Discovery and Complete Genome Sequence of a Bacteriophage from an Obligate Intracellular Symbiont of a Cellulolytic Protist in the Termite Gut.

Abstract:

:Termites depend nutritionally on their gut microbes, and protistan, bacterial, and archaeal gut communities have been extensively studied. However, limited information is available on viruses in the termite gut. We herein report the complete genome sequence (99,517 bp) of a phage obtained during a genome analysis of "Candidatus Azobacteroides pseudotrichonymphae" phylotype ProJPt-1, which is an obligate intracellular symbiont of the cellulolytic protist Pseudotrichonympha sp. in the gut of the termite Prorhinotermes japonicus. The genome of the phage, designated ProJPt-Bp1, was circular or circularly permuted, and was not integrated into the two circular chromosomes or five circular plasmids composing the host ProJPt-1 genome. The phage was putatively affiliated with the order Caudovirales based on sequence similarities with several phage-related genes; however, most of the 52 protein-coding sequences had no significant homology to sequences in the databases. The phage genome contained a tRNA-Gln (CAG) gene, which showed the highest sequence similarity to the tRNA-Gln (CAA) gene of the host "Ca. A. pseudotrichonymphae" phylotype ProJPt-1. Since the host genome lacked a tRNA-Gln (CAG) gene, the phage tRNA gene may compensate for differences in codon usage bias between the phage and host genomes. The phage genome also contained a non-coding region with high nucleotide sequence similarity to a region in one of the host plasmids. No other phage-related sequences were found in the host ProJPt-1 genome. To the best of our knowledge, this is the first report of a phage from an obligate, mutualistic endosymbiont permanently associated with eukaryotic cells.

journal_name

Microbes Environ

authors

Pramono AK,Kuwahara H,Itoh T,Toyoda A,Yamada A,Hongoh Y

doi

10.1264/jsme2.ME16175

subject

Has Abstract

pub_date

2017-06-24 00:00:00

pages

112-117

issue

2

eissn

1342-6311

issn

1347-4405

journal_volume

32

pub_type

杂志文章
  • Microsatellite-primed PCR for intra-species genetic relatedness in Trichophyton ajelloi strains isolated in poland from various soil samples.

    abstract::Trichophyton ajelloi is a geophilic dermatophyte that specializes in the decomposition of native keratin. It exists in soil with a permanent influx of keratin matter. In the present study, two PCR-based methods were used for the identification and intra-species differentiation of T. ajelloi strains isolated from 3 typ...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me13160

    authors: Ciesielska A,Bohacz J,Korniłłowicz-Kowalska T,Stączek P

    更新日期:2014-01-01 00:00:00

  • Levels of Periplasmic Nitrate Reductase during Denitrification are Lower in Bradyrhizobium japonicum than in Bradyrhizobium diazoefficiens.

    abstract::Soybean plants host endosymbiotic dinitrogen (N2)-fixing bacteria from the genus Bradyrhizobium. Under oxygen-limiting conditions, Bradyrhizobium diazoefficiens and Bradyrhizobium japonicum perform denitrification by sequentially reducing nitrate (NO3-) to nitrous oxide (N2O) or N2. The anaerobic reduction of NO3- to ...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME19129

    authors: Siqueira AF,Sugawara M,Arashida H,Minamisawa K,Sánchez C

    更新日期:2020-01-01 00:00:00

  • Comparative analysis of bacterial and archaeal communities in methanogenic sludge granules from upflow anaerobic sludge blanket reactors treating various food-processing, high-strength organic wastewaters.

    abstract::A comprehensive survey of bacterial and archaeal community structures within granular sludges taken from twelve different types of full-scale, food-processing wastewater-treating, upflow anaerobic sludge blanket (UASB) reactors was performed with a 16S rRNA gene-based clone library method. In total, 1,282 bacterial 16...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me08561

    authors: Narihiro T,Terada T,Kikuchi K,Iguchi A,Ikeda M,Yamauchi T,Shiraishi K,Kamagata Y,Nakamura K,Sekiguchi Y

    更新日期:2009-01-01 00:00:00

  • Molecular Detection of Eukaryotic Diets and Gut Mycobiomes in Two Marine Sediment-Dwelling Worms, Sipunculus nudus and Urechis unicinctus.

    abstract::The present study aimed to reveal the eukaryotic diets of two economically important marine sediment-inhabiting worms, Sipunculus nudus (peanut worm) and Urechis unicinctus (spoon worm), using clone libraries and phylogenetic analyses of 18S rRNA genes. Fungal rDNA was also targeted and analyzed to reveal mycobiomes. ...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME18065

    authors: Wang Y,Shi T,Huang G,Gong J

    更新日期:2018-09-29 00:00:00

  • Characterization of herbaspirillum- and limnobacter-related strains isolated from young volcanic deposits in miyake-jima island, Japan.

    abstract::The role of microbes in the early development of ecosystems on new volcanic materials seems to be crucial to primary plant succession but is not well characterized. Here we analyzed the bacterial community colonizing 22-year-old volcanic deposits of the Miyake-jima Island (Japan) using culture-based and 16S rRNA gene ...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.23.66

    authors: Lu H,Fujimura R,Sato Y,Nanba K,Kamijo T,Ohta H

    更新日期:2008-01-01 00:00:00

  • Detection of betaproteobacteria inside the mycelium of the fungus Mortierella elongata.

    abstract::Microscopic and molecular analyses showed the presence of endobacteria inside the mycelia of four out of twelve nitrous oxide (N(2)O)-producing fungal isolates identified as Mortierella elongata. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal strains a...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me10134

    authors: Sato Y,Narisawa K,Tsuruta K,Umezu M,Nishizawa T,Tanaka K,Yamaguchi K,Komatsuzaki M,Ohta H

    更新日期:2010-01-01 00:00:00

  • Comparative Analysis of the Genetic Basis of Branched Nonylphenol Degradation by Sphingobium amiense DSM 16289T and Sphingobium cloacae JCM 10874T.

    abstract::Branched nonylphenol (BNP), a degradation product of nonylphenol polyethoxylates, exerts estrogenic effects on various organisms. The genes underlying BNP degradation by Sphingobium amiense DSM 16289T were analyzed by complete genome sequencing and compared with those of the versatile BNP-degrading Sphingobium cloacae...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME18077

    authors: Ootsuka M,Nishizawa T,Hasegawa M,Kurusu Y,Ohta H

    更新日期:2018-12-28 00:00:00

  • Establishment of a bacterial expression system and immunoassay platform for the major capsid protein of HcRNAV, a dinoflagellate-infecting RNA virus.

    abstract::HcRNAV is a small icosahedral virus that infects the shellfish-killing marine dinoflagellate Heterocapsa circularisquama, which harbors a dicistronic linear single-stranded RNA (ssRNA) genome ca. 4.4 kb in length. Its major capsid protein (MCP) gene sequence is not expressed by various strains of Escherichia coli, pos...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me12046

    authors: Wada K,Kimura K,Hasegawa A,Fukuyama K,Nagasaki K

    更新日期:2012-01-01 00:00:00

  • Persistent Infection of Simian Foamy Virus Derived from the Japanese Macaque Leads to the High-Level Expression of microRNA that Resembles the miR-1 microRNA Precursor Family.

    abstract::MicroRNAs (miRNAs) are a group of small non-coding RNAs that suppress the expression of target mRNAs. The seed sequence of miRNA plays a crucial role in recognizing the 3'-untranslated region of the target mRNA. Cells infected with a simian foamy virus (SFV) isolated from an African green monkey (Chlorocebus aethiops)...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME19130

    authors: Hashimoto-Gotoh A,Kitao K,Miyazawa T

    更新日期:2020-01-01 00:00:00

  • Distribution of N-Acylhomoserine Lactone-Producing Fluorescent Pseudomonads in the Phyllosphere and Rhizosphere of Potato (Solanum tuberosum L.).

    abstract::Four hundred and fifty nine isolates of fluorescent pseudomonads were obtained from the leaves and roots of potato plants. Of these, 20 leaf isolates and 28 root isolates induced violacein production in two N-acylhomoserine lactone (AHL)-reporter strains-Chromobacterium violaceum CV026 and VIR24. VIR24 is a new report...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me09155

    authors: Someya N,Morohoshi T,Okano N,Otsu E,Usuki K,Sayama M,Sekiguchi H,Ikeda T,Ishida S

    更新日期:2009-01-01 00:00:00

  • Susceptibility of Legionella strains to the chlorinated biocide, monochloramine.

    abstract::Members of the Legionella genus find suitable conditions for their growth and survival in nuclear power plant cooling circuits. To limit the proliferation of Legionella pathogenic bacteria in nuclear power plant cooling circuits, and ensure that levels remain below regulatory thresholds, monochloramine treatment can b...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me12205

    authors: Jakubek D,Guillaume C,Binet M,Leblon G,DuBow M,Le Brun M

    更新日期:2013-01-01 00:00:00

  • Inactivation of bacteria in freshwater by momentary decompression following high pressurization.

    abstract::Rapid and continuous pressure treatment was realized using a hydraulic pump and the momentary decompression following high pressurization was used to inactivate bacteria. The number of colony-forming E. coli decreased to 1/1000 in response to 10 cycles of pressure treatment. In groundwater samples, repeated pressure t...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me10175

    authors: Baba T,Yamaguchi N,Tani K,Nasu M

    更新日期:2011-01-01 00:00:00

  • Inhibitory effects of ferrihydrite on a thermophilic methanogenic community.

    abstract::The addition of ferrihydrite to methanogenic microbial communities obtained from a thermophilic anaerobic digester suppressed methanogenesis in a dose-dependent manner. The amount of reducing equivalents consumed by the reduction of iron was significantly smaller than that expected from the decrease in the production ...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me14026

    authors: Yamada C,Kato S,Ueno Y,Ishii M,Igarashi Y

    更新日期:2014-01-01 00:00:00

  • Isolation of fucosyltransferase-producing bacteria from marine environments.

    abstract::Fucose-containing oligosaccharides on the cell surface of some pathogenic bacteria are thought to be important for host-microbe interactions and to play a major role in the pathogenicity of bacterial pathogens. Here, we screened marine bacteria for glycosyltransferases using two methods: a one-pot glycosyltransferase ...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me12058

    authors: Kajiwara H,Toda M,Mine T,Nakada H,Yamamoto T

    更新日期:2012-01-01 00:00:00

  • Enzymatic Degradation of p-Nitrophenyl Esters, Polyethylene Terephthalate, Cutin, and Suberin by Sub1, a Suberinase Encoded by the Plant Pathogen Streptomyces scabies.

    abstract::The genome of Streptomyces scabies, the predominant causal agent of potato common scab, encodes a potential cutinase, the protein Sub1, which was previously shown to be specifically induced in the presence of suberin. The sub1 gene was expressed in Escherichia coli and the recombinant protein Sub1 was purified and cha...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME19086

    authors: Jabloune R,Khalil M,Ben Moussa IE,Simao-Beaunoir AM,Lerat S,Brzezinski R,Beaulieu C

    更新日期:2020-01-01 00:00:00

  • Temperature-dependent variations in sulfate-reducing communities associated with a terrestrial hydrocarbon seep.

    abstract::Terrestrial hydrocarbon seeps are an important source of naturally emitted methane over geological time. The exact community compositions responsible for carbon cycling beneath these surface features remain obscure. As sulfate reduction represents an essential process for anoxic organic mineralization, this study coll...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME14086

    authors: Cheng TW,Lin LH,Lin YT,Song SR,Wang PL

    更新日期:2014-01-01 00:00:00

  • Diversity of Methane-Oxidizing Bacteria in Soils from "Hot Lands of Medolla" (Italy) Featured by Anomalous High-Temperatures and Biogenic CO2 Emission.

    abstract::"Terre Calde di Medolla" (TCM) (literally, "Hot Lands of Medolla") refers to a farming area in Italy with anomalously high temperatures and diffuse emissions of biogenic CO2, which has been linked to CH4 oxidation processes from a depth of 0.7 m to the surface. We herein assessed the composition of the total bacterial...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME16087

    authors: Cappelletti M,Ghezzi D,Zannoni D,Capaccioni B,Fedi S

    更新日期:2016-12-23 00:00:00

  • Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment.

    abstract::The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanis...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me11338

    authors: Nonaka L,Maruyama F,Miyamoto M,Miyakoshi M,Kurokawa K,Masuda M

    更新日期:2012-01-01 00:00:00

  • Morphological and Taxonomic Properties of Tokyovirus, the First Marseilleviridae Member Isolated from Japan.

    abstract::Members of the Marseilleviridae family are large DNA viruses with icosahedral particle structures that infect Acanthamoeba cells. The first Marseillevirus to be discovered was isolated in 2009. Since then, several other members of the Marseilleviridae family have been reported, including Lausannevirus, Senegalvirus, C...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME16107

    authors: Takemura M

    更新日期:2016-12-23 00:00:00

  • Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis.

    abstract::Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite red...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME18058

    authors: Kobayashi S,Hira D,Yoshida K,Toyofuku M,Shida Y,Ogasawara W,Yamaguchi T,Araki N,Oshiki M

    更新日期:2018-12-28 00:00:00

  • Altitudinal changes in a bacterial community on Gulkana Glacier in Alaska.

    abstract::To clarify altitudinal changes in the bacterial community on Gulkana Glacier in Alaska, we analyzed bacterial 16S rRNA gene by low-cycle PCR amplification, denaturing gradient gel electrophoresis (DGGE), and culturing in a snowmelt medium at 4°C. Low-cycle PCR-based cloning revealed the presence of 100 bacterial OTUs;...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me10119

    authors: Segawa T,Takeuchi N,Ushida K,Kanda H,Kohshima S

    更新日期:2010-01-01 00:00:00

  • Comparing Two Common DNA Extraction Kits for the Characterization of Symbiotic Microbial Communities from Ascidian Tissue.

    abstract::Various DNA extraction methods are often used interchangeably for the characterization of microbial communities despite indications that different techniques produce disparate results. The microbiomes of two ascidian species were herein characterized using two common DNA extraction kits, the DNeasy Blood and Tissue Ki...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME18031

    authors: Evans JS,López-Legentil S,Erwin PM

    更新日期:2018-12-28 00:00:00

  • Size Matters: Ultra-small and Filterable Microorganisms in the Environment.

    abstract::Ultra-small microorganisms are ubiquitous in Earth's environments. Ultramicrobacteria, which are defined as having a cell volume of <0.1 μm3, are often numerically dominant in aqueous environments. Cultivated representatives among these bacteria, such as members of the marine SAR11 clade (e.g., "Candidatus Pelagibacte...

    journal_title:Microbes and environments

    pub_type: 杂志文章,评审

    doi:10.1264/jsme2.ME20025

    authors: Nakai R

    更新日期:2020-01-01 00:00:00

  • Type III Secretion System of Bradyrhizobium sp. SUTN9-2 Obstructs Symbiosis with Lotus spp.

    abstract::The rhizobial type III secretion system secretes effector proteins into host plant cells, which may either promote or inhibit symbiosis with legumes. We herein demonstrated that the type III secretion system of Bradyrhizobium sp. SUTN9-2 obstructed symbiosis with Lotus japonicus Miyakojima, L. japonicus Gifu, and Lotu...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME20041

    authors: Hashimoto S,Goto K,Pyromyou P,Songwattana P,Greetatorn T,Tittabutr P,Boonkerd N,Teaumroong N,Uchiumi T

    更新日期:2020-01-01 00:00:00

  • Biogeochemical Cycle of Methanol in Anoxic Deep-Sea Sediments.

    abstract::The biological flux and lifetime of methanol in anoxic marine sediments are largely unknown. We herein reported, for the first time, quantitative methanol removal rates in subsurface sediments. Anaerobic incubation experiments with radiotracers showed high rates of microbial methanol consumption. Notably, methanol oxi...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME15204

    authors: Yanagawa K,Tani A,Yamamoto N,Hachikubo A,Kano A,Matsumoto R,Suzuki Y

    更新日期:2016-06-25 00:00:00

  • Analysis of ammonia monooxygenase and archaeal 16S rRNA gene fragments in nitrifying acid-sulfate soil microcosms.

    abstract::The present study describes the occurrence of a unique archaeal ammonia monooxygenase alpha subunit (amoA) gene in nitrifying acid-sulfate soil microcosms at pH 3.5. The soil was collected from an abandoned paddy field in Thailand. Microcosms were incubated in the dark at 30°C for 372 days with the following three tre...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me09104

    authors: Nakaya A,Onodera Y,Nakagawa T,Satoh K,Takahashi R,Sasaki S,Tokuyama T

    更新日期:2009-01-01 00:00:00

  • Distribution and evolution of nitrogen fixation genes in the phylum Bacteroidetes.

    abstract::Diazotrophs had not previously been identified among bacterial species in the phylum Bacteroidetes until the rapid expansion of bacterial genome sequences, which revealed the presence of nitrogen fixation (nif) genes in this phylum. We herein determined the draft genome sequences of Bacteroides graminisolvens JCM 1509...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.ME14142

    authors: Inoue J,Oshima K,Suda W,Sakamoto M,Iino T,Noda S,Hongoh Y,Hattori M,Ohkuma M

    更新日期:2015-01-01 00:00:00

  • Application of PCR-denaturing-gradient gel electrophoresis (DGGE) method to examine microbial community structure in asparagus fields with growth inhibition due to continuous cropping.

    abstract::Growth inhibition due to continuous cropping of asparagus is a major problem; the yield of asparagus in replanted fields is low compared to that in new fields, and missing plants occur among young seedlings. Although soil-borne disease and allelochemicals are considered to be involved in this effect, this is still con...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me11222

    authors: Urashima Y,Sonoda T,Fujita Y,Uragami A

    更新日期:2012-01-01 00:00:00

  • Interspecies interaction between Pseudomonas aeruginosa and other microorganisms.

    abstract::Microbes interact with each other in multicellular communities and this interaction enables certain microorganisms to survive in various environments. Pseudomonas aeruginosa is a highly adaptable bacterium that ubiquitously inhabits diverse environments including soil, marine habitats, plants and animals. Behind this ...

    journal_title:Microbes and environments

    pub_type: 杂志文章,评审

    doi:10.1264/jsme2.me12167

    authors: Tashiro Y,Yawata Y,Toyofuku M,Uchiyama H,Nomura N

    更新日期:2013-01-01 00:00:00

  • Degradation of N-acylhomoserine lactone quorum sensing signaling molecules by potato root surface-associated Chryseobacterium strains.

    abstract::N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signaling molecules by many Gram-negative bacteria. Here, 413 bacterial strains were obtained from the roots of potato plants and screened for AHL-degrading bacteria using Chromobacterium violaceum reporter strains. Sixty one isolates degraded N-hexanoyl-L-ho...

    journal_title:Microbes and environments

    pub_type: 杂志文章

    doi:10.1264/jsme2.me10207

    authors: Rashid R,Morohoshi T,Someya N,Ikeda T

    更新日期:2011-01-01 00:00:00