Abstract：
:Temperature and pH effects were studied for stability, structural organization, fluidity and permeability of vesicles from a polar lipid methanol fraction isolated from the Aeropyrum pernix. We determined the permeability of C(25,25) liposomes using fluorescence intensity of released calcein. At pH 7.0 and 9.0, and from 85°C to 98°C, only 10% of entrapped calcein was released. After 10 h at 90°C, calcein release reached 27%, independent of pH. Fluorescence anisotropy measurements of hydrophobic probe 1,6-diphenyl-1,3,5-hexatriene revealed gradual changes up to 60°C. At higher temperatures, the anisotropy did not change significantly. Fluorescence alone did not provide detailed and direct structural information about these C(25,25) liposomes, so we used electron paramagnetic resonance spectroscopy (EPR) and differential scanning calorimetry (DSC). From EPR spectra, mean membrane fluidity determined according to maximal hyperfine splitting and empirical correlation times showed continuous increases with temperature. Computer simulation of EPR spectra showed heterogeneous membranes of these C(25,25) liposomes: at low temperatures, they showed three types of membrane regions characterized by different motional modes. Above 65°C, the membrane becomes homogeneous with only one fluid-like region. DSC thermograms of C(25,25) liposomes reveal a very broad and endothermic transition in the temperature range from 0°C to 40°C.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Gmajner D,Ota A,Sentjurc M,Ulrih NPdoi
10.1016/j.chemphyslip.2011.01.005subject
Has Abstractpub_date
2011-03-01 00:00:00pages
236-45issue
3eissn
0009-3084issn
1873-2941pii
S0009-3084(11)00018-1journal_volume
164pub_type
杂志文章abstract：:A myriad of health benefits including the prevention of cancer and heart disease accompanies consumption of polyunsaturated fatty acids (PUFA). Of special importance is the omega-3-PUFA docosahexaenoic acid (DHA), with 22 carbons and six double bonds that constitute the most highly unsaturated fatty acid naturally occ...
journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/s0009-3084(01)00130-x
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/j.chemphyslip.2010.07.001
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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更新日期：1994-01-01 00:00:00
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/j.chemphyslip.2009.10.007
更新日期：2010-01-01 00:00:00
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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doi：10.1016/0009-3084(94)90066-3
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/0009-3084(79)90111-7
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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doi：10.1016/s0009-3084(98)00029-2
更新日期：1998-06-01 00:00:00
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
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doi：10.1016/0009-3084(77)90003-2
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/0009-3084(83)90027-0
更新日期：1983-11-01 00:00:00
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/j.chemphyslip.2008.04.009
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journal_title：Chemistry and physics of lipids
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章,评审
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更新日期：1987-07-01 00:00:00
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journal_title：Chemistry and physics of lipids
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doi：10.1016/0009-3084(91)90026-8
更新日期：1991-10-01 00:00:00
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/0009-3084(94)90128-7
更新日期：1994-01-01 00:00:00
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journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/j.chemphyslip.2018.03.001
更新日期：2018-07-01 00:00:00
abstract：:The decomposition of hydrogen peroxide catalyzed by catalase entrapped in the pool of dipalmitoylphosphatidyl choline unilamellar liposomes has been studied. The rate of the process was evaluated by following the production of oxygen as a function of time. Under the experimental conditions employed the rate of oxygen ...
journal_title：Chemistry and physics of lipids
pub_type： 杂志文章
doi：10.1016/j.chemphyslip.2012.07.001
更新日期：2012-09-01 00:00:00
