Abstract：
:Cellular signal transduction pathways and gene expression are tightly regulated to accommodate changes in response to physiological environments. In the current study, molecules were identified that are activated as a result of intracellular signaling and immediately expressed as mRNA in MCF-7 breast cancer cells shortly after stimulation of ErbB receptor ligands, epidermal growth factor (EGF) or heregulin (HRG). For the identification of tyrosine-phosphorylated proteins and expressed genes, a SILAC (stable isotopic labeling using amino acids in cell culture) method and Affymetrix gene expression array system, respectively, were used. Unexpectedly, the overlapping of genes appeared in two experimental datasets was very low for HRG (43 hits in the proteome data, 1,655 in the transcriptome data, and 5 hits common to both datasets), while no overlapping gene was detected for EGF (15 hits in the proteome data, 211 hits in the transcriptome data, and no hits common to both datasets). The HRG overlapping genes included ERBB2, NEDD9, MAPK3, JUP and EPHA2. Biological pathway analysis indicated that HRG-stimulated molecular activation is significantly related to cancer pathways including bladder cancer, chronic myeloid leukemia and pancreatic cancer (p < 0.05). The proteome datasets of EGF and HRG contain molecules that are related to Axon guidance, ErbB signaling and VEGF signaling at a high rate.
journal_name
Cancer Genomics Proteomicsjournal_title
Cancer genomics & proteomicsauthors
Nagashima T,Oyama M,Kozuka-Hata H,Yumoto N,Sakaki Y,Hatakeyama Msubject
Has Abstractpub_date
2008-05-01 00:00:00pages
161-8issue
3-4eissn
1109-6535issn
1790-6245journal_volume
5pub_type
杂志文章abstract：BACKGROUND/AIM:The aim of this study was to assess the incidence of MSI in a large series of human hepatocellular carcinomas (HCC) with various etiologies. MATERIALS AND METHODS:The MSI status was determined by polymerase chain reaction (PCR) using 5 mononucleotide and 13 CAn dinucleotide repeats. RESULTS:None of the...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20043
更新日期：2017-09-01 00:00:00
abstract：BACKGROUND/AIM:The chromosome translocation t(14;21)(q11;q22) was reported in four pediatric T-cell lymphoblastic leukemias and was shown to activate the OLIG2 gene. MATERIALS AND METHODS:A pediatric T-cell lymphoblastic lymphoma was investigated using G-banding chromosome analysis, fluorescence in situ hybridization ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20166
更新日期：2020-01-01 00:00:00
abstract：BACKGROUND:Cancer is a major public health problem worldwide, and the number of incident cases increases every year expected to reach 17.1 million a year by 2020. There is evidence that people who adhere to the Mediterranean Diet (MediD) have lower incidence of cancer. However, cancers' location and culture studies see...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20050
更新日期：2017-11-01 00:00:00
abstract：:Sepantronium bromide (YM-155) is believed to elicit apoptosis and mitotic arrest in tumor cells by reducing (BIRC5, survivin) mRNA. In this study, we monitored changes in survivin mRNA and protein after treating MDA-MB-231 cells with YM-155 concurrent with evaluation of whole transcriptomic (WT) mRNA and long intergen...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20083
更新日期：2018-07-01 00:00:00
abstract：AIM:Alpha B-Crystallin (CRYAB) is purported to be a metastasis suppressor protein, and lack or lower CRYAB expression is a prognostic biomarker for several types of cancer, such as that of the prostate and head and neck. However, the association of genomic variation of CRYAB and breast cancer is not well studied. The a...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-09-01 00:00:00
abstract：:Although the statistical probability of therapeutic success is known for larger groups of cancer patients, the clinical response to chemotherapy of the individual patient remains uncertain. It would be of great value to know whether or not an individual tumor responds to the proposed therapy. The concept of sensitivit...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2004-03-01 00:00:00
abstract：BACKGROUND/AIM:The outcome of patients with advanced hepatocellular carcinoma (HCC) remains poor and therapeutic options, including sorafenib, the first anti-cancer drug proved to prolong survival in patients with advanced HCC, are limited. However, no clinically useful predictive biomarker for sorafenib has been repor...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20159
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:Oncogenic infection by HPV, eventually leads to cervical carcinogenesis, associated by deregulation of specific pathways and protein expression at the intracellular and secretome level. Thus, secretome analysis can elucidate the biological mechanisms contributing to cervical cancer. In the present study we s...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20060
更新日期：2017-11-01 00:00:00
abstract：BACKGROUND/AIM:Pul-down assay is a popular in vitro method for identification of physical interactors of selected proteins. Here, for the first time, we compared three conventional variants of pull-down assay with the streptavidin-modified surface plasmon resonance (SPR) chips for the detection of PDZ and LIM domain pr...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20098
更新日期：2018-09-01 00:00:00
abstract：BACKGROUND/AIM:In a previous study we analyzed poliomyelitis incidence in US states, before the introduction of the polio vaccine. We noted an inverse correlation between polio incidence in US states, between 1937 and 1951, and colorectal cancer incidence (2005-2009). To further elucidate the role that poliovirus could...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20112
更新日期：2019-01-01 00:00:00
abstract：:Patients with pancreatic ductal adenocarcinoma have a dismall prognosis because at the time of diagnosis, in the vast majority of patients the tumor has already disseminated to distant organs and the therapeutic benefit of approved agents such as gemcitabine is limited. Therefore, the identification and preclinical an...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：10.21873/cgp.20149
更新日期：2019-11-01 00:00:00
abstract：BACKGROUND:Pancreatic cancer, has a very high mortality rate and requires novel molecular targets for diagnosis and therapy. Genetic association studies over databases offer an attractive starting point for gene discovery. MATERIALS AND METHODS:The National Center for Biotechnology Information (NCBI) Phenome Genome In...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：BACKGROUND/AIM:Dedifferentiated liposarcoma (DDLPS) is recalcitrant type of sarcoma. DDLPS has a low survival rate with high recurrence and metastasis. In the present study, we evaluated the efficacy of several drugs against doxorubicin-resistant DDLPS in a patient-derived orthotopic xenograft (PDOX) model for precisio...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20194
更新日期：2020-07-01 00:00:00
abstract：BACKGROUND/AIM:In breast cancer, initiation of carcinogenesis leads to epigenetic dysregulation, which can lead for example to the loss of the heterochromatin skeleton SUV39H1/H3K9me3/HP1 or the supposed secondary skeleton TIP60/P400/H4K12ac/BRD (2/4), which allows the maintenance of chromatin integrity and plasticity....
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20223
更新日期：2020-11-01 00:00:00
abstract：BACKGROUND:Identification and characterization of genes that are relevant to pancreatic cancer remains a priority for developing detection and diagnostic tests and identifying targets for treatment. MATERIALS AND METHODS:In order to discover relevant genes, we developed a microarray composed of 5763 pancreas and pancr...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2004-09-01 00:00:00
abstract：BACKGROUND:O-GlcNAcylation is a unique intracellular protein modification; however, few extracellular O-GlcNAc-modified proteins have been discovered. We have previously demonstrated that many cellular proteins were aberrant in O-GlcNAcylation in breast cancer tissues. In the present study, therefore, we investigated w...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-07-01 00:00:00
abstract：BACKGROUND:Metastasis associated gene-1 (MTA1), was initially discovered in aggressive human cancer cell lines and has been subsequently associated with the invasiveness and metastatic potential of cancer cells. MATERIALS AND METHODS:In the present study, we evaluated the expression levels of MTA1 in a cohort of human...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-05-01 00:00:00
abstract：:Breast cancer in men (MBC) is an uncommon malignancy and accounts for only 1% of all diagnosed breast cancers. By using genomic and transcriptomic approaches, researchers have been able to expand our insight into the genetic basis of breast cancer, by providing new biomarkers. We currently know that gene analysis by i...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:miRNAs play important roles in multiple biological processes, and deregulation has been linked to several human diseases, including cancer. Studying changes in miRNA expression in cancer development is commonly performed in vitro in human cancer cell lines using quantitative polymerase chain reaction (qPCR),...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:Although several microscopic techniques are available for separation of small groups of cells from surrounding tissue, the high energy concentrated into a small area, the easy control of beam position and the lack of direct contact with the material to be dissected make lasers the best option for a large scale microdi...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2006-05-01 00:00:00
abstract：BACKGROUND:Clinical observations indicate that tumour cells can acquire tolerance when an anticancer drug is administered repeatedly. In the present study, the gene expression in cisplatin-resistant cells was analysed to identify early changes in gene expression in the course of cisplatin exposure. MATERIALS AND METHO...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2006-11-01 00:00:00
abstract：BACKGROUND:Trastuzumab is a monoclonal antibody against HER2-positive breast cancer. Despite improving the natural history of the disease, there is a number of patients who are resistant to it, whereas all patients will eventually develop resistance and disease will progress. Inconsistent preclinical data show that the...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20105
更新日期：2018-11-01 00:00:00
abstract：BACKGROUND:Salivary gland cancer is a common type of head and neck cancer characterized by occasional deep invasion and lung metastasis. The precise role of sex steroid hormones in salivary gland cancer is unclear. To address this issue, we investigated whether the estrogen axis modulates salivary adenocarcinoma (SAC) ...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2016-07-01 00:00:00
abstract：AIM:To identify and study targets of microRNA biomarkers of glioblastoma survival across events (death and recurrence) and phases (life expectancy or post-diagnostic) using functional and network analyses. MATERIALS AND METHODS:microRNAs associated with glioblastoma survival within and across race, gender, recurrence,...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND/AIM:Medulloblastoma (MBL), an archetypal primitive neuroectodermal tumor of the cerebellum, is the most common pediatric central nervous system malignancy representing approximately 20% of all childhood brain tumors. Herein, we report on a new xenotransplantable tumor cell line, derived from a 6-year-old fem...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：10.21873/cgp.20037
更新日期：2017-07-01 00:00:00
abstract：:Breast cancer metastasis accounts for the majority of deaths from breast cancer. Detection of breast cancer metastasis at the earliest stage is important for the management and prediction of breast cancer progression. Emerging techniques using the analysis of circulating tumor cells show promising results in predictin...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章,评审
doi：
更新日期：2012-09-01 00:00:00
abstract：BACKGROUND:Metastasis-associated protein 1 (MTA1) plays an important role in tumourigenesis and progression of certain cancer types. In the current study, we analyzed the relationship between MTA1 expression and disease progression of colorectal cancer (CRC). MATERIALS AND METHODS:CRC tissues (n=93) and adjacent norma...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2015-11-01 00:00:00
abstract：:We recently reported that functional loss of p53 altered the responses of human HCT116 colon cancer cells to apoptosis triggers. To examine the molecular basis underlying the differential responses to drug treatment in the cancer cells, we performed a proteomic analysis in order to compare the protein expressions betw...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2012-03-01 00:00:00
abstract：:TACC2 is a member of the transforming acidic coiled-coil-containing protein family and is associated with the centrosome-spindle apparatus during cell cycling. The TACC2 gene is expressed in various splice forms predominantly in postmitotic tissues, including heart, muscle, kidney, and brain. Recent work has shown tha...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2010-03-01 00:00:00
abstract：:Human telomerase reverse transcriptase (hTERT) is overexpressed in prostate cancer. Estrogen plays a central role in the development of prostate cancer. hTERT activity has been shown to be increased after estrogen treatment. Although significant efforts have been made to understand the role of estrogen, the telomerase...
journal_title：Cancer genomics & proteomics
pub_type： 杂志文章
doi：
更新日期：2009-11-01 00:00:00
