Abstract：
:Most spinal cord injury research is undertaken using in vivo animal models but the extensive care associated with spinalized animals, inherent variability between animals, and complex surgeries makes alternative models especially valuable. Here we present a novel ex vivo model that enables culture of intact post-natal spinal cord segments for up to five days and the assessment of peripheral nerve grafting repair, enhanced with connexin43 antisense oligodeoxynucleotides (Cx43 AsODN), in this model. Down-regulating Cx43 expression with Cx43 AsODN in cultured spinal cord segments prevents cell death and inhibits inflammation spreading from the site of injury to neighbouring tissue, hence maintaining culture viability. Reduction in segment swelling and improvement in neuron survival were evident after Cx43 AsODN treatment. Furthermore, the combination of Cx43 AsODN with peripheral nerve graft implants into cultured spinal cords promoted axon sprouting from the spinal cord into the peripheral nerve graft. This ex vivo spinal cord segment culture model provides a valuable addition to tools currently available for spinal cord injury research.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Zhang J,O'Carroll SJ,Wu A,Nicholson LF,Green CRdoi
10.1016/j.jneumeth.2010.07.012subject
Has Abstractpub_date
2010-09-30 00:00:00pages
49-57issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(10)00386-9journal_volume
192pub_type
杂志文章abstract：BACKGROUND:Brain parcellation is important for exploiting neuroimaging data. Variability in physiology between individuals has led to the need for data-driven approaches to parcellation, with recent research focusing on simultaneously estimating and partitioning the network structure of the brain. NEW METHOD:We view d...
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