Comparative Analysis of the Genetic Basis of Branched Nonylphenol Degradation by Sphingobium amiense DSM 16289T and Sphingobium cloacae JCM 10874T.

Abstract:

:Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae JCM 10874T. An opdA homolog (opdADSM16289) encoding BNP degradation activity was identified in DSM 16289T, in contrast with JCM 10874T, possessing both the opdA homolog and nmoA. The degradation profile of different BNP isomers was examined by Escherichia coli transformants harboring opdADSM16289, opdAJCM10874, and nmoAJCM10874 to characterize and compare the expression activities of these genes.

journal_name

Microbes Environ

authors

Ootsuka M,Nishizawa T,Hasegawa M,Kurusu Y,Ohta H

doi

10.1264/jsme2.ME18077

subject

Has Abstract

pub_date

2018-12-28 00:00:00

pages

450-454

issue

4

eissn

1342-6311

issn

1347-4405

journal_volume

33

pub_type

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