Polo-like kinase 2 (SNK/PLK2) is a novel epigenetically regulated gene in acute myeloid leukemia and myelodysplastic syndromes: genetic and epigenetic interactions.


:Polo-like kinase 2 (SNK/PLK2), a transcriptional target for wild-type p53 and is hypermethylated in a high percentage of multiple myeloma and B cell lymphomas patients. Given these data, we sought to study the methylation status of the specific gene in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), and to correlate it with clinical and genetic features. Using methylation-specific PCR MSP, we analyzed the methylation profile of 45 cases of AML and 43 cases of MDS. We also studied the distribution of MTHFR A1298C and MTHFR C677T polymorphisms and FLT3 mutations in AML patients and correlated the results with hypermethylation in the SNK/PLK2 CpG island. The SNK/PLK2 CpG island was hypermethylated in 68.9% and 88.4% of AML and MDS cases, respectively. Cases with hypermethylation had a trend towards more favorable overall survival (OS). There was no association between different MTHFR genotypes and susceptibility to develop AML. SNK/PLK2 hypermethylation combined with the MTHFR AA1298 genotype was associated with a tendency for a better OS. Similarly, patients with SNK/PLK2 hypermethylation combined with the MTHFR CT677 polymorphism had a better OS (HR = 0.34; p = 0.017). SNK/PLK2 methylation associated with unmutated FLT3 cases had a trend for better OS compared to patients with mutated FLT3 gene. SNK/PLK2 is a novel epigenetically regulated gene in AML and MDS, and methylation occurs at high frequency in both diseases. As such, SNK/PLK2 could represent a potential pathogenetic factor, although additional studies are necessary to verify its exact role in disease pathogenesis.


Ann Hematol


Annals of hematology


Benetatos L,Dasoula A,Hatzimichael E,Syed N,Voukelatou M,Dranitsaris G,Bourantas KL,Crook T




Has Abstract


2011-09-01 00:00:00












  • Ruxolitinib in clinical practice for primary and secondary myelofibrosis: an analysis of safety and efficacy of Gruppo Laziale of Ph-negative MPN.

    abstract::Ruxolitinib, a JAK1 and JAK2 inhibitor, has been tested and approved for the treatment of primary and secondary myelofibrosis (MF). Aim of our study is to report safety and efficacy of ruxolitinib in 98 patients affected by MF treated outside clinical trials and collected and treated consecutively by the Lazio Coopera...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Breccia M,Andriani A,Montanaro M,Abruzzese E,Buccisano F,Cedrone M,Centra A,Villivà N,Celesti F,Trawinska MM,Massaro F,Di Veroli A,Anaclerico B,Colafigli G,Molica M,Spadea A,Petriccione L,Cimino G,Latagliata R

    更新日期:2017-03-01 00:00:00

  • Influence of body mass index on survival in indolent and mantle cell lymphomas: analysis of the StiL NHL1 trial.

    abstract::Obesity is a well-known risk factor for the development of cancer, but its influence on the course of disease is still controversial. We investigated the influence of body mass index (BMI) on overall survival (OS) in 502 patients with indolent non-Hodgkin's lymphoma or mantle cell lymphoma in a subgroup analysis of th...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Weiss L,Melchardt T,Egle A,Hopfinger G,Hackl H,Greil R,Barth J,Rummel M

    更新日期:2017-07-01 00:00:00

  • Platelet number and graft function predict intensive care survival in allogeneic stem cell transplantation patients.

    abstract::Despite significant advances in the treatment of complications requiring intensive care unit (ICU) admission, ICU mortality remains high for patients after allogeneic stem cell transplantation. We evaluated the role of thrombocytopenia and poor graft function in allogeneic stem cell recipients receiving ICU treatments...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Turki AT,Lamm W,Schmitt C,Bayraktar E,Alashkar F,Metzenmacher M,Wohlfarth P,Beelen DW,Liebregts T

    更新日期:2019-02-01 00:00:00

  • Mosaicism in Fanconi anemia: concise review and evaluation of published cases with focus on clinical course of blood count normalization.

    abstract::Fanconi anemia (FA) is a DNA repair disorder resulting from mutations in genes encoding for FA DNA repair complex components and is characterized by variable congenital abnormalities, bone marrow failure (BMF), and high incidences of malignancies. FA mosaicism arises from reversion or other compensatory mutations in h...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Nicoletti E,Rao G,Bueren JA,Río P,Navarro S,Surrallés J,Choi G,Schwartz JD

    更新日期:2020-05-01 00:00:00

  • Blood B, T, CD4+ and CD8+ lymphocytes in female Wistar rats.

    abstract::We have established reference values of peripheral blood lymphocyte subsets in healthy female Wistar rats under highly standardized conditions. Using monoclonal antibodies and flow cytometry, T lymphocytes (OX19+), B lymphocytes (OX6+ and anti-Ig+), T-helper/inducer (W3/25+), and T-suppressor/cytotoxic subsets (OX8+) ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


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    更新日期:1993-09-01 00:00:00

  • Rituximab therapy for hairy cell leukemia: a retrospective study of 41 cases.

    abstract::The purine analogs (PAs) cladribine and pentostatin have transformed the prognosis of hairy cell leukemia (HCL). However, some patients still relapse after PAs, or fail to reach an optimal response, and new agents are needed to further improve treatment outcome. We retrospectively studied 41 HCL patients from 10 cente...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Leclerc M,Suarez F,Noël MP,Vekhoff A,Troussard X,Claisse JF,Thieblemont C,Maloisel F,Beguin Y,Tamburini J,Barbe C,Delmer A

    更新日期:2015-01-01 00:00:00

  • Polymorphisms of interleukin-1 gene complex, IL6 and tumour necrosis factor genes in chronic idiopathic neutropenia of adults.

    abstract::Chronic idiopathic neutropenia of adults (CINA) is a granulocytic disorder characterised by the "unexplained" decrease in the number of circulating neutrophils. Serum inflammatory cytokines and chemokines are increased in CINA. In addition, cytokines gene polymorphisms are associated with increased levels of respectiv...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Addas-Carvalho M,de Paula EV,Lima CS,Saad ST

    更新日期:2005-10-01 00:00:00

  • Prothrombotic polymorphisms, mutations, and their association with pediatric non-cardioembolic stroke in Asian-Indian patients.

    abstract::Genes involved in the hemostatic mechanism are logical candidate genes for association studies in prothrombotic conditions such as stroke. Since the underlying etiology in pediatric strokes is different than adults, looking for genetic causes would be the logical thing to do in the pediatric stroke population. Fifty-e...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Biswas A,Tiwari AK,Ranjan R,Meena A,Akhter MS,Yadav BK,Behari M,Saxena R

    更新日期:2009-05-01 00:00:00

  • Liver fibrosis alleviation after co-transplantation of hematopoietic stem cells with mesenchymal stem cells in patients with thalassemia major.

    abstract::The aims of this study are to determine the replacement rate of damaged hepatocytes by donor-derived cells in sex-mismatched recipient patients with thalassemia major and to determine whether co-transplantation of mesenchymal stem cells and hematopoietic stem cells (HSCs) can alleviate liver fibrosis. Ten sex-mismatch...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Ghavamzadeh A,Sotoudeh M,Hashemi Taheri AP,Alimoghaddam K,Pashaiefar H,Jalili M,Shahi F,Jahani M,Yaghmaie M

    更新日期:2018-02-01 00:00:00

  • Polymorphisms in cytokine genes as prognostic markers in diffuse large B cell lymphoma patients treated with (R)-CHOP.

    abstract::To investigate whether cytokine genetic polymorphisms influence the outcome of diffuse large B cell lymphoma (DLBCL), we tested 337 consecutive DLBCL treated with CHOP or rituximab-CHOP (R-CHOP) from interleukin 10 (IL10), Bcl-2, and tumor necrosis factor (TNF)-α polymorphisms. Patients who carried the IL10 rs1800871 ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Liu D,Wang Y,Dong M,Guan S,Wang Y,Sun H,Wu N,Li S,Bai J,Chen F,Sun D,Jin Y

    更新日期:2017-02-01 00:00:00

  • Morphologic and phenotypic changes of the leukemic cells in a case of marginal zone B-cell lymphoma.

    abstract::A particular case of marginal zone B-cell lymphoma (MZBCL) presenting with leukemic lymphocytes is reported. In the present observation, the leukemic cells not only displayed a remarkable morphological fluctuation but also had an unusual phenotype, changing with time. These phenotypic features, which have been functio...

    journal_title:Annals of hematology

    pub_type: 杂志文章


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    更新日期:1997-03-01 00:00:00

  • Emerging resistant bacteria strains in bloodstream infections of acute leukaemia patients: results of a prospective study by the Rete Ematologica Lombarda (Rel).

    abstract::Multiresistant bacterial infections are a potentially life-threatening condition in acute leukaemia (AL) patients. We aimed to better define the very recent epidemiology and outcome of bloodstream infections (BSIs) in a real-life setting. We prospectively collected all consecutive febrile/infectious episodes occurring...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Cattaneo C,Zappasodi P,Mancini V,Annaloro C,Pavesi F,Skert C,Ferrario A,Todisco E,Saccà V,Verga L,Passi A,Da Vià M,Ferrari S,Mometto G,Petullà M,Nosari A,Rossi G

    更新日期:2016-12-01 00:00:00

  • Changes in bone marrow morphology in adults receiving romiplostim for the treatment of thrombocytopenia associated with primary immune thrombocytopenia.

    abstract::The effects of romiplostim on bone marrow morphology were evaluated in adults with immune thrombocytopenia (ITP). Patients with platelet counts <50 × 10(9)/L, ≥1 prior ITP therapies, and no collagen at baseline received weekly subcutaneous romiplostim starting at 1 μg/kg, adjusted to maintain platelet counts between 5...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Janssens A,Rodeghiero F,Anderson D,Chong BH,Boda Z,Pabinger I,Červinek L,Terrell DR,Wang X,Franklin J

    更新日期:2016-06-01 00:00:00

  • CT-guided needle biopsy performed with modified coaxial technique in patients with refractory or recurrent lymphomas.

    abstract::The aim of this study was to evaluate the role of computed tomography (CT)-guided core needle biopsy (CNB) performed by modified coaxial technique as an alternative tool to surgical biopsy in patients with refractory or recurrent lymphomas. Between May 2005 and May 2012, 57 CT-guided CNB of deep lesions were performed...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Gaudio F,Pedote P,Ferrante A,Perrone T,Ingravallo G,Ianora AA,Angelelli G,Specchia G

    更新日期:2014-09-01 00:00:00

  • X-linked thrombocytopenia and Wiskott-Aldrich syndrome: similar regional assignment but distinct X-inactivation pattern in carriers.

    abstract::While inherited X-linked (XL) isolated thrombocytopenia is a mild condition, the Wiskott-Aldrich syndrome (WAS) associates severe thrombocytopenia with an immunodeficiency component and has a poor prognosis. Whether these conditions correspond to separate genetic entities or to different mutations of the same gene(s) ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: De Saint-Basile G,Schlegel N,Caniglia M,Le Deist F,Kaplan C,Lecompte T,Piller F,Fischer A,Griscelli C

    更新日期:1991-08-01 00:00:00

  • Enhanced expression of the nuclear envelope LAP2 transcriptional repressors in normal and malignant activated lymphocytes.

    abstract::Extensive research in recent years has broadened the functions of nuclear envelope proteins beyond simply stabilizing the nucleus architecture. Particularly, integral nuclear membrane proteins, such as the alternative spliced isoforms of lamina-associated polypeptide 2 (LAP2), have been shown to be important for the i...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Somech R,Gal-Yam EN,Shaklai S,Geller O,Amariglio N,Rechavi G,Simon AJ

    更新日期:2007-06-01 00:00:00

  • Regulation of FLT3 and its ligand in normal hematopoietic progenitor cells.

    abstract::FLT3 and its ligand (FL) are one of the regulators of normal hematopoiesis. Ligand-independent activation of FLT3 occurs in about 30% of acute myeloid leukemia cases and is one goal for selectively targeted therapies. However, the function of FLT3/FL in the regulation of non-malignant immature hematopoietic cells is p...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Weisel KC,Yildirim S,Schweikle E,Kanz L,Möhle R

    更新日期:2009-03-01 00:00:00

  • Long-term outcome of follicular low-grade lymphoma. A report of 91 patients.

    abstract::We retrospectively analyzed overall survival and survival after progression in 91 patients with low-grade follicular lymphoma (LGFL). Histological subtype was B in 75 patients and C in 16 patients. Twelve patients with localized disease received involved-field radiotherapy; seven patients without bulky disease had no ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Morel P,Dupriez B,Plantier-Colcher I,Gosselin B,Declercq C,Pollet JP,Bauters F

    更新日期:1993-06-01 00:00:00

  • A case of multicentric Castleman's disease associated with advanced systemic amyloidosis treated with chemotherapy and anti-CD20 monoclonal antibody.

    abstract::Multicentric Castleman's disease (MCD) is a rare systemic lymphoproliferative disorder with too few patient series reported in the literature to have a clear idea about the etiology, outcome and the best treatment available. Systemic reactive amyloidosis is a very rare complication of MCD and its presence worsens the ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Gholam D,Vantelon JM,Al-Jijakli A,Bourhis JH

    更新日期:2003-12-01 00:00:00

  • The effects of air quality on haematological and clinical parameters in children with sickle cell anaemia.

    abstract::Sickle cell anaemia (SCA; HbSS) is characterised by its clinical variability, which is only partly explained by known genetic factors. Environmental factors are known to contribute to acute problems but their importance in chronic complications has not been analysed. We have studied 93 children with SCA in a single in...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Mittal H,Roberts L,Fuller GW,O'Driscoll S,Dick MC,Height SE,Thein SL,Rees DC

    更新日期:2009-06-01 00:00:00

  • Immunophenotyping of B lymphocytes by multiparametric flow cytometry in bone marrow aspirates of healthy adults.

    abstract::Establishing reference ranges by multiparametric immunophenotyping of mature B cells in bone marrow of healthy adults is of interest because the detection of bone marrow infiltration and persistence of light chain restriction, as well as discrimination between reactive and malignant lymphocytes are important applicati...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Höffkes HG,Schmidtke G,Schmücker U,Uppenkamp M,Brittinger G

    更新日期:1995-09-01 00:00:00

  • Erythropoiesis and serum sVCAM-1 levels in adults with sickle cell disease.

    abstract::Sickle cell patients are characterized by stress erythropoiesis involving cytokines, growth factors, and adhesion molecules. We set out to determine whether serum soluble vascular cell adhesion molecule-1 (sVCAM-1) levels, which are inversely related to red blood cell counts in sickle cell disease (SCD), reflect eryth...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Duits AJ,Rojer RA,van Endt T,MacGillavry MR,ten Cate H,Brandjes DP,Schnog JB

    更新日期:2003-03-01 00:00:00

  • Possible effect of medroxyprogesterone acetate (MPA) in lymphoid blast crisis of chronic myelogenous leukemia.

    abstract::A patient with a lymphoid blast crisis of a chronic myelogenous leukemia (CML) was treated with vindesine, vincristine and prednisone. Blasts disappeared from the peripheral blood but persisted at a level of 60% in the bone marrow. After 5 weeks of continuous therapy, the patient became thrombopenic, and 2 weeks later...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Fink M

    更新日期:1994-02-01 00:00:00

  • In vivo induction of HLA molecules in patients with myeloproliferative syndrome during IFN alpha treatment.

    abstract::Eighteen patients with myeloproliferative syndrome (14 with chronic myeloid leukemia, four with essential thrombocytosis) were investigated for modulation of HLA antigens on peripheral blood lymphocytes, monocytes, and hematopoietic precursors during IFN alpha therapy as a sign of potentially increased immune recognit...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Müller CA,Walz J,Zinser R,Bühring HJ,Steinke B,Schmidt H

    更新日期:1991-11-01 00:00:00

  • The role of cytokines and hematopoietic growth factors in the autocrine/paracrine regulation of inducible hematopoiesis.

    abstract::To elucidate the role of hematopoietic growth factors (HGFs) and other cytokines in the autocrine or paracrine regulation of inducible hematopoiesis we studied cytokine gene expression in the bone marrow (BM) of patients after myelosuppressive treatment. Furthermore, we studied the cytokine gene expression profile in ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Cluitmans FH,Esendam BH,Veenhof WF,Landegent JE,Willemze R,Falkenburg JH

    更新日期:1997-07-01 00:00:00

  • Fine needle aspiration and core needle biopsy in the diagnosis of lymphadenopathy of unknown aetiology.

    abstract::The clarification of enlarged lymph nodes is a common issue in clinical routine. By now, open surgery with complete lymph node extirpation, followed by histopathology, is considered as standard. We investigated the value of fine needle aspiration (FNA) and core needle biopsy (CNB) when supporting the conventional morp...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Metzgeroth G,Schneider S,Walz C,Reiter S,Hofmann WK,Marx A,Hastka J

    更新日期:2012-09-01 00:00:00

  • Bloodstream infections with gram-negative organisms and the impact of multidrug resistance in patients with hematological malignancies.

    abstract::Infections and especially blood stream infections (BSI) with gram-negative bacteria (GNB) represent a major threat for patients with hematological diseases undergoing chemotherapy and mainly contribute to morbidity and mortality. In this retrospective single-center study, we analyzed the impact of BSI with different g...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Scheich S,Weber S,Reinheimer C,Wichelhaus TA,Hogardt M,Kempf VAJ,Kessel J,Serve H,Steffen B

    更新日期:2018-11-01 00:00:00

  • Sudden onset of EDTA-dependent pseudothrombocytopenia after therapy with the glycoprotein IIb/IIIa antagonist c7E3 Fab.

    abstract::The development of thrombocytopenia following exposure to the platelet glycoprotein (GP) IIb/ IIIa receptor antagonist abciximab (c7E3 Fab, ReoPro) is associated with adverse clinical outcome and excessive bleeding. Pseudothrombocytopenia is an important differential diagnosis in sudden onset of thrombocytopenia in a ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Stiegler H,Fischer Y,Steiner S,Strauer BE,Reinauer H

    更新日期:2000-03-01 00:00:00

  • Granulocyte colony-stimulating factor shortens duration of critical neutropenia and prolongs disease-free survival after sequential high-dose cytosine arabinoside and mitoxantrone (S-HAM) salvage therapy for refractory and relapsed acute myeloid leukemia.

    abstract::Patients with primary refractory or relapsed acute myeloid leukemia (AML) who undergo intensive salvage chemotherapy carry a high risk of treatment failure due to infectious complications and early relapses. The study presented here assessed the effect of granulocyte colony-stimulating factor (G-CSF) on the duration o...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Kern W,Aul C,Maschmeyer G,Kuse R,Kerkhoff A,Grote-Metke A,Eimermacher H,Kubica U,Wörmann B,Büchner T,Hiddemann W

    更新日期:1998-09-01 00:00:00

  • Prognostic impact of bleomycin-induced pneumonitis on the outcome of Hodgkin's lymphoma.

    abstract::Bleomycin-induced pneumonitis (BIP) has been well described in Hodgkin's lymphoma (HL) patients. The impact of BIP on patients uniformly treated with adriamycin, bleomycin, vinblastine, and dacarbazine (ABVD) is not clear; previous studies have included patients treated with both ABVD and hybrid regimens. We reviewed ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Ngeow J,Tan IB,Kanesvaran R,Tan HC,Tao M,Quek R,Lim ST

    更新日期:2011-01-01 00:00:00