Polo-like kinase 2 (SNK/PLK2) is a novel epigenetically regulated gene in acute myeloid leukemia and myelodysplastic syndromes: genetic and epigenetic interactions.


:Polo-like kinase 2 (SNK/PLK2), a transcriptional target for wild-type p53 and is hypermethylated in a high percentage of multiple myeloma and B cell lymphomas patients. Given these data, we sought to study the methylation status of the specific gene in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), and to correlate it with clinical and genetic features. Using methylation-specific PCR MSP, we analyzed the methylation profile of 45 cases of AML and 43 cases of MDS. We also studied the distribution of MTHFR A1298C and MTHFR C677T polymorphisms and FLT3 mutations in AML patients and correlated the results with hypermethylation in the SNK/PLK2 CpG island. The SNK/PLK2 CpG island was hypermethylated in 68.9% and 88.4% of AML and MDS cases, respectively. Cases with hypermethylation had a trend towards more favorable overall survival (OS). There was no association between different MTHFR genotypes and susceptibility to develop AML. SNK/PLK2 hypermethylation combined with the MTHFR AA1298 genotype was associated with a tendency for a better OS. Similarly, patients with SNK/PLK2 hypermethylation combined with the MTHFR CT677 polymorphism had a better OS (HR = 0.34; p = 0.017). SNK/PLK2 methylation associated with unmutated FLT3 cases had a trend for better OS compared to patients with mutated FLT3 gene. SNK/PLK2 is a novel epigenetically regulated gene in AML and MDS, and methylation occurs at high frequency in both diseases. As such, SNK/PLK2 could represent a potential pathogenetic factor, although additional studies are necessary to verify its exact role in disease pathogenesis.


Ann Hematol


Annals of hematology


Benetatos L,Dasoula A,Hatzimichael E,Syed N,Voukelatou M,Dranitsaris G,Bourantas KL,Crook T




Has Abstract


2011-09-01 00:00:00












  • Enhanced expression of the nuclear envelope LAP2 transcriptional repressors in normal and malignant activated lymphocytes.

    abstract::Extensive research in recent years has broadened the functions of nuclear envelope proteins beyond simply stabilizing the nucleus architecture. Particularly, integral nuclear membrane proteins, such as the alternative spliced isoforms of lamina-associated polypeptide 2 (LAP2), have been shown to be important for the i...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Somech R,Gal-Yam EN,Shaklai S,Geller O,Amariglio N,Rechavi G,Simon AJ

    更新日期:2007-06-01 00:00:00

  • Lymphoma-like monoclonal B cell lymphocytosis in a patient population: biology, natural evolution, and differences from CLL-like clones.

    abstract::High-count monoclonal B cell lymphocytosis (MBL) with a chronic lymphocytic leukemia (CLL) phenotype is a well-known entity, featuring 1-4% annual risk of progression towards CLL requiring treatment. Lymphoma-like MBL (L-MBL), on the other hand, remains poorly defined and data regarding outcome are lacking. We retrosp...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Vander Meeren S,Heyrman B,Renmans W,Bakkus M,Maes B,De Raeve H,Schots R,Jochmans K

    更新日期:2018-07-01 00:00:00

  • Hereditary gene mutations in Korean patients with isolated erythrocytosis.

    abstract::Most cases of erythrocytosis occur secondary to chronic tissue hypoxia or as a clonal disease such as polycythemia vera with somatic mutations in the Janus kinase 2 (JAK2) gene. Rarely, erythrocytosis is caused by hereditary gene mutations. This study investigated hereditary gene mutations in 38 unrelated Korean patie...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Jang JH,Seo JY,Jang J,Jung CW,Lee KO,Kim SH,Kim HJ

    更新日期:2014-06-01 00:00:00

  • Significant prevalence of sickle cell disease in Southwest Germany: results from a birth cohort study indicate the necessity for newborn screening.

    abstract::Children with sickle cell disease (SCD) benefit from newborn screening, because life-threatening complications can be prevented by pre-symptomatic diagnosis. In Germany, the immigration of people from endemic countries is steadily growing. Comprehensive data about the epidemiology and prevalence of SCD in Germany are ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Kunz JB,Awad S,Happich M,Muckenthaler L,Lindner M,Gramer G,Okun JG,Hoffmann GF,Bruckner T,Muckenthaler MU,Kulozik AE

    更新日期:2016-02-01 00:00:00

  • Measuring holotranscobalamin II, an early indicator of negative vitamin B12 balance, by radioimmunoassay in patients with ischemic cerebrovascular disease.

    abstract::Circulating homocysteine is a risk factor of cardiovascular and cerebrovascular events. Hyperhomocysteinemia may be an early indicator for vitamin B12 disorders because cobalamin is a cofactor in the remethylation process of homocysteine. Serum holotranscobalamin (holoTC II) becomes decreased before the development of...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Serefhanoglu S,Aydogdu I,Kekilli E,Ilhan A,Kuku I

    更新日期:2008-05-01 00:00:00

  • High-resolution ultrasonography in gastrointestinal graft-versus-host disease.

    abstract::Gastrointestinal graft-versus-host disease (GVHD) is one of the main causes of therapy-related death after allogeneic hemopoietic stem cell transplantation. Early diagnosis and immediate treatment are probably essential for improving clinical outcome. High-resolution sonography allows the evaluation of single bowel wa...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Görg C,Wollenberg B,Beyer J,Stolte MS,Neubauer A

    更新日期:2005-01-01 00:00:00

  • Low-density lipoprotein (LDL) levels and risk of arterial occlusive events in chronic myeloid leukemia patients treated with nilotinib.

    abstract::Recommendations for dyslipidemia management aimed at reducing arterial occlusive events (AOEs) have been recently published. So far, no data have been reported on the management of dyslipidemia in chronic myeloid leukemia (CML) patients treated with nilotinib. We investigated 369 CML adult patients, stratified accordi...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Caocci G,Mulas O,Capodanno I,Bonifacio M,Annunziata M,Galimberti S,Luciano L,Tiribelli M,Martino B,Castagnetti F,Binotto G,Pregno P,Stagno F,Abruzzese E,Bocchia M,Gozzini A,Albano F,Fozza C,Luzi D,Efficace F,Simul

    更新日期:2021-01-03 00:00:00

  • Autologous peripheral blood stem cell transplant in patients previously diagnosed with invasive aspergillosis.

    abstract::Patients previously diagnosed with invasive aspergillosis (IA) have been considered to be at risk for relapse of mycosis during subsequent hematopoietic transplant. Even with prophylactic measures, reactivation of the infection occurs in 29% of patients undergoing bone marrow transplantation (BMT). A period of neutrop...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Sevilla J,Hernandez-Maraver D,Aguado MJ,Ojeda E,Morado M,Hernandez-Navarro F

    更新日期:2001-08-01 00:00:00

  • The role of cytokines and hematopoietic growth factors in the autocrine/paracrine regulation of inducible hematopoiesis.

    abstract::To elucidate the role of hematopoietic growth factors (HGFs) and other cytokines in the autocrine or paracrine regulation of inducible hematopoiesis we studied cytokine gene expression in the bone marrow (BM) of patients after myelosuppressive treatment. Furthermore, we studied the cytokine gene expression profile in ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Cluitmans FH,Esendam BH,Veenhof WF,Landegent JE,Willemze R,Falkenburg JH

    更新日期:1997-07-01 00:00:00

  • Interferon alpha in the treatment of polycythemia vera.

    abstract::Interferon alpha (IFN) inhibits the growth of the abnormal clone in patients with myeloproliferative disorders, leading to a reduction of the clinical and laboratory signs of the pathologic myeloproliferation. The therapeutic efficacy of IFN in polycythemia vera (PV) is demonstrated by the summarized treatment results...

    journal_title:Annals of hematology

    pub_type: 杂志文章,评审


    authors: Lengfelder E,Berger U,Hehlmann R

    更新日期:2000-03-01 00:00:00

  • Nitric oxide metabolites and arginase I levels in β-thalassemic patients: an Egyptian study.

    abstract::Stored red blood cells become deficient in nitric oxide that limits their ability to transfer oxygen to tissues that need it. The aims of this study are to assess the endogenous nitric oxide metabolites (NOx) and arginase I levels in transfusion-dependent β-thalassemic patients; to compare these levels in patients tra...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: El-Hady SB,Farahat MH,Atfy M,Elhady MA

    更新日期:2012-08-01 00:00:00

  • Multiple myeloma: effect of daily dichloromethylene bisphosphonate on skeletal complications.

    abstract::In 1989, a prospective randomized multicenter study was initiated in order to determine the safety and efficacy of oral clodronate in myeloma patients. The primary objective of this long-term trial is to evaluate whether supportive clodronate is able to prevent or retard the progression of bone disease and reduce the ...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Clemens MR,Fessele K,Heim ME

    更新日期:1993-03-01 00:00:00

  • Exacerbation of antiphospholipid antibody syndrome after treatment of localized cancer: a report of two cases.

    abstract::Patients with malignancy often present with a variety of coagulation abnormalities which may ultimately lead to recurrent arterial and venous thromboses. Recently the presence of antiphospholipid antibodies in cancer patients has been proposed as one of the potential mechanisms promoting hypercoagulability. Here we re...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Langer F,Eifrig B,Marx G,Stork A,Hegewisch-Becker S,Hossfeld DK

    更新日期:2002-12-01 00:00:00

  • Long-term outcomes of 172 children with severe aplastic anemia treated with rabbit antithymocyte globulin and cyclosporine.

    abstract::This study retrospectively analyzed the clinical outcome of 172 children with newly diagnosed severe aplastic anemia (SAA) between January 2008 and April 2018, who received rabbit antithymocyte globulin (ATG) and cyclosporine (CsA) as first-line treatment. The median age at diagnosis was 5 years (range, 1-14). The ove...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Lan Y,Chang L,Yi M,Cai Y,Feng J,Ren Y,Liu C,Chen X,Wang S,Guo Y,Zhang A,Liu L,Zhang J,Zhu X

    更新日期:2021-01-01 00:00:00

  • Allogeneic bone marrow transplantation compared to peripheral blood stem cell transplantation for the treatment of hematologic malignancies: a meta-analysis based on time-to-event data from randomized controlled trials.

    abstract::Controversy remains regarding the transplant outcomes of human leukocyte antigen-identical related bone marrow transplantation (BMT) and peripheral blood stem cell transplantation (PBSCT) for the treatment of patients with hematological malignancies. To provide an estimate of the effect of BMT and PBSCT on clinical ou...

    journal_title:Annals of hematology

    pub_type: 杂志文章,meta分析


    authors: Chang YJ,Weng CL,Sun LX,Zhao YT

    更新日期:2012-03-01 00:00:00

  • Individual outcome prediction for myelodysplastic syndrome (MDS) and secondary acute myeloid leukemia from MDS after allogeneic hematopoietic cell transplantation.

    abstract::We integrated molecular data with available prognostic factors in patients undergoing allogeneic hematopoietic cell transplantation (alloHCT) for myelodysplastic syndrome (MDS) or secondary acute myeloid leukemia (sAML) from MDS to evaluate their impact on prognosis. Three hundred four patients were sequenced for muta...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Heuser M,Gabdoulline R,Löffeld P,Dobbernack V,Kreimeyer H,Pankratz M,Flintrop M,Liebich A,Klesse S,Panagiota V,Stadler M,Wichmann M,Shahswar R,Platzbecker U,Thiede C,Schroeder T,Kobbe G,Geffers R,Schlegelberger B,Gö

    更新日期:2017-08-01 00:00:00

  • Specific cutaneous infiltrate caused by Staphylococcus aureus in a patient with chronic myelomonocytic leukemia.

    abstract::We present a patient with chronic myelomonocytic leukemia who showed disseminated papules and nodules. Arguments in favor of leukemia cutis are the clinical appearance, the cyclic pattern with which the lesions appeared and disappeared, and the histologic features. The lesions reproducibly responded to treatment with ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Wintzen M,Kluin PM,den Ottolander GJ

    更新日期:2000-07-01 00:00:00

  • Prognostic factors of early outcome in pediatric hemophagocytic lymphohistiocytosis: an analysis of 116 cases.

    abstract::Early mortality remains a major challenge for the treatment of hemophagocytic lymphohistiocytosis (HLH), which warrants the need for prompt risk stratification in the early phase of the disease. We retrospectively analyzed clinical features of a cohort of pediatric patients managed at a tertiary hospital in southern C...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Bin Q,Gao JH,Luo JM

    更新日期:2016-09-01 00:00:00

  • Prognostic impact of intraocular involvement in primary CNS lymphoma: experience from the G-PCNSL-SG1 trial.

    abstract::The impact of intraocular involvement (IOL) in primary CNS lymphoma (PCNSL) has not been sufficiently evaluated. Here, we present the analysis of IOL in the only completed randomized phase III trial in PCNSL. The G-PCNSL-SG1 study evaluated the role of whole-brain radiotherapy in primary therapy of PCNSL. Data of the ...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Kreher S,Strehlow F,Martus P,Roth P,Hertenstein B,Röth A,Birnbaum T,Griesinger F,Rauch M,Kanz L,Thiel E,Weller M,Korfel A

    更新日期:2015-03-01 00:00:00

  • Biological activity of lenalidomide in myelodysplastic syndromes with del5q: results of gene expression profiling from a multicenter phase II study.

    abstract::In vitro studies suggest that haploinsufficiency is involved in the pathogenesis of myelodysplastic syndromes (MDS). In patients with del5q cytogenetic abnormality, RPS-14 and microRNAs (miRNAs) play a major role. In a multicenter phase II single-arm trial with lenalidomide in anemic primary del5q MDS patients with lo...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Oliva EN,Cuzzola M,Aloe Spiriti MA,Poloni A,Laganà C,Rigolino C,Morabito F,Galimberti S,Ghio R,Cortelezzi A,Palumbo GA,Sanpaolo G,Finelli C,Ricco A,Volpe A,Rodà F,Breccia M,Alimena G,Nobile F,Latagliata R

    更新日期:2013-01-01 00:00:00

  • Prothrombotic polymorphisms, mutations, and their association with pediatric non-cardioembolic stroke in Asian-Indian patients.

    abstract::Genes involved in the hemostatic mechanism are logical candidate genes for association studies in prothrombotic conditions such as stroke. Since the underlying etiology in pediatric strokes is different than adults, looking for genetic causes would be the logical thing to do in the pediatric stroke population. Fifty-e...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Biswas A,Tiwari AK,Ranjan R,Meena A,Akhter MS,Yadav BK,Behari M,Saxena R

    更新日期:2009-05-01 00:00:00

  • Plasma concentrations of total/free and functional protein S are not decreased in systemic lupus erythematosus patients with lupus anticoagulant and/or antiphospholipid antibodies.

    abstract::We conducted an investigation to clarify whether or not the levels of total, free, and functional protein S and C4-binding protein (C4bp) in plasma are decreased in systemic lupus erythematosus (SLE) patients, especially those with antiphospholipid antibody (aPL), which is known to be a causative factor of such compli...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Matsuda J,Gohchi K,Gotoh M,Tsukamoto M,Saitoh N

    更新日期:1994-12-01 00:00:00

  • Prevalence of increased osmotic fragility of erythrocytes in German blood donors: screening using a modified glycerol lysis test.

    abstract::We screened for increased osmotic fragility of erythrocytes in 1464 healthy German blood donors. The osmotic fragility was determined by an acidified glycerol lysis test (AGLT) using glycerol-sodium phosphate-buffered NaCl solution. Since the original test described by Zanella et al. [23] showed only low specificity f...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Eber SW,Pekrun A,Neufeldt A,Schröter W

    更新日期:1992-02-01 00:00:00

  • Long-term outcomes of autologous hematopoietic stem cell transplantation with reduced-intensity conditioning in multiple sclerosis: physician's and patient's perspectives.

    abstract::High-dose immunosuppressive therapy (HDIT) with autologous hematopoietic stem cell transplantation (AHSCT) is a promising approach to treatment of multiple sclerosis (MS) patients. In this paper, we present the long-term outcomes of a prospective single-center study with the analysis of the safety and efficacy of HDIT...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Shevchenko JL,Kuznetsov AN,Ionova TI,Melnichenko VY,Fedorenko DA,Kurbatova KA,Gorodokin GI,Novik AA

    更新日期:2015-07-01 00:00:00

  • Aerosol amphotericin B inhalations for prevention of invasive pulmonary aspergillosis in neutropenic cancer patients.

    abstract::To determine the value of aerosol amphotericin B inhalations for prevention of invasive pulmonary aspergillosis (IPA), we initiated a prospective randomized multicenter trial. The scheduled intent-to-treat interim analysis included 115 patients (30%) with prolonged neutropenia after chemotherapy for acute myeloid leuk...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Behre GF,Schwartz S,Lenz K,Ludwig WD,Wandt H,Schilling E,Heinemann V,Link H,Trittin A,Boenisch O

    更新日期:1995-12-01 00:00:00

  • Thrombotic thrombocytopenic purpura in a patient with sickle cell crisis.

    abstract::The combination of sickle cell disease crisis and thrombotic thrombocytopenic purpura has been described only a few times. Here we present the case of a patient with a hemolytic crisis due to sickle cell disease complicated by thrombotic thrombocytopenic purpura. We also review the cases previously reported and compar...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Bolaños-Meade J,Keung YK,López-Arvizu C,Florendo R,Cobos E

    更新日期:1999-12-01 00:00:00

  • Failure of repeated courses of high-dose intravenous immunoglobulin to induce stable remission in patients with chronic idiopathic thrombocytopenic purpura.

    abstract::Repeated courses of HD IVIg are reported to induce stable remission in a significant proportion of adults with chronic refractory ITP. We have treated 14 such patients obtaining a remission rate quite comparable to the 5-10% of spontaneous remission. ...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Schiavotto C,Ruggeri M,Rodeghiero F

    更新日期:1995-02-01 00:00:00

  • Allogeneic bone marrow transplantation for refractory mantle cell lymphoma.

    abstract::We report about a 28-year-old woman with relapsed mantle cell lymphoma (MCL, centrocytic lymphoma according to the Kiel classification) refractory to salvage chemotherapy. The patient underwent allogeneic bone marrow transplantation from a HLA-identical brother after myeloablative chemotherapy consisting of busulfan, ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Kröger N,Hoffknecht M,Krüger W,Zeller W,Renges H,Stute N,Zschaber R,Zander AR

    更新日期:2000-10-01 00:00:00

  • New prognostic model for extranodal natural killer/T cell lymphoma, nasal type.

    abstract::Extranodal natural killer/T cell lymphoma, nasal type (ENKTL) is an aggressive disease with a poor prognosis, requiring risk stratification in affected patients. We designed a new prognostic model specifically for ENKTL to identify high-risk patients who need more aggressive therapy. We retrospectively reviewed 158 pa...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Cai Q,Luo X,Zhang G,Huang H,Huang H,Lin T,Jiang W,Xia Z,Young KH

    更新日期:2014-09-01 00:00:00

  • Haemoglobinopathies with high oxygen affinity. Experience of Erythropathology Cooperative Spanish Group.

    abstract::Haemoglobinopathies are the world's most frequently found monogenic disorders. In the cases with high oxygen affinity, the decrease in the liberation of the oxygen determines a secondary erythrocytosis. In this work, we present 17 unrelated families of Caucasian race and of Spanish origin, with ten variants of haemogl...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: González Fernández FA,Villegas A,Ropero P,Carreño MD,Anguita E,Polo M,Pascual A,Henández A

    更新日期:2009-03-01 00:00:00