Abstract：
:In Russia, live vaccine strains Brucella abortus 82 and B. abortus 75/79-AV have been widely and extensively utilized for specific prophylaxis of cattle brucellosis. To differentiate these vaccine strains from each other and laboratory collection of other vaccine (n=4), reference (n=15) and field Brucella strains (n=61), the multiple-locus variable-number tandem repeat (VNTR) analyses (MLVA) were used with 12 loci containing tandem repeats from 134bp to 8bp recently described for Brucella spp. The results obtained by MLVA typing revealed that the vaccines are genetically closely related. Three sufficiently stable in vitro VNTR loci were chosen in order to discriminate, in single tube Multiplex PCR, products of the vaccine strains from the other Brucella strains. Our results demonstrated that MLVA in Multiplex PCR format is a rapid, easy, economical and efficient tool for identification of vaccine strains and will be validated in future large scale typing of Brucella isolates circulating in Russia.
journal_name
Vaccinejournal_title
Vaccineauthors
Kulakov YK,Zheludkov MM,Sclyarov ODdoi
10.1016/j.vaccine.2010.03.051subject
Has Abstractpub_date
2010-10-01 00:00:00pages
F41-5eissn
0264-410Xissn
1873-2518pii
S0264-410X(10)00451-2journal_volume
28 Suppl 5pub_type
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