Abstract:
:The splicing of a bacterial group II subclass B intron B.me.I1 from Bacillus megaterium chromosomes was investigated. RT-PCR and nucleic acid hybridization methods were used to understand the role of the intron-encoded protein (IEP) in the splicing of B.me.I1. An in vivo assay showed that the splicing occurred in the absence of IEP. An in vitro assay showed that B.me.I1 was spliced under conditions similar to those of the intracellular environment with no help from other biological molecules. Because all group II introns previously reported needed IEPs for their splicing in vivo, our results suggest that B.me.I1 is an "actual" self-splicing group II intron. This is also the first report to recognize the existence of group II introns that independently splice mRNA in vivo. The self-splicing of a bacterial intron may support that eukaryotic spliceosomal introns originated in bacterial genomes.
journal_name
Microbes Environjournal_title
Microbes and environmentsauthors
Chien MF,Tosa S,Huang CC,Endo Gdoi
10.1264/jsme2.me08540subject
Has Abstractpub_date
2009-01-01 00:00:00pages
28-32issue
1eissn
1342-6311issn
1347-4405pii
JST.JSTAGE/jsme2/ME08540journal_volume
24pub_type
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journal_title:Microbes and environments
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更新日期:2019-09-25 00:00:00
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journal_title:Microbes and environments
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更新日期:2011-01-01 00:00:00
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