Abstract:
:A WHO workshop organized following the 2009 H1N1 pandemic recommended development of alternative influenza vaccine potency assays as high priority that could expedite the release of vaccine lots in the face of future influenza pandemics. We have developed an antibody independent, simple, high throughput receptor-binding SPR-based potency assay, which does not require any reference antisera and could be used for rapid HA quantitation and vaccine release in pandemic scenarios. The assay utilizes synthetic glycans with sialic acid (SA) of either α-2,6 or α-2,3 linkage to galactose. Only functionally active forms of HA (trimers and oligomers) recognize the SA-glycans and are quantified in this receptor-binding SPR assay. The SA-glycan SPR assay demonstrated broad dynamic range for quantitation of HA content in influenza vaccines from different manufacturers for both seasonal (A/H1N1, A/H3N2, B lineages) and pandemic influenza (A/H5N1, A/H7N9) strains with high reproducibility and low variability across multiple assays. In addition, the SA-glycan SPR assay is indicative of active HA stability, and can accurately quantify HA content in alum and oil-in-water adjuvanted influenza vaccines. Importantly, there was a good agreement between HA content determined by the SPR-based potency assay and the traditional SRID assay.
journal_name
Vaccinejournal_title
Vaccineauthors
Khurana S,King LR,Manischewitz J,Coyle EM,Golding Hdoi
10.1016/j.vaccine.2014.02.049subject
Has Abstractpub_date
2014-04-17 00:00:00pages
2188-97issue
19eissn
0264-410Xissn
1873-2518pii
S0264-410X(14)00234-5journal_volume
32pub_type
杂志文章相关文献
VACCINE文献大全abstract::Plasmid DNA is a promising vaccine platform that has been shown to be safe and able to be administered repeatedly without vector interference. Enhancing the potency of DNA vaccination through co-delivery of molecular adjuvants is one strategy currently under investigation. Here we describe the use of the novel chemoki...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.10.095
更新日期:2010-02-23 00:00:00
abstract::The outer capsid protein VP4 is an important target for the development of a recombinant rotavirus vaccine because it mediates the attachment and penetration of rotavirus. Due to the poor solubility of full-length VP4, VP8 was explored as candidate rotavirus vaccines in the past years. In previous studies, it has been...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2018.03.011
更新日期:2018-04-12 00:00:00
abstract::Sanaria Inc. has developed methods to manufacture, purify and cryopreserve aseptic Plasmodium falciparum (Pf) sporozoites (SPZ), and is using this platform technology to develop an injectable PfSPZ-based vaccine that provides high-grade, durable protection against infection with Pf malaria. Several candidate vaccines ...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2015.09.096
更新日期:2015-12-22 00:00:00
abstract::The prevalence of seropositive individuals that makes costs of vaccinating all individuals equal to that for screening and vaccination of susceptible individuals is defined as the critical prevalence of antibodies (p*). Screening and vaccination is more efficient when the prevalence of seropositive individuals (p) in ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2004.03.048
更新日期:2004-09-28 00:00:00
abstract:BACKGROUND:Protein-polysaccharide conjugate vaccines such as Haemophilus influenzae type b (Hib), meningococcal, and pneumococcal vaccine, induce immunological memory and longer lasting protection than plain polysaccharide vaccines. The most common proteins used as carriers are tetanus toxoid (TT) and cross reacting ma...
journal_title:Vaccine
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.vaccine.2016.06.038
更新日期:2016-07-25 00:00:00
abstract::Hepatitis B infection and its sequelae remain major public health problems internationally despite the existence of sensitive tests to screen blood and blood products for hepatitis B surface antigen (HBsAg) and immunogenic vaccines. Since the human hepatitis B virus has no known animal reservoir, a systematic vaccinat...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(90)90233-c
更新日期:1990-03-01 00:00:00
abstract::Pneumococcal conjugate vaccines (PCV) may reduce nasopharyngeal carriage (NPC) of Streptococcus pneumoniae vaccine strains (VT), but serotype replacement with non-vaccine strains (NVT) has been reported. Bacterial NPC after PHiD-CV vaccination was assessed in the second year of life. Open descriptive study of NPC repo...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.12.086
更新日期:2011-02-24 00:00:00
abstract:BACKGROUND:Pregnant women are at risk of severe influenza disease and are a priority group for influenza vaccination programs. Nicaragua expanded recommendations to include influenza vaccination to all pregnant women in the municipality of Managua in 2013. METHODS:We carried out a survey among 1,807 pregnant women who...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.12.065
更新日期:2016-02-17 00:00:00
abstract::Human chorionic gonadotropin (hCG) is currently under investigation as an antigenic target in both anti-cancer and anti-fertility vaccines. Formulations studied to date show promise in clinical trials for both applications yet are expensive to produce and require frequented administration in order to maintain an effec...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(96)00046-1
更新日期:1996-11-01 00:00:00
abstract::A formalin-inactivated red sea bream iridovirus (RSIV) vaccine was prepared using the culture supernatant of a persistently infected Pagrus major fin cell line (PI-PMF) with IVS-1 strain (RSIV subtype II Meglaocytivirus). Rock bream (Oplegnathus fasciatus) were injected with a high-dose, ultracentrifuged megalocytivir...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.11.001
更新日期:2020-12-03 00:00:00
abstract::A growing number of people use the Internet to obtain health information, including information about vaccines. Websites that allow and promote interaction among users are an increasingly popular source of health information. Users of such so-called Web 2.0 applications (e.g. social media), while still in the minority...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.02.025
更新日期:2012-05-28 00:00:00
abstract::The replication-defective herpes simplex virus 2 (HSV-2) dl5-29 mutant virus strain with deletions in the U(L)5 and U(L)29 genes has been shown to protect mice and guinea pigs against challenge with wild-type (wt) HSV-2 and to protect against ocular disease caused by HSV-1 infection. The dl5-29 strain is currently bei...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.01.030
更新日期:2010-03-24 00:00:00
abstract::A new 13-valent conjugated polysaccharide vaccine (PCV13) against Streptococcus pneumoniae infections, which replaced the 7-valent vaccine (PCV7) in the regional immunization programmes for newborns and children who started but not completed the 3 doses schedule of PCV7, is available in Italy since 2010. The opportuni...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.10.013
更新日期:2011-11-28 00:00:00
abstract::Protection against Schistosoma mansoni infection in C57BL/6 female mice was evaluated by two DNA vaccination strategies. Mice were either vaccinated by intramuscular injection with pcDNAI/Amp constructs encoding either Cu/Zn cytosolic superoxide dismutase (CT-SOD), signal peptide-containing SOD (SP-SOD), glutathione p...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00535-8
更新日期:2003-12-08 00:00:00
abstract::A major impediment to the development of peptide vaccines has been the inability accurately to mimic conformationally constrained epitopes on the envelope proteins of pathogens. This limitation is further compounded by the fact that several viral envelope proteins exist either as covalently or non-covalently associate...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(92)90152-a
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND:The primary prevention measure for influenza infection has been the use of influenza vaccines. However, even when the vaccine and circulating strains are well-matched, some healthy children do not respond to the vaccine, likely due to a genetic basis for immune hyporesponsiveness. The primary objective of th...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.12.026
更新日期:2013-02-04 00:00:00
abstract::In Portugal, the 7-valent pneumococcal conjugate vaccine (PCV7) was not introduced in the national immunization plan but was commercially available between 2001 and 2010. We studied serotype distribution and antibiotic susceptibility of Streptococcus pneumoniae carried by children in 2009 and 2010. Vaccination with PC...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2016.02.045
更新日期:2016-03-29 00:00:00
abstract::Codon-pair bias de-optimization (CPBD) of viruses involves re-writing viral genes using statistically underrepresented codon pairs, without any changes to the amino acid sequence or codon usage. Previously, this technology has been used to attenuate the influenza A/Puerto Rico/8/34 (H1N1) virus. The de-optimized virus...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.11.054
更新日期:2016-01-20 00:00:00
abstract::Purified tetanus toxoid, a high-molecular-weight protein, was entrapped within poly(L-lactic acid) (PLA) and poly(D,L-lactic/glycolic acid) (PLGA) microspheres prepared by either a solvent extraction or a solvent evaporation method carried out in a multiple emulsion system (water-in-oil-in-water). The physical integri...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)90092-2
更新日期:1994-03-01 00:00:00
abstract::One approach to develop a malaria blood-stage vaccine is to target proteins that play critical roles in the erythrocyte invasion of merozoites. The merozoite surface proteins (MSPs) and the erythrocyte-binding antigens (EBAs) are considered promising vaccine candidates, for they are known to play important roles in er...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2012.01.010
更新日期:2012-03-02 00:00:00
abstract::Sera were taken over a 5 year period from Gambian children vaccinated in 1983, when aged 1-4 years, with A + C meningococcal capsular polysaccharide, ELISA tests were devised to determine the concentrations of immunoglobulin A, G and M reacting with A polysaccharide and of IgG reacting with Opc protein, IgA1 protease ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(96)00138-7
更新日期:1997-02-01 00:00:00
abstract::Long-term protection of rabbits that had been vaccinated with two doses of a recombinant protective antigen (rPA) vaccine was examined against an aerosol spore challenge with the Ames isolate of Bacillus anthracis at 6 and 12 months. At 6 months after the primary injection, survival was 74.1% (20/27) with quantitative...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.12.028
更新日期:2006-03-24 00:00:00
abstract::The calculation of p(epitope) values, a sequence-based measure of antigenic distance between strains, was developed for human influenza. The potential to apply the p(epitope) value to equine influenza vaccine strain selection was assessed. There was a negative correlation between p(epitope) value and vaccine efficacy ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2013.06.070
更新日期:2013-12-09 00:00:00
abstract::An experimental infection with classical swine fever (CSF) virus in E2 sub-unit marker vaccine vaccinated gilts was conducted in order to evaluate the effect of vaccination on virus transmission and course of the disease. Therefore, clinical signs as well as horizontal and vertical virus transmission were monitored in...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(01)00320-6
更新日期:2001-10-12 00:00:00
abstract::Although concerns about safety of influenza vaccination during pregnancy have been raised in the past, vaccination of pregnant women was recommended in many countries during the 2009 A/H1N1 pandemic influenza. A retrospective cohort study was conducted to evaluate the risk of adverse maternal, fetal and neonatal outco...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2015.03.041
更新日期:2015-05-05 00:00:00
abstract::Nucleic acid vaccination has many potential advantages over traditional methods, but suffers from the fact that DNA vaccines tend to be relatively poorly immunogenic. Attempts to enhance DNA vaccine immunogenicity have included the addition of cytokine-encoding plasmids into the formulation, as well as the use of hete...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.01.160
更新日期:2005-06-10 00:00:00
abstract:BACKGROUND:Concern arose in 2010 that reactogenicity, particularly febrile seizures, to influenza A/H1N1-containing 2010-2011 trivalent seasonal inactivated influenza vaccine (TIV) could occur in young children who had been previously immunized and/or infected with the pandemic strain. We conducted a pre-season study o...
journal_title:Vaccine
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1016/j.vaccine.2012.03.046
更新日期:2012-05-14 00:00:00
abstract:OBJECTIVE:We investigated whether and to what extent the uptake of the Human Papillomavirus (HPV) vaccine by girls aged 12-18 was related to the cervical cancer screening history of age-appropriate older female household members (assumed to be their mothers) in Flanders (Belgium). METHODS:We studied administrative rec...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.08.039
更新日期:2011-10-26 00:00:00
abstract:BACKGROUND:We evaluated the safety and immunogenicity of liquid and lyophilized formulations of an investigational trivalent group B streptococcus (GBS) vaccine in non-pregnant women and assessed the formulations' equivalence in terms of serotype-specific immune response. METHODS:This phase II, randomized, comparative...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.02.085
更新日期:2020-04-03 00:00:00
abstract::Meningococcal disease is a serious public health threat given the seriousness of the illness, its disabling sequelae and its potential for epidemic spread. The disease is a concern during mass gatherings which provide conditions that facilitate transmission of infectious agents including Neisseria meningitidis. Implem...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2018.06.030
更新日期:2018-07-25 00:00:00