Cigarette smoke-induced effects on the cell cycle in oral cancer cells: reduction of G2/M fraction.

Abstract:

BACKGROUND:Cigarette smoke (CS) is the main inducer of oral cancer, increasing its prevalence by 4-7 times. MATERIALS AND METHODS:We examined the suitability of cell models SCC-25 and SCC-15 for studying effects of CS on oral cancer cells and whether CS significantly affects the cell cycle using fluorescence-activated cell sorting assays. RESULTS:There was significant change in the fraction of SCC-15 cells in pre-G1 state following CS exposure. At 60 and 90 min, increase in the pre-G1 cell fraction was 118% (p<0.05) and 135% (p<0.01) respectively. The G2/M cell fraction was significantly lower following CS exposure. At 90 and 120 min following CS exposure, the G2/M fraction levels had declined by 44% (p<0.05) and 34% (p<0.01) respectively. Results for SCC-25 cells were similar. At 90 and 120 min following CS exposure, the pre-G1 fraction of the cells increased by 230% and 550%, respectively (p<0.01). At 120 min of CS exposure, the fraction of G2/M cells was lower by 47% (p<0.05) compared to controls. CONCLUSION:CS profoundly affects the cell-cycle distribution in both SCC-15 and SCC-25 oral cancer cellular models. Such effects have been associated with DNA damage and carcinogenesis. Both models are useful for studying oral cancer pathogenesis.

authors

Krayzler E,Nagler RM

subject

Has Abstract

pub_date

2015-03-01 00:00:00

pages

73-6

issue

2

eissn

1109-6535

issn

1790-6245

pii

12/2/73

journal_volume

12

pub_type

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