Abstract:
BACKGROUND:The transcription factor SOX2 controls gene expression during development and has roles in both neurogenesis and gliogenesis. MATERIALS AND METHODS:The current study utilized immunohistochemistry, immunofluorescence, molecular genetics (gene copy number analysis) and Western blotting to examine SOX2 expression in surgical samples from 133 brain gliomas of different grades of malignancy, and in cell lines from 16 glioblastomas. RESULTS:Our results reveal a positive correlation between SOX2 expression and malignancy grade in gliomas and identify the hypercellular and hyperproliferative areas of glioblastomas as the areas with the highest SOX2 expression. SOX2 gene amplification was found in neurospheres and 14.4% and 11.1% of glioblastomas and anaplastic oligodendrogliomas, respectively. In contrast, SOX2 expression was not observed in neuronal tumors and varied in medulloblastomas depending upon their neuronal differentiation status. CONCLUSION:It is concluded that SOX2 is a marker for undifferentiated and proliferating cells and that its expression is up-regulated in the most anaplastic areas of glioblastomas and oligodendrogliomas. The hypothesis is that antigenic and genetic analogy exist between these areas and neurospheres and that glioblastoma stem cells may actually be dedifferentiated tumor cells that re-acquire a stem cell-like status.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
Annovazzi L,Mellai M,Caldera V,Valente G,Schiffer Dsubject
Has Abstractpub_date
2011-05-01 00:00:00pages
139-47issue
3eissn
1109-6535issn
1790-6245pii
8/3/139journal_volume
8pub_type
杂志文章abstract::Cellular signal transduction pathways and gene expression are tightly regulated to accommodate changes in response to physiological environments. In the current study, molecules were identified that are activated as a result of intracellular signaling and immediately expressed as mRNA in MCF-7 breast cancer cells shor...
journal_title:Cancer genomics & proteomics
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abstract::Testicular germ cell tumors (GCT) are the most frequent malignancy in young adults and arise from intratubular germ cell neoplasia undetermined (IGCNU, also referred to as carcinoma in situ, CIS). To determine the transcriptional programs involved in the transition from normal germ cells to GCT, and to further elucida...
journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
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journal_title:Cancer genomics & proteomics
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doi:
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2011-07-01 00:00:00
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journal_title:Cancer genomics & proteomics
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更新日期:2012-03-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20098
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20010
更新日期:2016-01-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2006-03-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2015-03-01 00:00:00
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journal_title:Cancer genomics & proteomics
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更新日期:2016-05-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20223
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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journal_title:Cancer genomics & proteomics
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doi:
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journal_title:Cancer genomics & proteomics
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doi:
更新日期:2007-01-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:10.21873/cgp.20069
更新日期:2018-03-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2015-07-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章,评审
doi:
更新日期:2006-05-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Cancer genomics & proteomics
pub_type: 杂志文章
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