Abstract:
:We have used an in vitro system to monitor the effects of high levels of ammonia and ethanol on glial cells. Nuclei were isolated and the protein profiles examined by two-dimensional gel electrophoresis. Acute exposure of rat astrocyte cell cultures to ammonia or ethanol resulted in changes in cellular morphology and the level of some nuclear proteins. Glial fibrillary acidic protein (GFAP) levels remained constant under both treatments. Several nuclear proteins were increased specifically. Only one protein was visually detected which was unique to treatment with ammonia or ethanol. This protein (p2a) appeared only in the presence of ammonia. There were no changes in previously observed astrocyte-associated proteins (Silverman et al. Neurochem Int.12, 513-518, 1988). Two proteins appeared de novo upon either treatment with either ammonia or ethanol. These latter proteins had a molecular weight and pI profile similar to the major class of nuclear stress proteins (hsp70). However, results from immunoblot experiments clearly demonstrated that hsp70 was not induced in astroycte cultures following exposure to ammonia or ethanol.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Lipsky RH,Vemuri MC,Silverman SJdoi
10.1016/0197-0186(88)90037-xsubject
Has Abstractpub_date
1988-01-01 00:00:00pages
519-24issue
4eissn
0197-0186issn
1872-9754pii
0197-0186(88)90037-Xjournal_volume
12pub_type
杂志文章abstract::Dopamine is the main catecholamine found in the chick retina whereas norepinephrine is only found in trace amounts. We compared the effectiveness of dopamine and norepinephrine in promoting cyclic AMP accumulation in retinas at embryonic day 13 (E13) and from post-hatched chicken (P15). Dopamine (EC(50)=10microM) and ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2006.08.004
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:2014-09-01 00:00:00
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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更新日期:1997-11-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
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journal_title:Neurochemistry international
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更新日期:1985-01-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2015.08.013
更新日期:2015-11-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2003.11.007
更新日期:2004-09-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(89)90138-1
更新日期:1989-01-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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journal_title:Neurochemistry international
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journal_title:Neurochemistry international
pub_type: 杂志文章,评审
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journal_title:Neurochemistry international
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doi:10.1016/j.neuint.2007.09.009
更新日期:2008-03-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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更新日期:2002-04-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:2006-12-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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journal_title:Neurochemistry international
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
abstract::Activation of astrocytes occurs during many forms of CNS injury, but its importance for neuronal survival is poorly understood. When hippocampal cultures of neurons and astrocytes were treated from day 2-4 in vitro (DIV 2-4) with 1 microM cytosine arabinofuranoside (AraC), we observed a stellation of astrocytes, an in...
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