Abstract:
:Accumulation of excess non-esterified free cholesterol (FC) in macrophages is a key factor in macrophage death during late stages of atheroslerosis. Raising FC content in macrophages has been shown to trigger Rac activation and actin polymerisation and to inhibit cell migration. Here, the plasma membrane distribution of the fluorescent cholesterol-mimicking sterol dehydroergosterol (DHE) was investigated in FC-loaded J774 macrophages. Wide field fluorescence and deconvolution microscopy were combined with quantitative assessment of sterol distribution in straightened plasma membrane image segments. DHE's surface distribution matched exactly large ruffles and membrane protrusions which were pronounced in FC-loaded cells. Plasma membrane blebs, however, formed in FC-loaded J774 cells had a homogenous staining along the membrane bilayer at 20 degrees C. The results show that even in FC-loaded cells with increased membrane cholesterol content, sterols do not form a separate phase in the plasma membrane.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Wüstner Ddoi
10.1016/j.chemphyslip.2008.04.009subject
Has Abstractpub_date
2008-08-01 00:00:00pages
129-36issue
2eissn
0009-3084issn
1873-2941pii
S0009-3084(08)00083-2journal_volume
154pub_type
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更新日期:1982-12-01 00:00:00
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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更新日期:2004-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:2014-02-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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更新日期:2017-01-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:1991-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2018-11-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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更新日期:1990-09-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00