Isolation and characterization of aerobic thermophilic bacteria from the savusavu hot springs in fiji.

abstract：:The genome of Streptomyces scabies, the predominant causal agent of potato common scab, encodes a potential cutinase, the protein Sub1, which was previously shown to be specifically induced in the presence of suberin. The sub1 gene was expressed in Escherichia coli and the recombinant protein Sub1 was purified and cha...

journal_title：Microbes and environments

authors： Jabloune R,Khalil M,Ben Moussa IE,Simao-Beaunoir AM,Lerat S,Brzezinski R,Beaulieu C

更新日期：2020-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a group of small non-coding RNAs that suppress the expression of target mRNAs. The seed sequence of miRNA plays a crucial role in recognizing the 3'-untranslated region of the target mRNA. Cells infected with a simian foamy virus (SFV) isolated from an African green monkey (Chlorocebus aethiops)...

journal_title：Microbes and environments

authors： Hashimoto-Gotoh A,Kitao K,Miyazawa T

更新日期：2020-01-01 00:00:00

abstract：:To clarify altitudinal changes in the bacterial community on Gulkana Glacier in Alaska, we analyzed bacterial 16S rRNA gene by low-cycle PCR amplification, denaturing gradient gel electrophoresis (DGGE), and culturing in a snowmelt medium at 4°C. Low-cycle PCR-based cloning revealed the presence of 100 bacterial OTUs;...

journal_title：Microbes and environments

authors： Segawa T,Takeuchi N,Ushida K,Kanda H,Kohshima S

更新日期：2010-01-01 00:00:00

abstract：:Termites depend nutritionally on their gut microbes, and protistan, bacterial, and archaeal gut communities have been extensively studied. However, limited information is available on viruses in the termite gut. We herein report the complete genome sequence (99,517 bp) of a phage obtained during a genome analysis of "...

journal_title：Microbes and environments

authors： Pramono AK,Kuwahara H,Itoh T,Toyoda A,Yamada A,Hongoh Y

更新日期：2017-06-24 00:00:00

abstract：:The nifV gene encodes homocitrate synthase, the enzyme that catalyzes the formation of homocitrate, which is essential for arranging the FeMo-cofactor in the catalytic center of nitrogenase. Some host plants, such as Lotus japonicus, supply homocitrate to their symbionts, in this case, Mesorhizobium loti, which lacks ...

journal_title：Microbes and environments

authors： Hashimoto S,Wongdee J,Songwattana P,Greetatorn T,Goto K,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2019-12-27 00:00:00

abstract：:The role of microbes in the early development of ecosystems on new volcanic materials seems to be crucial to primary plant succession but is not well characterized. Here we analyzed the bacterial community colonizing 22-year-old volcanic deposits of the Miyake-jima Island (Japan) using culture-based and 16S rRNA gene ...

journal_title：Microbes and environments

authors： Lu H,Fujimura R,Sato Y,Nanba K,Kamijo T,Ohta H

更新日期：2008-01-01 00:00:00

abstract：:We established artificial consortia of bacteria inoculated from soil and a green alga, Chlorella vulgaris NIES-227. The bacteria and the alga were mixed in a tube or partitioned in a dialysing culture vessel, and the bacterial composition was examined after cultivation. The community of bacteria formed in the consorti...

journal_title：Microbes and environments

authors： Ueda H,Otsuka S,Senoo K

更新日期：2010-01-01 00:00:00

abstract：:Ultra-small microorganisms are ubiquitous in Earth's environments. Ultramicrobacteria, which are defined as having a cell volume of <0.1 μm3, are often numerically dominant in aqueous environments. Cultivated representatives among these bacteria, such as members of the marine SAR11 clade (e.g., "Candidatus Pelagibacte...

journal_title：Microbes and environments

authors： Nakai R

更新日期：2020-01-01 00:00:00

abstract：:Fusarium oxysporum is an ascomycetous fungus that is well-known as a soilborne plant pathogen. In addition, a large population of nonpathogenic F. oxysporum (NPF) inhabits various environmental niches, including the phytosphere. To obtain an insight into the origin of plant pathogenic F. oxysporum, we focused on the t...

journal_title：Microbes and environments

authors： Inami K,Kashiwa T,Kawabe M,Onokubo-Okabe A,Ishikawa N,Pérez ER,Hozumi T,Caballero LA,de Baldarrago FC,Roco MJ,Madadi KA,Peever TL,Teraoka T,Kodama M,Arie T

更新日期：2014-01-01 00:00:00

abstract：:The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxi...

journal_title：Microbes and environments

authors： Yanagawa K,Tani A,Yamamoto N,Hachikubo A,Kano A,Matsumoto R,Suzuki Y

更新日期：2016-06-25 00:00:00

abstract：:The screening of pollutant degraders by relying solely on cultivation techniques such as liquid enrichment often fails to isolate the actual degraders in the environment. Community analyses by PCR-denaturing gradient gel electrophoresis (DGGE) were performed to isolate bacteria that can degrade 3-chlorobenzoate (3CB) ...

journal_title：Microbes and environments

authors： Morimoto S,Ogawa N,Hasebe A,Fujii T

更新日期：2008-01-01 00:00:00

abstract：:Soil and rhizosphere environments were examined in order to determine the identity and relative abundance of bacteria that catabolize d- and l-amino acids as the sole source of carbon and nitrogen. All substrates were readily catabolized by bacteria from both environments, with most d-amino acids giving similar CFU co...

journal_title：Microbes and environments

authors： Radkov AD,McNeill K,Uda K,Moe LA

更新日期：2016-06-25 00:00:00

abstract：:Arsenic (As) is a widely distributed toxic element in the environment and microorganisms have developed resistance mechanisms in order to tolerate it. The cellular response of the chemoorganotrophic arsenite (As[III])-oxidizing α-Proteobacteria, Sinorhizobium sp. strain A2, to arsenic was examined in the present study...

journal_title：Microbes and environments

authors： Fukushima K,Huang H,Hamamura N

更新日期：2015-01-01 00:00:00

abstract：:A cellular approach combining Direct Viable Counting and Fluorescent In Situ Hybridization using a one-step multiple-probe technique and Solid Phase Cytometry (DVC-FISH-SPC) was developed to monitor total viable vibrios and cover the detection of a large diversity of vibrios. FISH combined three probes in the same ass...

journal_title：Microbes and environments

authors： Girard L,Peuchet S,Servais P,Henry A,Charni-Ben-Tabassi N,Baudart J

更新日期：2017-09-27 00:00:00

abstract：:Members of the Marseilleviridae family are large DNA viruses with icosahedral particle structures that infect Acanthamoeba cells. The first Marseillevirus to be discovered was isolated in 2009. Since then, several other members of the Marseilleviridae family have been reported, including Lausannevirus, Senegalvirus, C...

journal_title：Microbes and environments

authors： Takemura M

更新日期：2016-12-23 00:00:00

abstract：:In the present study, 77 strains of endophytic actinomycetes isolated from cabbage were screened in order to assess their biocontrol potential against Alternaria brassicicola on cabbage seedlings. In the first and second screening trials, cabbage seedlings pretreated with mycelial suspensions of each isolate were spra...

journal_title：Microbes and environments

authors： Hassan N,Nakasuji S,Elsharkawy MM,Naznin HA,Kubota M,Ketta H,Shimizu M

更新日期：2017-06-24 00:00:00

abstract：:Over the past few decades, degradative plasmids have been isolated from bacteria capable of degrading a variety of both natural and man-made compounds. Degradative plasmids belonging to three incompatibility (Inc) groups in Pseudomonas (IncP-1, P-7, and P-9) have been well studied in terms of their replication, mainte...

journal_title：Microbes and environments

authors： Shintani M,Takahashi Y,Yamane H,Nojiri H

更新日期：2010-01-01 00:00:00

abstract：:Oligosaline lakes in arid regions provide indispensable water resources for humans; however, information on the bacterial community composition (BCC) of this ecosystem is limited. In the present study, we explored seasonal and vertical variations in BCC in Lake Bosten, a unique oligosaline lake (1.2‰ salinity) in arid...

journal_title：Microbes and environments

authors： Tang X,Xie G,Shao K,Dai J,Chen Y,Xu Q,Gao G

更新日期：2015-01-01 00:00:00

abstract：:Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still con...

journal_title：Microbes and environments

authors： Urashima Y,Sonoda T,Fujita Y,Uragami A

更新日期：2012-01-01 00:00:00

abstract：:A Gram-positive marine bacterium, Exiguobacterium sp. SBH81, was isolated as a hydrophilic organic-solvent tolerant bacterium, and exhibited high tolerance to various types of toxic hydrophilic organic solvents, including acetonitrile, at relatively high concentrations (up to 6% [v/v]) under the growing conditions. In...

journal_title：Microbes and environments

authors： Kongpol A,Kato J,Tajima T,Vangnai AS

更新日期：2012-01-01 00:00:00

abstract：:Denitrifiers can produce and consume nitrous oxide (N(2)O). While little N(2)O is emitted from rice paddy soil, the same soil produces N(2)O when the land is drained and used for upland crop cultivation. In this study, we collected soils from two types of fields each at three locations in Japan; one type of field had ...

journal_title：Microbes and environments

authors： Tago K,Ishii S,Nishizawa T,Otsuka S,Senoo K

更新日期：2011-01-01 00:00:00

abstract：:Members of the Legionella genus find suitable conditions for their growth and survival in nuclear power plant cooling circuits. To limit the proliferation of Legionella pathogenic bacteria in nuclear power plant cooling circuits, and ensure that levels remain below regulatory thresholds, monochloramine treatment can b...

journal_title：Microbes and environments

authors： Jakubek D,Guillaume C,Binet M,Leblon G,DuBow M,Le Brun M

更新日期：2013-01-01 00:00:00

abstract：:Due to their obligate symbiotic nature and lack of long-term storage methods, the strain collection of arbuscular mycorrhizal (AM) fungi requires periodic proliferation using a pot culture with host plants. Therefore, a method to evaluate the purity of proliferated AM fungal cultures is critical for the quality contro...

journal_title：Microbes and environments

authors： Ohtomo R,Oka N,Morimoto S

更新日期：2019-12-27 00:00:00

abstract：:Chemosynthetic microbial communities develop and form dense cell aggregates in slightly alkaline sulfidic hot springs in the temperature range of 70-86°C at Nakabusa, Japan. Nitrogenase activity has recently been detected in the microbial communities collected. To identify possible members capable of nitrogen fixation...

journal_title：Microbes and environments

authors： Nishihara A,Thiel V,Matsuura K,McGlynn SE,Haruta S

更新日期：2018-12-28 00:00:00

abstract：:Potato peels consist of a tissue called phellem, which is formed by suberized cell layers. The degradation of suberin, a lipidic and recalcitrant polymer, is an ecological process attributed to soil fungal populations; however, previous studies have suggested that Streptomyces scabiei, the causal agent of potato commo...

journal_title：Microbes and environments

authors： Beaulieu C,Sidibé A,Jabloune R,Simao-Beaunoir AM,Lerat S,Monga E,Bernards MA

更新日期：2016-12-23 00:00:00

abstract：:This study aimed to characterize soil fungal communities in upland rice fields managed with tillage/non-tillage and winter cover-cropping (hairy vetch and cereal rye) practices, using PCR-based molecular methods. The study plots were maintained as upland fields for 5 years and the soils sampled in the second and fifth...

journal_title：Microbes and environments

authors： Nishizawa T,Zhaorigetu,Komatsuzaki M,Sato Y,Kaneko N,Ohta H

更新日期：2010-01-01 00:00:00

abstract：:Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite red...

journal_title：Microbes and environments

authors： Kobayashi S,Hira D,Yoshida K,Toyofuku M,Shida Y,Ogasawara W,Yamaguchi T,Araki N,Oshiki M

更新日期：2018-12-28 00:00:00

abstract：:The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotu...

journal_title：Microbes and environments

authors： Hashimoto S,Goto K,Pyromyou P,Songwattana P,Greetatorn T,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

更新日期：2020-01-01 00:00:00

abstract：:Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Ki...

journal_title：Microbes and environments

authors： Evans JS,López-Legentil S,Erwin PM

更新日期：2018-12-28 00:00:00

abstract：:HcRNAV is a small icosahedral virus that infects the shellfish-killing marine dinoflagellate Heterocapsa circularisquama, which harbors a dicistronic linear single-stranded RNA (ssRNA) genome ca. 4.4 kb in length. Its major capsid protein (MCP) gene sequence is not expressed by various strains of Escherichia coli, pos...

journal_title：Microbes and environments

authors： Wada K,Kimura K,Hasegawa A,Fukuyama K,Nagasaki K

更新日期：2012-01-01 00:00:00