Abstract：
:Regulator of G Protein Signalling (RGS) proteins impede heterotrimeric G protein signalling. RGS2 decreases cAMP production and appears to interact with both adenylyl cyclase (AC) and its stimulatory G protein Gs. We showed previously that Green Fluorescent Protein-tagged RGS2 (GFP-RGS2) localizes to the nucleus in HEK 293 cells and is recruited to the plasma membrane when co-expressed with Gsalpha, or the Gs-coupled beta2-adrenergic receptor (beta2AR). Here, using confocal microscopy we show that co-expression of various AC isoforms (ACI, ACII, ACV, ACVI) also leads to GFP-RGS2 recruitment to the plasma membrane. Bioluminescence Resonance Energy Transfer (BRET) was also used to examine physical interactions between RGS2 and components of the Gs-signalling pathway. A BRET signal was detected between fusion constructs of RGS2-Renilla luciferase (energy donor) and Gsalpha-GFP (energy acceptor) co-expressed in HEK 293 cells. BRET was also observed between GFP-RGS2 and ACII or ACVI fused to Renilla luciferase. Additionally, RGS2 was found to interact with the beta2AR. Purified RGS2 selectively bound to the third intracellular loop of the beta2AR in GST pulldown assays, and a BRET signal was observed between GFP-RGS2 and beta2AR fused to Renilla luciferase when these two proteins were co-expressed together with either ACIV or ACVI. This interaction was below the limit of detection in the absence of co-expressed AC, suggesting that the effector enzyme stabilized or promoted binding between the receptor and the RGS protein inside the cell. Taken together, these results suggest the possibility that RGS2 might bind to a receptor-G protein-effector signalling complex to regulate Gs-dependent cAMP production.
journal_name
Cell Signaljournal_title
Cellular signallingauthors
Roy AA,Baragli A,Bernstein LS,Hepler JR,Hébert TE,Chidiac Pdoi
10.1016/j.cellsig.2005.05.004subject
Has Abstractpub_date
2006-03-01 00:00:00pages
336-48issue
3eissn
0898-6568issn
1873-3913pii
S0898-6568(05)00110-5journal_volume
18pub_type
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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更新日期：2012-10-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2014.12.014
更新日期：2015-04-01 00:00:00
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2011.04.008
更新日期：2011-09-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章,评审
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更新日期：2011-01-01 00:00:00
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2011.11.005
更新日期：2012-03-01 00:00:00
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journal_title：Cellular signalling
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journal_title：Cellular signalling
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更新日期：2014-12-01 00:00:00
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journal_title：Cellular signalling
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doi：10.1016/j.cellsig.2007.08.013
更新日期：2007-12-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章,评审
doi：10.1016/j.cellsig.2009.02.012
更新日期：2009-08-01 00:00:00
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journal_title：Cellular signalling
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更新日期：2015-09-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2007.02.010
更新日期：2007-08-01 00:00:00
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journal_title：Cellular signalling
pub_type： 杂志文章
doi：10.1016/j.cellsig.2004.12.003
更新日期：2005-09-01 00:00:00
abstract：:Macrophages play many different roles in tissue inflammation and immunity, and the plasticity of macrophage polarization is closely associated with acute inflammatory responses. O-GlcNAcylation is an important type of post-translational modification, which subtly modulates inflammation responses. Transforming growth f...
journal_title：Cellular signalling
pub_type： 杂志文章,收录出版
doi：10.1016/j.cellsig.2016.08.008
更新日期：2016-11-01 00:00:00
