characterized and using Thermus and Baci...

journal_title：Microbes and environments

authors： Narayan VV,Hatha MA,Morgan HW,Rao D

更新日期：2008-01-01 00:00:00

abstract：:N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signaling molecules by many Gram-negative bacteria. Here, 413 bacterial strains were obtained from the roots of potato plants and screened for AHL-degrading bacteria using Chromobacterium violaceum reporter strains. Sixty one isolates degraded N-hexanoyl-L-ho...

journal_title：Microbes and environments

authors： Rashid R,Morohoshi T,Someya N,Ikeda T

更新日期：2011-01-01 00:00:00