Abstract:
:Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae JCM 10874T. An opdA homolog (opdADSM16289) encoding BNP degradation activity was identified in DSM 16289T, in contrast with JCM 10874T, possessing both the opdA homolog and nmoA. The degradation profile of different BNP isomers was examined by Escherichia coli transformants harboring opdADSM16289, opdAJCM10874, and nmoAJCM10874 to characterize and compare the expression activities of these genes.
journal_name
Microbes Environjournal_title
Microbes and environmentsauthors
Ootsuka M,Nishizawa T,Hasegawa M,Kurusu Y,Ohta Hdoi
10.1264/jsme2.ME18077subject
Has Abstractpub_date
2018-12-28 00:00:00pages
450-454issue
4eissn
1342-6311issn
1347-4405journal_volume
33pub_type
杂志文章abstract::Chemosynthetic microbial communities develop and form dense cell aggregates in slightly alkaline sulfidic hot springs in the temperature range of 70-86°C at Nakabusa, Japan. Nitrogenase activity has recently been detected in the microbial communities collected. To identify possible members capable of nitrogen fixation...
journal_title:Microbes and environments
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