当前位置： SCI文献检索 > VACCINE期刊下所有文献 > Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase.

Measurement of neutralizing antibody responses against H5N1 clades in immunized mice and ferrets using pseudotypes expressing influenza hemagglutinin and neuraminidase.

Abstract：

:Neutralizing antibody is associated with the prevention and clearance of influenza virus infection. Microneutralization (MN) and hemagglutination inhibition (HI) assays are currently used to evaluate neutralizing antibody responses against human and avian influenza viruses, including H5N1. The MN assay is somewhat labor intensive, while HI is a surrogate for neutralization. Moreover, use of replication competent viruses in these assays requires biosafety level 3 (BSL-3) containment. Therefore, a neutralization assay that does not require BSL-3 facilities would be advantageous. Toward this goal, we generated a panel of pseudotypes expressing influenza hemagglutinin (HA) and neuraminidase (NA) and developed a pseudotype-based neutralization (PN) assay. Here we demonstrate that HA/NA pseudotypes mimic release and entry of influenza virus and that the PN assay exhibits good specificity and reveals quantitative difference in neutralizing antibody titers against different H5N1 clades and subclades. Using immune ferret sera, we demonstrated excellent correlation between the PN, MN, and HI assays. Thus, we conclude that the PN assay is a sensitive and quantifiable method to measure neutralizing antibodies against diverse clades and subclades of H5N1 influenza virus.

journal_name

Vaccine

journal_title

Vaccine

authors

Tsai C,Caillet C,Hu H,Zhou F,Ding H,Zhang G,Zhou B,Wang S,Lu S,Buchy P,Deubel V,Vogel FR,Zhou P

doi

10.1016/j.vaccine.2009.08.056

subject

Has Abstract

pub_date

2009-11-12 00:00:00

pages

6777-90

issue

48

eissn

0264-410X

issn

1873-2518

pii

S0264-410X(09)01239-0

journal_volume

27

pub_type

杂志文章

相关文献

VACCINE文献大全
  • Structural perturbation of diphtheria toxoid upon adsorption to aluminium hydroxide adjuvant.

    abstract：:Aluminium-containing adjuvants are often used to enhance the potency of vaccines. In the present work we studied whether adsorption of diphtheria toxoid to colloidal aluminium hydroxide induces conformational changes of the antigen. Diphtheria toxoid has a high affinity for the aluminium hydroxide particles based on a...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.09.020

    authors： Régnier M,Metz B,Tilstra W,Hendriksen C,Jiskoot W,Norde W,Kersten G

    更新日期：2012-11-06 00:00:00

  • The web and public confidence in MMR vaccination in Italy.

    abstract：:Measles, mumps and rubella (MMR) vaccination coverage in Italy has been decreasing starting from 2012 and, at the present, none of the Italian regions has achieved the goal of 95% coverage target. A decision of the Court of Justice of Rimini in March 2012 that awarded vaccine-injury compensation for a case of autism h...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.07.029

    authors： Aquino F,Donzelli G,De Franco E,Privitera G,Lopalco PL,Carducci A

    更新日期：2017-08-16 00:00:00

  • Improved haemagglutinin antigen content in H5N1 candidate vaccine viruses with chimeric haemagglutinin molecules.

    abstract：:The candidate vaccine virus NIBRG-14 was derived by reverse genetics and comprises the haemagglutinin (HA) and neuraminidase (NA) genes derived from the clade 1 virus A/Viet Nam/1194/2004 on an A/Puerto Rico/8/34 (PR8) backbone. The HA gene was modified to remove the multibasic cleavage site motif associated with high...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2010.09.006

    authors： Harvey R,Nicolson C,Johnson RE,Guilfoyle KA,Major DL,Robertson JS,Engelhardt OG

    更新日期：2010-11-23 00:00:00

  • Tetanus vaccination is associated with differential DNA-methylation: Reduces the risk of asthma in adolescence.

    abstract：BACKGROUND:Vaccinations have been suggested to be associated with increased risk of allergic diseases. Tetanus vaccination is one of the most frequently administered vaccines as a part of wound management and was also found to be associated with increased serum IgE levels. We hypothesized that the vaccination modifies ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2016.10.068

    authors： Janjanam VD,Mukherjee N,Lockett GA,Rezwan FI,Kurukulaaratchy R,Mitchell F,Zhang H,Arshad H,Holloway JW,Karmaus W

    更新日期：2016-12-12 00:00:00

  • Mumps resurgences in the United States: A historical perspective on unexpected elements.

    abstract：:In 2006 the United States experienced the largest nationwide mumps epidemic in 20 years, primarily affecting college dormitory residents. Unexpected elements of the outbreak included very abrupt time course (75% of cases occurred within 90 days), geographic focality (85% of cases occurred in eight rural Midwestern sta...

    journal_title：Vaccine

    pub_type： 历史文章,杂志文章,评审

    doi：10.1016/j.vaccine.2009.06.109

    authors： Barskey AE,Glasser JW,LeBaron CW

    更新日期：2009-10-19 00:00:00

  • Effect of zinc and vitamin A supplementation on antibody responses to a pneumococcal conjugate vaccine in HIV-positive injection drug users: a randomized trial.

    abstract：:HIV-infected individuals have impaired immune responses to vaccines and high rates of pneumococcal disease. The effect of vitamin A and zinc supplementation on the immunogenicity of a 7-valent pneumococcal CRM-197 conjugate vaccine (PC-7) was evaluated in 118 HIV+ injection drug users. Subjects were randomized to oral...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2005.09.047

    authors： Deloria-Knoll M,Steinhoff M,Semba RD,Nelson K,Vlahov D,Meinert CL

    更新日期：2006-03-06 00:00:00

  • Efficacy, safety and immunogenicity of RIX4414 in Japanese infants during the first two years of life.

    abstract：:A phase III, randomized, double-blind study evaluated the efficacy, reactogenicity, safety and immunogenicity of a human rotavirus vaccine, RIX4414 in Japanese infants aged 6-14 weeks when administered as two doses (0, 1-month schedule). Efficacy against any and severe rotavirus gastroenteritis leading to medical inte...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.vaccine.2011.05.017

    authors： Kawamura N,Tokoeda Y,Oshima M,Okahata H,Tsutsumi H,Van Doorn LJ,Muto H,Smolenov I,Suryakiran PV,Han HH

    更新日期：2011-08-26 00:00:00

  • Formulation of botulinum neurotoxin heavy chain fragments for vaccine development: mechanisms of adsorption to an aluminum-containing adjuvant.

    abstract：:Heavy chain fragments of botulinum neurotoxin serotypes A and B are being developed as a bivalent vaccine for botulism. To potentiate the immune response, an aluminum containing adjuvant will be formulated with the two antigens. The adsorption mechanisms of each antigen to aluminum phosphate and aluminum hydroxide adj...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2005.03.028

    authors： DePaz RA,Henderson I,Advant SJ

    更新日期：2005-07-01 00:00:00

  • Economics of polio vaccination in the post-eradication era: should OPV-using countries adopt IPV?

    abstract：:The continued use of oral polio vaccine (OPV) poses a threat to polio virus eradication. Stopping all polio vaccination in the post-certification era is no longer considered to be a practical option. Policy makers agree that OPV use must stop immediately after certification. Therefore, the pragmatic alternative is for...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.02.008

    authors： Khan MM

    更新日期：2008-04-07 00:00:00

  • Observational study of vaccine effectiveness 20 years after the introduction of universal hepatitis B vaccination in Tunisia.

    abstract：OBJECTIVES:The objectives of this study were to estimate the national prevalence of hepatitis B infection in Tunisia using data from a nationwide survey, to compare results with those obtained in 1996 survey and to evaluate the impact of vaccination twenty years after its introduction. METHODS:A National household-bas...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2018.08.038

    authors： Ben Hadj M,Bouguerra H,Saffar F,Chelly S,Hechaichi A,Talmoudi K,Bahrini A,Chouki T,Hazgui O,Hannachi N,Letaief H,Bellali H,Bahri O,Ben-Alaya-Bouafif N

    更新日期：2018-09-18 00:00:00

  • The role of anti-NHba antibody in bactericidal activity elicited by the meningococcal serogroup B vaccine, MenB-4C.

    abstract：BACKGROUND:MenB-4C (Bexsero®) is a multicomponent serogroup B meningococcal vaccine. For vaccine licensure, efficacy was inferred from serum bactericidal antibody (SBA) against three antigen-specific indicator strains. The bactericidal role of antibody to the fourth vaccine antigen, Neisserial Heparin binding antigen (...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.06.020

    authors： Partridge E,Lujan E,Giuntini S,Vu DM,Granoff DM

    更新日期：2017-07-24 00:00:00

  • DNA vaccination with the serine rich Entamoeba histolytica protein (SREHP) prevents amebic liver abscess in rodent models of disease.

    abstract：:Amebiasis remains one of the leading parasitic causes of death worldwide. A vaccine that prevented amebic liver abscess would significantly reduce mortality from this disease. To test the feasibility of a DNA vaccine to prevent amebic liver abscess, we immunized both mice and gerbils with plasmid DNA encoding the seri...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/s0264-410x(99)00343-6

    authors： Zhang T,Stanley SL Jr

    更新日期：1999-12-10 00:00:00

  • Flagellin-HA vaccines protect ferrets and mice against H5N1 highly pathogenic avian influenza virus (HPAIV) infections.

    abstract：:In order to meet the global demand for rapid production of pandemic influenza vaccines, we have developed a recombinant fusion vaccine platform in which the globular head of hemagglutinin (HA) antigen is genetically fused to bacterial flagellin (a TLR5 ligand). These flagellin-HA fusion vaccine candidates elicit highl...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.09.013

    authors： Liu G,Song L,Reiserova L,Trivedi U,Li H,Liu X,Noah D,Hou F,Weaver B,Tussey L

    更新日期：2012-11-06 00:00:00

  • The outer membrane protein, LamB (maltoporin), is a versatile vaccine candidate among the Vibrio species.

    abstract：:Maltoporin (LamB) is a family of outer membrane proteins. There has been no report of immunological characteristics of LamB in the Vibrio species so far. In this study, lamB genes from eight Vibrio strains were cloned and sequenced. The bioinformatics analysis indicated that sequence similarities of LamB proteins were...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2013.12.035

    authors： Lun J,Xia C,Yuan C,Zhang Y,Zhong M,Huang T,Hu Z

    更新日期：2014-02-07 00:00:00

  • Novel antibody-independent receptor-binding SPR-based assay for rapid measurement of influenza vaccine potency.

    abstract：:A WHO workshop organized following the 2009 H1N1 pandemic recommended development of alternative influenza vaccine potency assays as high priority that could expedite the release of vaccine lots in the face of future influenza pandemics. We have developed an antibody independent, simple, high throughput receptor-bindi...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2014.02.049

    authors： Khurana S,King LR,Manischewitz J,Coyle EM,Golding H

    更新日期：2014-04-17 00:00:00

  • Leptospiral immunoglobulin-like proteins elicit protective immunity.

    abstract：:The identification of new vaccine candidates for leptospirosis is of great interest. This paper describes the identification of two homologous immunogenic proteins, LigA-m and LigB-m, of Leptospira interrogans serovar manilae strain UP-MMC-NIID, which show strong similarity with LigA found recently in L. interrogans s...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2003.10.007

    authors： Koizumi N,Watanabe H

    更新日期：2004-03-29 00:00:00

  • Accelerated and long term stability study of Pfs25-EPA conjugates adjuvanted with Alhydrogel®.

    abstract：:Pfs25, a Plasmodium falciparum surface protein expressed during zygote and ookinete stages in infected mosquitoes, is a lead transmission-blocking vaccine candidate against falciparum malaria. To enhance immunogenicity, recombinant Pfs25 was chemically conjugated to recombinant nontoxic Pseudomonas aeruginosa ExoProte...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2017.04.067

    authors： Zhu D,Wu Y,McClellan H,Dai W,Rausch K,Jones D,Aebig J,Barnafo E,Butler B,Lambert L,Narum DL,Duffy PE

    更新日期：2017-05-31 00:00:00

  • Eimeria species parasites as novel vaccine delivery vectors: anti-Campylobacter jejuni protective immunity induced by Eimeria tenella-delivered CjaA.

    abstract：:Vaccination of poultry against coccidiosis caused by the Eimeria species is almost entirely based upon varied formulations of live parasites. The recent development of a series of protocols that support genetic complementation by transfection in Eimeria now provides an opportunity to utilise live anticoccidial vaccine...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2012.02.002

    authors： Clark JD,Oakes RD,Redhead K,Crouch CF,Francis MJ,Tomley FM,Blake DP

    更新日期：2012-03-30 00:00:00

  • Immunization with an engineered mutant trans-sialidase highly protects mice from experimental Trypanosoma cruzi infection: a vaccine candidate.

    abstract：:Chagas' disease is a major tropical disease for which a cure for chronic phase does not exist yet. Trypanosoma cruzi trans-sialidase (TS) seems to be involved in relevant processes such as infectivity, host survival and, very importantly, disease pathogenesis. In this study, we show that mice vaccinated with an engine...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.02.060

    authors： Fontanella GH,De Vusser K,Laroy W,Daurelio L,Nocito AL,Revelli S,Contreras R

    更新日期：2008-05-02 00:00:00

  • Development of a membrane adsorber based capture step for the purification of yellow fever virus.

    abstract：:Yellow fever (YF) is an endemic disease in some tropical areas of South America and Africa that presents lethality rate between 20 and 50%. There is no specific treatment and to control this disease a highly effective live-attenuated egg based vaccine is widely used for travelers and residents of areas where YF is end...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2014.02.036

    authors： Pato TP,Souza MC,Silva AN,Pereira RC,Silva MV,Caride E,Gaspar LP,Freire MS,Castilho LR

    更新日期：2014-05-19 00:00:00

  • Needle-free jet injector intradermal delivery of fractional dose inactivated poliovirus vaccine: Association between injection quality and immunogenicity.

    abstract：INTRODUCTION:The World Health Organization recommends that as part of the polio end-game strategy a dose of inactivated poliovirus vaccine (IPV) be introduced by the end of 2015 in all countries currently using only oral poliovirus vaccine (OPV). Administration of fractional dose (1/5 of full dose) IPV (fIPV) by intrad...

    journal_title：Vaccine

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.vaccine.2015.06.071

    authors： Resik S,Tejeda A,Mach O,Sein C,Molodecky N,Jarrahian C,Saganic L,Zehrung D,Fonseca M,Diaz M,Alemany N,Garcia G,Hung LH,Martinez Y,Sutter RW

    更新日期：2015-10-26 00:00:00

  • The path towards effective antivirals against rabies.

    abstract：:Rabies virus remains an important burden of disease claiming an estimated 60,000 lives each year, mainly children, and having a huge economical and societal cost. Post-exposure prophylaxis (PEP) is highly effective, however in patients that present with neurological symptoms the case-fatality ratio is extremely high (...

    journal_title：Vaccine

    pub_type： 杂志文章,评审

    doi：10.1016/j.vaccine.2017.12.051

    authors： Jochmans D,Neyts J

    更新日期：2019-08-02 00:00:00

  • Predictors of interest in HPV vaccination: A study of British adolescents.

    abstract：:Human papillomavirus (HPV) vaccination is now offered to adolescent girls in the UK. Adolescents over 16 years old are likely to make their own decision about the vaccination. The purpose of this cross-sectional study was to assess acceptability of HPV vaccination among female adolescents (16 -- 19 years) and investig...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2009.02.057

    authors： Marlow LA,Waller J,Evans RE,Wardle J

    更新日期：2009-04-21 00:00:00

  • A novel baculovirus vector shows efficient gene delivery of modified porcine reproductive and respiratory syndrome virus antigens and elicits specific immune response.

    abstract：:Porcine reproductive and respiratory syndrome (PRRS) is an economically devastating epizootic of porcine species. Current vaccines are inadequate to control the disease burden and outbreaks in the field. We report a novel baculovirus vaccine vector with White spot syndrome virus immediate early 1 shuttle promoter, wit...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2013.08.101

    authors： Karuppannan AK,Qiang J,Chang CC,Kwang J

    更新日期：2013-11-04 00:00:00

  • Immunosuppressive drugs impairs antibody response of the polysaccharide and conjugated pneumococcal vaccines in patients with Crohn's disease.

    abstract：BACKGROUND:Patients with Crohn's disease (CD) have a higher risk of infectious diseases including pneumococcal infections, and the risk increases with immunotherapy. The primary endpoint of this study was to investigate the specific antibody response to two pneumococcal vaccines in CD patients with and without immunosu...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1016/j.vaccine.2015.08.011

    authors： Kantsø B,Halkjær SI,Thomsen OØ,Belard E,Gottschalck IB,Jørgensen CS,Krogfelt KA,Slotved HC,Ingels H,Petersen AM

    更新日期：2015-10-05 00:00:00

  • Sero-epidemiology of measles and mumps in Korea: impact of the catch-up campaign on measles immunity.

    abstract：:A catch-up campaign targeting children aged 8-16 years using measles-rubella (MR) vaccine was conducted during 2001 in Korea. To evaluate the impact of the campaign and assess mumps immunity, human IgG antibodies were detected using ELISA for measles (5826 samples) and mumps (5890 samples) in a national sample of oppo...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.vaccine.2004.07.030

    authors： Kim SS,Han HW,Go U,Chung HW

    更新日期：2004-12-02 00:00:00

  • ArtinM, a D-mannose-binding lectin from Artocarpus integrifolia, plays a potent adjuvant and immunostimulatory role in immunization against Neospora caninum.

    abstract：:ArtinM and Jacalin (JAC) are lectins from the jackfruit (Artocarpus integrifolia) that have important role in modulation of immune responses to pathogens. Neospora caninum is an Apicomplexa parasite that causes neuromuscular disease in dogs and reproductive disorders in cattle, with economic impact on the livestock in...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2011.09.136

    authors： Cardoso MR,Mota CM,Ribeiro DP,Santiago FM,Carvalho JV,Araujo EC,Silva NM,Mineo TW,Roque-Barreira MC,Mineo JR,Silva DA

    更新日期：2011-11-15 00:00:00

  • Five winters of pneumococcal serotype replacement in UK carriage following PCV introduction.

    abstract：:The seven-valent pneumococcal conjugate vaccine (PCV7) was added to the UK national immunisation programme in September 2006. PCV13 replaced PCV7 in April 2010. As carriage precedes disease cases this study collected carried pneumococci from children each winter from 2006/7 to 2010/11 over PCV introduction. Convention...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2015.03.012

    authors： Gladstone RA,Jefferies JM,Tocheva AS,Beard KR,Garley D,Chong WW,Bentley SD,Faust SN,Clarke SC

    更新日期：2015-04-21 00:00:00

  • Immunogenicity and safety of the inactivated Japanese encephalitis vaccine IXIARO® in elderly subjects: Open-label, uncontrolled, multi-center, phase 4 study.

    abstract：BACKGROUND:IXIARO® is a Vero cell-derived, inactivated Japanese encephalitis (JE) vaccine licensed mainly in western countries for children and adults traveling to JE endemic areas. Limited immunogenicity and safety data in elderly travelers have been available. OBJECTIVES:To evaluate safety and immunogenicity of IXIA...

    journal_title：Vaccine

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.vaccine.2016.07.029

    authors： Cramer JP,Dubischar K,Eder S,Burchard GD,Jelinek T,Jilma B,Kollaritsch H,Reisinger E,Westritschnig K

    更新日期：2016-08-31 00:00:00

  • A nasal vaccine comprising B-subunit derivative of Shiga toxin 2 for cross-protection against Shiga toxin types 1 and 2.

    abstract：:Enterohemorrhagic Escherichia coli (EHEC) produces Stx1 and Stx2 causing severe diseases. Their B-subunits (StxBs) exhibit low immunogenicity and the anti-StxB antibodies neutralizing both Stxs has not been prepared yet. By intranasal vaccination with His-tagged-StxB (Stx1B-His or Stx2B-His) plus a mutant heat-labile ...

    journal_title：Vaccine

    pub_type： 杂志文章

    doi：10.1016/j.vaccine.2008.02.034

    authors： Tsuji T,Shimizu T,Sasaki K,Tsukamoto K,Arimitsu H,Ochi S,Shimizu T,Taniguchi K,Noda M,Neri P,Mori H

    更新日期：2008-04-16 00:00:00