Abstract:
:Cellular hypoxia can lead to cell death or adaptation and has important effects on development, physiology, and pathology. Here, we investigated the role and regulation of ceramide in hypoxia-induced apoptosis of SH-SY5Y neuroblastoma cells. Hypoxia increased the ceramide concentration; subsequently, we observed biochemical changes indicative of apoptosis, such as DNA fragmentation, nuclear staining, and poly ADP-ribose polymerase (PARP) cleavage. The hypoxic cell death was potently inhibited by a caspase inhibitor, zVAD-fmk (benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone). l-Cycloserine, a serine palmitoyltransferase (SPT) inhibitor, and fumonisin B(1) (FB(1)), a ceramide synthase inhibitor, inhibited the hypoxia-induced increase in ceramide, indicating that the increase occurred via the de novo pathway. Hypoxia increased the activity and protein levels of SPT2, suggesting that the hypoxia-induced increase in ceramide is due to the transcriptional up-regulation of SPT2. Specific siRNA of SPT2 prevented hypoxia-induced cell death and ceramide production. However, hypoxia also increased the cellular level of glucosylceramide, which was inhibited by a glucosylceramide synthase (GCS) inhibitor and specific siRNA, but not a ceramidase inhibitor. The increase in glucosylceramide was accompanied by increases in both PARP cleavage and DNA fragmentation. Together, the current results suggest that both SPT and GCS may regulate the cellular level of ceramide, and thus may be critical enzymes for deciding the fate of the cells exposed to hypoxia.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Kang MS,Ahn KH,Kim SK,Jeon HJ,Ji JE,Choi JM,Jung KM,Jung SY,Kim DKdoi
10.1016/j.cellsig.2009.11.015subject
Has Abstractpub_date
2010-04-01 00:00:00pages
610-8issue
4eissn
0898-6568issn
1873-3913pii
S0898-6568(09)00350-7journal_volume
22pub_type
杂志文章abstract::Mice lacking the gene for suppressor of cytokine signaling 1 (SOCS1) show defective homeostasis of T lymphocytes due to accumulation of CD8(+) T cells, resulting at least partly from dysregulated IL-15 signaling. IL-15 alone does not stimulate proliferation of naïve CD8 T cells, but can synergize with IL-21 to induce ...
journal_title:Cellular signalling
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journal_title:Cellular signalling
pub_type: 杂志文章,评审
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更新日期:2015-09-01 00:00:00
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journal_title:Cellular signalling
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journal_title:Cellular signalling
pub_type: 杂志文章
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更新日期:2014-02-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2006.12.007
更新日期:2007-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0898-6568(02)00055-4
更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/j.cellsig.2014.11.010
更新日期:2015-02-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2007.04.007
更新日期:2007-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.cellsig.2008.10.021
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2014.08.005
更新日期:2014-12-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2020.109549
更新日期:2020-05-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/0898-6568(95)02017-9
更新日期:1996-01-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
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journal_title:Cellular signalling
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doi:10.1016/j.cellsig.2011.03.016
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journal_title:Cellular signalling
pub_type: 杂志文章,评审
doi:10.1016/j.cellsig.2012.11.019
更新日期:2013-03-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2005.05.004
更新日期:2006-03-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章,评审
doi:10.1016/j.cellsig.2020.109577
更新日期:2020-06-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章,评审
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更新日期:2003-11-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章,评审
doi:10.1016/j.cellsig.2004.05.004
更新日期:2004-12-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2004.03.014
更新日期:2004-10-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2008.05.003
更新日期:2008-09-01 00:00:00
abstract::The TAK1 plays a pivotal role in the innate immune response of Drosophila by controlling the activation of JNK and NF-kappaB. Activation of TAK1 in mammals is mediated by two TAK1-binding proteins, TAB1 and TAB2, but the role of the TAB proteins in the immune response of Drosophila has not yet been established. Here, ...
journal_title:Cellular signalling
pub_type: 杂志文章
doi:10.1016/j.cellsig.2005.08.020
更新日期:2006-07-01 00:00:00
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journal_title:Cellular signalling
pub_type: 杂志文章
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