Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue.

Abstract:

:Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Kit (Qiagen) and the PowerSoil DNA Isolation Kit (Mo Bio Laboratories), followed by next-generation (Illumina) sequencing of partial 16S rRNA genes. Significant differences were detected in microbial community diversity and structure between ascidian species, but not between kits, suggesting similar recovery of biological variation and low technical variation between the two extraction methods for ascidian microbiome characterization.

journal_name

Microbes Environ

authors

Evans JS,López-Legentil S,Erwin PM

doi

10.1264/jsme2.ME18031

subject

Has Abstract

pub_date

2018-12-28 00:00:00

pages

435-439

issue

4

eissn

1342-6311

issn

1347-4405

journal_volume

33

pub_type

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