The diagnostic value of the anti-PF4/heparin immunoassay high-dose heparin confirmatory test in cardiac surgery patients.

Abstract:

:There are limited and conflicting data on how a confirmatory step using high-dose heparin can improve diagnostic specificity of the antiplatelet factor 4/heparin enzyme immunoassay for heparin-induced thrombocytopenia (HIT). We investigated sera from a recently published study on cardiac surgery patients and found that only half of the sera that were heparin-induced platelet activation assay positive could be inhibited (optical density <40%) by high-dose heparin (100 IU/mL) in the enzyme immunoassay. More importantly, only 2 of the 3 patients with definite HIT were confirmatory test positive. Therefore, the high-dose heparin confirmatory test should be used with caution to exclude platelet-activating antiplatelet factor 4/heparin antibodies or clinical HIT.

journal_name

Anesth Analg

journal_title

Anesthesia and analgesia

authors

Selleng S,Schreier N,Wollert HG,Greinacher A

doi

10.1213/ANE.0b013e31820b5f39

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

774-6

issue

4

eissn

0003-2999

issn

1526-7598

pii

ANE.0b013e31820b5f39

journal_volume

112

pub_type

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