Establishment of a bacterial expression system and immunoassay platform for the major capsid protein of HcRNAV, a dinoflagellate-infecting RNA virus.

abstract：:A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types of full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 bacterial 16...

journal_title：Microbes and environments

authors： Narihiro T,Terada T,Kikuchi K,Iguchi A,Ikeda M,Yamauchi T,Shiraishi K,Kamagata Y,Nakamura K,Sekiguchi Y

更新日期：2009-01-01 00:00:00

abstract：:N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signaling molecules by many Gram-negative bacteria. Here, 413 bacterial strains were obtained from the roots of potato plants and screened for AHL-degrading bacteria using Chromobacterium violaceum reporter strains. Sixty one isolates degraded N-hexanoyl-L-ho...

journal_title：Microbes and environments

authors： Rashid R,Morohoshi T,Someya N,Ikeda T

更新日期：2011-01-01 00:00:00

abstract：:The inhibitory effects of ammonia on two different degradation pathways of methanogenic acetate were evaluated using a pure culture (Methanosaeta thermophila strain PT) and defined co-culture (Methanothermobacter thermautotrophicus strain TM and Thermacetogenium phaeum strain PB), which represented aceticlastic and sy...

journal_title：Microbes and environments

authors： Kato S,Sasaki K,Watanabe K,Yumoto I,Kamagata Y

更新日期：2014-01-01 00:00:00

abstract：:Small cyanobacteria (<2 µm, pico-cyanobacteria) are abundant in waters deeper than the oxic-anoxic zone in the halocline of a saline meromictic lake, Lake Suigetsu, Fukui, Japan. We have isolated 101 strains that were grouped into six groups by means of the phycobiliprotein composition and sequence homology of the int...

journal_title：Microbes and environments

authors： Ohki K,Yamada K,Kamiya M,Yoshikawa S

更新日期：2012-01-01 00:00:00

abstract：:Fucose-containing oligosaccharides on the cell surface of some pathogenic bacteria are thought to be important for host-microbe interactions and to play a major role in the pathogenicity of bacterial pathogens. Here, we screened marine bacteria for glycosyltransferases using two methods: a one-pot glycosyltransferase ...

journal_title：Microbes and environments

authors： Kajiwara H,Toda M,Mine T,Nakada H,Yamamoto T

更新日期：2012-01-01 00:00:00

abstract：:Four hundred and fifty nine isolates of fluorescent pseudomonads were obtained from the leaves and roots of potato plants. Of these, 20 leaf isolates and 28 root isolates induced violacein production in two N-acylhomoserine lactone (AHL)-reporter strains-Chromobacterium violaceum CV026 and VIR24. VIR24 is a new report...

journal_title：Microbes and environments

authors： Someya N,Morohoshi T,Okano N,Otsu E,Usuki K,Sayama M,Sekiguchi H,Ikeda T,Ishida S

更新日期：2009-01-01 00:00:00

abstract：:Chitin amendment is an agricultural management strategy for controlling soil-borne plant disease. We previously reported an exponential decrease in chitin added to incubated upland soil. We herein investigated the transition of the bacterial community structure in chitin-degrading soil samples over time and the charac...

journal_title：Microbes and environments

authors： Iwasaki Y,Ichino T,Saito A

更新日期：2020-01-01 00:00:00

abstract：:Nitrogen (N) cycles have been directly linked to the functional stability of ecosystems because N is an essential element for life. Furthermore, the supply of N to organisms regulates primary productivity in many natural ecosystems. Microbial communities have been shown to significantly contribute to N cycles because ...

journal_title：Microbes and environments

authors： Isobe K,Ohte N

更新日期：2014-01-01 00:00:00

abstract：:Marine fungus-like eukaryotic unicellular protists (thraustochytrids) are considered to play an important role in the marine microbial food web. However, their abundance, distribution, and relative biomass in coastal waters have not yet been examined in detail. By using a flow cytometry method (FCM) for the rapid enum...

journal_title：Microbes and environments

authors： Duan Y,Sen B,Xie N,Paterson JS,Chen Z,Wang G

更新日期：2018-07-04 00:00:00

abstract：:Diazotrophs had not previously been identified among bacterial species in the phylum Bacteroidetes until the rapid expansion of bacterial genome sequences, which revealed the presence of nitrogen fixation (nif) genes in this phylum. We herein determined the draft genome sequences of Bacteroides graminisolvens JCM 1509...

journal_title：Microbes and environments

authors： Inoue J,Oshima K,Suda W,Sakamoto M,Iino T,Noda S,Hongoh Y,Hattori M,Ohkuma M

更新日期：2015-01-01 00:00:00

abstract：:Members of the Legionella genus find suitable conditions for their growth and survival in nuclear power plant cooling circuits. To limit the proliferation of Legionella pathogenic bacteria in nuclear power plant cooling circuits, and ensure that levels remain below regulatory thresholds, monochloramine treatment can b...

journal_title：Microbes and environments

authors： Jakubek D,Guillaume C,Binet M,Leblon G,DuBow M,Le Brun M

更新日期：2013-01-01 00:00:00

abstract：:The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxi...

journal_title：Microbes and environments

authors： Yanagawa K,Tani A,Yamamoto N,Hachikubo A,Kano A,Matsumoto R,Suzuki Y

更新日期：2016-06-25 00:00:00

abstract：:Ultra-small microorganisms are ubiquitous in Earth's environments. Ultramicrobacteria, which are defined as having a cell volume of <0.1 μm3, are often numerically dominant in aqueous environments. Cultivated representatives among these bacteria, such as members of the marine SAR11 clade (e.g., "Candidatus Pelagibacte...

journal_title：Microbes and environments

authors： Nakai R

更新日期：2020-01-01 00:00:00

abstract：:A wide variety of haloorganic compounds undergo reductive dehalogenation by certain anaerobic microorganisms. Metabolic reductive dehalogenation is coupled with energy-conserving respiratory electron transport in which a halogenated compound is used as the terminal electron acceptor, the biological process called deha...

journal_title：Microbes and environments

authors： Hiraishi A

更新日期：2008-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a group of small non-coding RNAs that suppress the expression of target mRNAs. The seed sequence of miRNA plays a crucial role in recognizing the 3'-untranslated region of the target mRNA. Cells infected with a simian foamy virus (SFV) isolated from an African green monkey (Chlorocebus aethiops)...

journal_title：Microbes and environments

authors： Hashimoto-Gotoh A,Kitao K,Miyazawa T

更新日期：2020-01-01 00:00:00

abstract：:Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae...

journal_title：Microbes and environments

authors： Ootsuka M,Nishizawa T,Hasegawa M,Kurusu Y,Ohta H

更新日期：2018-12-28 00:00:00

abstract：:Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Ki...

journal_title：Microbes and environments

authors： Evans JS,López-Legentil S,Erwin PM

更新日期：2018-12-28 00:00:00

abstract：:We examined the colonization rate and communities of arbuscular mycorrhizal fungi (AMF) in the roots of Pyrus pyrifolia var. culta (Japanese pear) in orchards to investigate the effect of phosphorus (P) fertilization on AMF. Soil cores containing the roots of Japanese pear were collected from 13 orchards in Tottori Pr...

journal_title：Microbes and environments

authors： Yoshimura Y,Ido A,Iwase K,Matsumoto T,Yamato M

更新日期：2013-01-01 00:00:00

abstract：:In order to understand the relationships between understory bamboo and soil properties, we compared microbial community structures in the soil of a Betula ermanii boreal forest with Sasa kurilensis present and removed using high-throughput DNA sequencing. The presence of understory S. kurilensis strongly affected soil...

journal_title：Microbes and environments

authors： Kong B,Chen L,Kasahara Y,Sumida A,Ono K,Wild J,Nagatake A,Hatano R,Hara T

更新日期：2017-06-24 00:00:00

abstract：:The genome of Streptomyces scabies, the predominant causal agent of potato common scab, encodes a potential cutinase, the protein Sub1, which was previously shown to be specifically induced in the presence of suberin. The sub1 gene was expressed in Escherichia coli and the recombinant protein Sub1 was purified and cha...

journal_title：Microbes and environments

authors： Jabloune R,Khalil M,Ben Moussa IE,Simao-Beaunoir AM,Lerat S,Brzezinski R,Beaulieu C

更新日期：2020-01-01 00:00:00

abstract：:Chemosynthetic microbial communities develop and form dense cell aggregates in slightly alkaline sulfidic hot springs in the temperature range of 70-86°C at Nakabusa, Japan. Nitrogenase activity has recently been detected in the microbial communities collected. To identify possible members capable of nitrogen fixation...

journal_title：Microbes and environments

authors： Nishihara A,Thiel V,Matsuura K,McGlynn SE,Haruta S

更新日期：2018-12-28 00:00:00

abstract：:The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a pr...

journal_title：Microbes and environments

authors： Tago K,Itoh H,Kikuchi Y,Hori T,Sato Y,Nagayama A,Okubo T,Navarro R,Aoyagi T,Hayashi K,Hayatsu M

更新日期：2014-01-01 00:00:00

abstract：:Rice seedlings were inoculated with Azospirillum sp. B510 and transplanted into a paddy field. Growth in terms of tiller numbers and shoot length was significantly increased by inoculation. Principal-coordinates analysis of rice bacterial communities using the 16S rRNA gene showed no overall change from B510 inoculati...

journal_title：Microbes and environments

authors： Bao Z,Sasaki K,Okubo T,Ikeda S,Anda M,Hanzawa E,Kakizaki K,Sato T,Mitsui H,Minamisawa K

更新日期：2013-01-01 00:00:00

abstract：:The Gram-negative marine propylene-assimilating bacterium, strain PE-TB08W, was isolated from surface seawater. A structural gene analysis using the 16S rRNA gene showed 96, 94, and 95% similarities to Halioglobus species, Haliea sp. ETY-M, and Haliea sp. ETY-NAG, respectively. A phylogenetic tree analysis showed that...

journal_title：Microbes and environments

authors： Suzuki T,Yazawa T,Morishita N,Maruyama A,Fuse H

更新日期：2019-03-30 00:00:00

abstract：:The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. ...

journal_title：Microbes and environments

authors： Wang Y,Shi T,Huang G,Gong J

更新日期：2018-09-29 00:00:00

abstract：:Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite red...

journal_title：Microbes and environments

authors： Kobayashi S,Hira D,Yoshida K,Toyofuku M,Shida Y,Ogasawara W,Yamaguchi T,Araki N,Oshiki M

更新日期：2018-12-28 00:00:00

abstract：:Arsenic (As) is a widely distributed toxic element in the environment and microorganisms have developed resistance mechanisms in order to tolerate it. The cellular response of the chemoorganotrophic arsenite (As[III])-oxidizing α-Proteobacteria, Sinorhizobium sp. strain A2, to arsenic was examined in the present study...

journal_title：Microbes and environments

authors： Fukushima K,Huang H,Hamamura N

更新日期：2015-01-01 00:00:00

abstract：:The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanis...

journal_title：Microbes and environments

authors： Nonaka L,Maruyama F,Miyamoto M,Miyakoshi M,Kurokawa K,Masuda M

更新日期：2012-01-01 00:00:00

abstract：:The nifV gene encodes homocitrate synthase, the enzyme that catalyzes the formation of homocitrate, which is essential for arranging the FeMo-cofactor in the catalytic center of nitrogenase. Some host plants, such as Lotus japonicus, supply homocitrate to their symbionts, in this case, Mesorhizobium loti, which lacks ...

journal_title：Microbes and environments

authors： Hashimoto S,Wongdee J,Songwattana P,Greetatorn T,Goto K,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2019-12-27 00:00:00

abstract：:Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 typ...

journal_title：Microbes and environments

authors： Ciesielska A,Bohacz J,Korniłłowicz-Kowalska T,Stączek P

更新日期：2014-01-01 00:00:00