Isolation of effective 3-chlorobenzoate-degraders in soil using community analyses by PCR-DGGE.

abstract：:Soil and rhizosphere environments were examined in order to determine the identity and relative abundance of bacteria that catabolize d- and l-amino acids as the sole source of carbon and nitrogen. All substrates were readily catabolized by bacteria from both environments, with most d-amino acids giving similar CFU co...

journal_title：Microbes and environments

authors： Radkov AD,McNeill K,Uda K,Moe LA

更新日期：2016-06-25 00:00:00

abstract：:The nifV gene encodes homocitrate synthase, the enzyme that catalyzes the formation of homocitrate, which is essential for arranging the FeMo-cofactor in the catalytic center of nitrogenase. Some host plants, such as Lotus japonicus, supply homocitrate to their symbionts, in this case, Mesorhizobium loti, which lacks ...

journal_title：Microbes and environments

authors： Hashimoto S,Wongdee J,Songwattana P,Greetatorn T,Goto K,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2019-12-27 00:00:00

abstract：:A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. In...

journal_title：Microbes and environments

authors： Kongpol A,Kato J,Tajima T,Vangnai AS

更新日期：2012-01-01 00:00:00

abstract：:Ultra-small microorganisms are ubiquitous in Earth's environments. Ultramicrobacteria, which are defined as having a cell volume of <0.1 μm3, are often numerically dominant in aqueous environments. Cultivated representatives among these bacteria, such as members of the marine SAR11 clade (e.g., "Candidatus Pelagibacte...

journal_title：Microbes and environments

authors： Nakai R

更新日期：2020-01-01 00:00:00

abstract：:Arsenic (As) is a widely distributed toxic element in the environment and microorganisms have developed resistance mechanisms in order to tolerate it. The cellular response of the chemoorganotrophic arsenite (As[III])-oxidizing α-Proteobacteria, Sinorhizobium sp. strain A2, to arsenic was examined in the present study...

journal_title：Microbes and environments

authors： Fukushima K,Huang H,Hamamura N

更新日期：2015-01-01 00:00:00

abstract：:Antibiotics in animal husbandry are used to maintain welfare, but lead to the generation of resistant strains. We analyzed commensal multidrug-resistant Escherichia coli from pigs at the beginning and end of the production cycle in a farm with a farrow-to-finish system in order to investigate whether clonal spread or ...

journal_title：Microbes and environments

authors： Mazurek J,Bok E,Baldy-Chudzik K

更新日期：2018-09-29 00:00:00

abstract：:Marine fungus-like eukaryotic unicellular protists (thraustochytrids) are considered to play an important role in the marine microbial food web. However, their abundance, distribution, and relative biomass in coastal waters have not yet been examined in detail. By using a flow cytometry method (FCM) for the rapid enum...

journal_title：Microbes and environments

authors： Duan Y,Sen B,Xie N,Paterson JS,Chen Z,Wang G

更新日期：2018-07-04 00:00:00

abstract：:Changes in the bacterial community in soil-water suspensions during the enrichment period of 2,4-D and 2,4,5-T degraders were examined using denaturing gradient gel electrophoresis (DGGE) analysis of the 16S rRNA gene. The nucleotide sequences of almost all major bands at the degradation of 2,4-D and 2,4,5-T correspon...

journal_title：Microbes and environments

authors： Huong NL,Itoh K,Suyama K

更新日期：2008-01-01 00:00:00

abstract：:Members of the Marseilleviridae family are large DNA viruses with icosahedral particle structures that infect Acanthamoeba cells. The first Marseillevirus to be discovered was isolated in 2009. Since then, several other members of the Marseilleviridae family have been reported, including Lausannevirus, Senegalvirus, C...

journal_title：Microbes and environments

authors： Takemura M

更新日期：2016-12-23 00:00:00

abstract：:Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 typ...

journal_title：Microbes and environments

authors： Ciesielska A,Bohacz J,Korniłłowicz-Kowalska T,Stączek P

更新日期：2014-01-01 00:00:00

abstract：:Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still con...

journal_title：Microbes and environments

authors： Urashima Y,Sonoda T,Fujita Y,Uragami A

更新日期：2012-01-01 00:00:00

abstract：:Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2‰ salinity) in arid...

journal_title：Microbes and environments

authors： Tang X,Xie G,Shao K,Dai J,Chen Y,Xu Q,Gao G

更新日期：2015-01-01 00:00:00

abstract：:Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite red...

journal_title：Microbes and environments

authors： Kobayashi S,Hira D,Yoshida K,Toyofuku M,Shida Y,Ogasawara W,Yamaguchi T,Araki N,Oshiki M

更新日期：2018-12-28 00:00:00

abstract：:The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanis...

journal_title：Microbes and environments

authors： Nonaka L,Maruyama F,Miyamoto M,Miyakoshi M,Kurokawa K,Masuda M

更新日期：2012-01-01 00:00:00

abstract：:Elevated concentrations of atmospheric CO2 ([CO2]) enhance the production and emission of methane in paddy fields. In the present study, the effects of elevated [CO2], elevated temperature (ET), and no nitrogen fertilization (LN) on methanogenic archaeal and methane-oxidizing bacterial community structures in a free-a...

journal_title：Microbes and environments

authors： Liu D,Tago K,Hayatsu M,Tokida T,Sakai H,Nakamura H,Usui Y,Hasegawa T,Asakawa S

更新日期：2016-09-29 00:00:00

abstract：:To clarify altitudinal changes in the bacterial community on Gulkana Glacier in Alaska, we analyzed bacterial 16S rRNA gene by low-cycle PCR amplification, denaturing gradient gel electrophoresis (DGGE), and culturing in a snowmelt medium at 4°C. Low-cycle PCR-based cloning revealed the presence of 100 bacterial OTUs;...

journal_title：Microbes and environments

authors： Segawa T,Takeuchi N,Ushida K,Kanda H,Kohshima S

更新日期：2010-01-01 00:00:00

abstract：:Rapid and continuous pressure treatment was realized using a hydraulic pump and the momentary decompression following high pressurization was used to inactivate bacteria. The number of colony-forming E. coli decreased to 1/1000 in response to 10 cycles of pressure treatment. In groundwater samples, repeated pressure t...

journal_title：Microbes and environments

authors： Baba T,Yamaguchi N,Tani K,Nasu M

更新日期：2011-01-01 00:00:00

abstract：:Terrestrial hydrocarbon seeps are an important source of naturally emitted methane over geological time. The exact community compositions responsible for carbon cycling beneath these surface features remain obscure. As sulfate reduction represents an essential process for anoxic organic mineralization, this study coll...

journal_title：Microbes and environments

authors： Cheng TW,Lin LH,Lin YT,Song SR,Wang PL

更新日期：2014-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a group of small non-coding RNAs that suppress the expression of target mRNAs. The seed sequence of miRNA plays a crucial role in recognizing the 3'-untranslated region of the target mRNA. Cells infected with a simian foamy virus (SFV) isolated from an African green monkey (Chlorocebus aethiops)...

journal_title：Microbes and environments

authors： Hashimoto-Gotoh A,Kitao K,Miyazawa T

更新日期：2020-01-01 00:00:00

abstract：:Four hundred and fifty nine isolates of fluorescent pseudomonads were obtained from the leaves and roots of potato plants. Of these, 20 leaf isolates and 28 root isolates induced violacein production in two N-acylhomoserine lactone (AHL)-reporter strains-Chromobacterium violaceum CV026 and VIR24. VIR24 is a new report...

journal_title：Microbes and environments

authors： Someya N,Morohoshi T,Okano N,Otsu E,Usuki K,Sayama M,Sekiguchi H,Ikeda T,Ishida S

更新日期：2009-01-01 00:00:00

abstract：:A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types of full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 bacterial 16...

journal_title：Microbes and environments

authors： Narihiro T,Terada T,Kikuchi K,Iguchi A,Ikeda M,Yamauchi T,Shiraishi K,Kamagata Y,Nakamura K,Sekiguchi Y

更新日期：2009-01-01 00:00:00

abstract：:Atrazine is a triazine herbicide that is widely used to control broadleaf weeds. Its widespread use over the last 50 years has led to the potential contamination of soils, groundwater, rivers, and lakes. Its main route of complete degradation is via biological means, which is carried out by soil microbiota using a 6-s...

journal_title：Microbes and environments

authors： Fernandes AFT,Wang P,Staley C,Aparecida Silva Moretto J,Miguel Altarugio L,Chagas Campanharo S,Guedes Stehling E,Jay Sadowsky M

更新日期：2020-01-01 00:00:00

abstract：:Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains a...

journal_title：Microbes and environments

authors： Sato Y,Narisawa K,Tsuruta K,Umezu M,Nishizawa T,Tanaka K,Yamaguchi K,Komatsuzaki M,Ohta H

更新日期：2010-01-01 00:00:00

abstract：:The present study describes the occurrence of a unique archaeal ammonia monooxygenase alpha subunit (amoA) gene in nitrifying acid-sulfate soil microcosms at pH 3.5. The soil was collected from an abandoned paddy field in Thailand. Microcosms were incubated in the dark at 30°C for 372 days with the following three tre...

journal_title：Microbes and environments

authors： Nakaya A,Onodera Y,Nakagawa T,Satoh K,Takahashi R,Sasaki S,Tokuyama T

更新日期：2009-01-01 00:00:00

abstract：:Over the past few decades, degradative plasmids have been isolated from bacteria capable of degrading a variety of both natural and man-made compounds. Degradative plasmids belonging to three incompatibility (Inc) groups in Pseudomonas (IncP-1, P-7, and P-9) have been well studied in terms of their replication, mainte...

journal_title：Microbes and environments

authors： Shintani M,Takahashi Y,Yamane H,Nojiri H

更新日期：2010-01-01 00:00:00

abstract：:The role of microbes in the early development of ecosystems on new volcanic materials seems to be crucial to primary plant succession but is not well characterized. Here we analyzed the bacterial community colonizing 22-year-old volcanic deposits of the Miyake-jima Island (Japan) using culture-based and 16S rRNA gene ...

journal_title：Microbes and environments

authors： Lu H,Fujimura R,Sato Y,Nanba K,Kamijo T,Ohta H

更新日期：2008-01-01 00:00:00

abstract：:Fusarium oxysporum is an ascomycetous fungus that is well-known as a soilborne plant pathogen. In addition, a large population of nonpathogenic F. oxysporum (NPF) inhabits various environmental niches, including the phytosphere. To obtain an insight into the origin of plant pathogenic F. oxysporum, we focused on the t...

journal_title：Microbes and environments

authors： Inami K,Kashiwa T,Kawabe M,Onokubo-Okabe A,Ishikawa N,Pérez ER,Hozumi T,Caballero LA,de Baldarrago FC,Roco MJ,Madadi KA,Peever TL,Teraoka T,Kodama M,Arie T

更新日期：2014-01-01 00:00:00

abstract：:The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. ...

journal_title：Microbes and environments

authors： Wang Y,Shi T,Huang G,Gong J

更新日期：2018-09-29 00:00:00

abstract：:The diversity and abundance of Burkholderia species in sugarcane field soils were investigated by a 16S rRNA gene-based approach using genus-specific primers. A total of 365,721 sequences generated by the Illumina MiSeq platform were assigned to the genus Burkholderia. Nearly 58% of these sequences were placed in a pr...

journal_title：Microbes and environments

authors： Tago K,Itoh H,Kikuchi Y,Hori T,Sato Y,Nagayama A,Okubo T,Navarro R,Aoyagi T,Hayashi K,Hayatsu M

更新日期：2014-01-01 00:00:00

abstract：:Microbes interact with each other in multicellular communities and this interaction enables certain microorganisms to survive in various environments. Pseudomonas aeruginosa is a highly adaptable bacterium that ubiquitously inhabits diverse environments including soil, marine habitats, plants and animals. Behind this ...

journal_title：Microbes and environments

authors： Tashiro Y,Yawata Y,Toyofuku M,Uchiyama H,Nomura N

更新日期：2013-01-01 00:00:00