Abstract:
:A method using liquid chromatography - atmospheric pressure chemical ionisation mass spectrometry was evaluated for determining the molecular species composition of phospholipids (phosphatidylcholines from soybean, egg yolk and bovine liver) after conversion to diacylglycerol nicotinate derivatives. The structures could be deduced from pseudo-molecular ions ([MH-123](+)) and three pairs of monoacyl containing fragment ions. All molecular species in mixed peaks were readily identified and many minor components, earlier not encountered in the samples under investigation, were identified. Acyl chain regioisomers were readily distinguished by the ratio of the [MH-RCHCO](+) ions. Molecular species differing only in the position of the double bonds in one polyunsaturated acyl chain were separated on the basis of retention times. A half quantitative estimation of the molecular species composition of complex samples was achieved by a combination of UV detection and, for mixed peaks, the areas of [MH-123](+) ions.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Dobson G,Deighton Ndoi
10.1016/s0009-3084(01)00130-xsubject
Has Abstractpub_date
2001-05-01 00:00:00pages
1-17issue
1eissn
0009-3084issn
1873-2941pii
S0009-3084(01)00130-Xjournal_volume
111pub_type
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journal_title:Chemistry and physics of lipids
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2021-01-08 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:2020-09-01 00:00:00
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journal_title:Chemistry and physics of lipids
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