Abstract:
:Plasmid DNA is a promising vaccine platform that has been shown to be safe and able to be administered repeatedly without vector interference. Enhancing the potency of DNA vaccination through co-delivery of molecular adjuvants is one strategy currently under investigation. Here we describe the use of the novel chemokine adjuvant CCL27/CTACK to enhance immune responses to an HIV-1 or SIV antigen in mice and rhesus macaques. CCL27 has been shown to play a role in inflammatory responses through chemotaxis of CCR10+ cells, and we hypothesized that CCL27 may modulate adaptive immune responses. Immunizations in mice with HIV-1gag/CCL27 enhanced immune responses both at peripheral and, surprisingly, at mucosal sites. To confirm these findings in a large-animal model, we created optimized CCL27 and SIV antigenic plasmid constructs for rhesus macaques. 10 macaques (n=5/group) were immunized intramuscularly with 1mg/construct of antigenic plasmids+/-CCL27 with electroporation. We observed significant IFN-gamma secretion and CD8+ T-cell proliferation in peripheral blood. Interestingly, CCL27 co-immunized macaques exhibited a trend toward greater effector CD4+ T cells in the bronchiolar lavage (BAL). CCL27 co-delivery also elicited greater antigen-specific IgA at unique sites including BAL and fecal samples but not in the periphery. Future studies incorporating CCL27 as an adjuvant in vaccine or therapy models where eliciting immune responses in the lung are warranted.
journal_name
Vaccinejournal_title
Vaccineauthors
Kraynyak KA,Kutzler MA,Cisper NJ,Khan AS,Draghia-Akli R,Sardesal NY,Lewis MG,Yan J,Weiner DBdoi
10.1016/j.vaccine.2009.10.095subject
Has Abstractpub_date
2010-02-23 00:00:00pages
1942-51issue
8eissn
0264-410Xissn
1873-2518pii
S0264-410X(09)01644-2journal_volume
28pub_type
杂志文章相关文献
VACCINE文献大全abstract::In recent years, collaborations often between mathematical and computational biologists and scientists in the World Health Organization (WHO) global influenza surveillance network, have resulted in a number of mathematical and computational advances including: increasing the resolution at which antigenic surveillance ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(03)00068-9
更新日期:2003-05-01 00:00:00
abstract::The development of live attenuated vaccines, allowing for the safe and effective immunisation at mucosal surfaces, is a strategy of great interest for vaccinologists. The main advantage of this approach over conventional parenteral vaccines is the induction of strong mucosal immune responses, allowing targeting of the...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2003.12.033
更新日期:2004-06-23 00:00:00
abstract::A varicella-zoster virus (VZV) seropositive individual was immunized with 100 micrograms of purified VZV TgpI-511 glycoprotein subunit antigen formulated with monophosphoryl lipid A. Serum samples were obtained during a 40-day period post-immunization (PI) and analysed by immunoprecipitation and virus neutralization t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(94)00073-v
更新日期:1995-10-01 00:00:00
abstract::Cytotoxic T cells are the main antigen-specific effector cells of the cellular immune system and MHC class I restricted cytotoxic T-lymphocyte (CTL) responses in mice, acting against the HIV-1 envelope protein, are known to be predominantly directed against an amino acid sequence in the third hypervariable domain. We ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(93)90251-r
更新日期:1993-01-01 00:00:00
abstract::Nucleic acid vaccination has many potential advantages over traditional methods, but suffers from the fact that DNA vaccines tend to be relatively poorly immunogenic. Attempts to enhance DNA vaccine immunogenicity have included the addition of cytokine-encoding plasmids into the formulation, as well as the use of hete...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.01.160
更新日期:2005-06-10 00:00:00
abstract:OBJECTIVES:We aimed to estimate vaccine effectiveness against severe measles based on the number of vaccine doses administered and the time since last vaccination. PATIENTS AND METHODS:We included measles cases aged at least 2 years and born since 1980 who were notified in France between 2006 and mid-2019. We consider...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.05.005
更新日期:2020-06-15 00:00:00
abstract::Measles, mumps, rubella and varicella are viral infections which can implicate seriously long-term sequelae of infected individuals or even the unborn child. Vaccines against the individual diseases have long been available. Global measles vaccination is estimated to have prevented more than 20million deaths during 20...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2017.07.051
更新日期:2018-08-28 00:00:00
abstract:BACKGROUND:Measles supplementary immunization activities (SIAs) are vaccination campaigns that supplement routine vaccination programs with a recommended second dose opportunity to children of different ages regardless of their previous history of measles vaccination. They are conducted every 2-4 years and over a few w...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.10.080
更新日期:2018-01-02 00:00:00
abstract::Leptospirosis is an important zoonotic disease worldwide. Subunit vaccines are an attractive intervention strategy against this disease, but potent, non-toxic adjuvants are necessary components to any effective vaccine. Among various adjuvant candidates, liposomes have garnered recent attention for their capacity as c...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.08.029
更新日期:2009-10-19 00:00:00
abstract::Influenza A viruses pose a substantial threat to the human population whether by purposeful manipulation and release or by the natural process of interspecies transmissions from animal reservoirs. The challenge with preparing for these events with vaccination strategies is that the best forms of protective immunity ta...
journal_title:Vaccine
pub_type: 杂志文章,评审
doi:10.1016/j.vaccine.2009.08.038
更新日期:2009-11-05 00:00:00
abstract::Actinobacillus pleuropneumoniae is the causative agent of porcine pleuropneumonia, a highly contagious and often fatal disease. We have previously reported the construction and characterization of a single gene apxIIC deletion mutant HB04C(-) based on A. pleuropneumoniae serovar 7 which produces ApxII toxin and ApxIV....
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.07.053
更新日期:2007-11-01 00:00:00
abstract:BACKGROUND:In January 2015, Tajikistan introduced the monovalent rotavirus vaccine into the national immunization program. Our objective was to estimate pre-vaccine burden of rotavirus-associated hospitalizations in children <5 years of age in Tajikistan. METHODS:During January 2013-December 2014, active surveillance ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.08.031
更新日期:2018-12-14 00:00:00
abstract::Classical swine fever virus (CSFV) causes significant losses in pig industry in many countries in Asia and Europe. The E2 glycoprotein of CSFV is the main target for neutralizing antibodies. Recently, the replicon of alphaviruses, such as Semliki Forest virus (SFV), has been developed as replicative expression vectors...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.08.020
更新日期:2007-04-12 00:00:00
abstract::Respiratory syncytial virus (RSV) is a major respiratory pathogen responsible for severe pulmonary disease. We have developed a parenterally administered vaccine, BBG2Na, which is currently in a phase III clinical trial. BBG2Na comprises residues 130--230 of RSV-A G protein (G2Na) fused to the BB carrier protein. In t...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/s0264-410x(01)00105-0
更新日期:2001-07-16 00:00:00
abstract::Targeting dendritic cell mannose receptors by mannosylating antigens represents a promising vaccination strategy. Using the model antigen ovalbumin (OVA) expressed recombinantly in bacterial and yeast vectors, we have previously demonstrated fungal mannosylation enhances antigen immunogenicity in the context of CD4(+)...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.03.027
更新日期:2007-05-30 00:00:00
abstract::At the Netherlands Vaccine Institute (NVI) a vaccine against Neisseria meningitidis serogroup B organisms based on different porA subtypes contained in outer membrane vesicles (OMVs) is in advanced stage of development and will be evaluated in clinical trial studies in the near future. In order to meet the expected de...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.06.008
更新日期:2007-08-21 00:00:00
abstract::Seneca Valley virus (SVV) infection in pigs is associated with porcine idiopathic vesicular disease (PIVD). Outbreaks of SVV infection in pig herds have been reported in several Asia and Americas countries. Recently, a series of outbreaks of SVV infection occurred in China, Canada, Thailand and the United States. Howe...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2017.12.055
更新日期:2018-02-01 00:00:00
abstract::The role of in vivo-induced ApxIV toxin of Actinobacillus pleuropneumoniae in protective immunity was evaluated in pigs by administering it alone or added to a multicomponent recombinant subunit vaccine composed of recombinant ApxI, ApxII, ApxIII toxin, and 42-kDa outer membrane protein (OMP). The pigs were immunized ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2009.07.065
更新日期:2009-09-25 00:00:00
abstract::As part of a larger study exploring how European citizens' balance issues of public and private interest and the extent to which they are prepared to accept State intervention on a range of public health issues, focus group participants were asked whether childhood immunisation should be a matter of parental choice or...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.11.001
更新日期:2008-01-17 00:00:00
abstract::Aging is accompanied by a decline in immune function which can lead to decreased responses to vaccines. Attenuated recombinant Vibrio cholerae O1 vaccine strain CVD 103-HgR elicits a rapid serum vibriocidal antibody (SVA) response and protects against cholera diarrhea in volunteer challenge studies but has not been st...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2019.01.077
更新日期:2019-03-07 00:00:00
abstract:PURPOSE:GEN-003 is a candidate therapeutic HSV-2 vaccine containing a fragment of infected cell protein 4 (ICP4.2), a deletion mutant of glycoprotein D2 (gD2ΔTMR), and Matrix-M2 adjuvant. In a dose-ranging phase 1/2a clinical trial, immunization with GEN-003 reduced viral shedding and the percentage of reported herpeti...
journal_title:Vaccine
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.vaccine.2016.09.001
更新日期:2016-10-17 00:00:00
abstract::M2 is the third integral membrane protein of influenza A. M2e, the extracellular, 23 amino acid residues of M2, has been remarkably conserved in all human influenza A strains. This prompted us to evaluate the use of M2e as a potential broad-spectrum immunogen in a mouse model for influenza infection. Genetic fusion of...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2006.05.082
更新日期:2006-11-10 00:00:00
abstract::In a Phase 2a trial of the RTS,S/AS vaccine, we described significant association between protection against infection and vaccine-induced CD4 T cells. To determine whether processing of the circumsporozoite protein as a component of the RTS,S particulate antigen yields the same HLA-DR-restricted epitopes as those rec...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2011.09.098
更新日期:2011-11-08 00:00:00
abstract::Although the USA is considered an area of 'low' endemicity for hepatitis B infection, the incidence of new cases, the prevalence of carriers, and the burden of acute and chronic disease place hepatitis B among the most important communicable diseases. It is estimated that 300,000 new cases of hepatitis B infection occ...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/0264-410x(95)80040-k
更新日期:1995-01-01 00:00:00
abstract::Lassa virus (LASV) is responsible for the deaths of thousands of people in West Africa annually. Genetic diversity among LASV strains is the highest among the Arenaviridae and represents a great challenge for vaccine development. Guinea pigs vaccinated with a ML29 reassortant vaccine experienced sterilizing immunity a...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2007.02.038
更新日期:2007-05-16 00:00:00
abstract::The biological activities of whole-cell pertussis vaccine and a new acellular preparation were compared. It was observed that both preparations were similarly effective in inducing protection in mice against intracerebral challenge with B. pertussis, however, the acellular preparation was approximately 200-fold more p...
journal_title:Vaccine
pub_type: 临床试验,杂志文章
doi:10.1016/0264-410x(85)90005-2
更新日期:1985-03-01 00:00:00
abstract::We recently reported an attenuated EIAV vaccine study that directly examined the effect of lentiviral envelope sequence variation on vaccine efficacy. The study [1] demonstrated for the first time the failure of an ancestral vaccine to protect and revealed a significant, inverse, linear relationship between envelope d...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2010.10.003
更新日期:2010-11-29 00:00:00
abstract::Recent findings in the interactions between pathogens and the innate immune system, particularly with dendritic cells (DC), have opened new opportunities for adjuvants designs. We have elaborated a new approach, in which gangliosides are incorporated into the outer membrane complex of Neisseria meningitidis to form ve...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2005.01.114
更新日期:2006-04-12 00:00:00
abstract:BACKGROUND:Cross-clade immunogenic stockpiled H5N1 vaccines may decrease the morbidity and transmission of infection during the initial phase of influenza pandemic. Meta-analysis of cross-reactive antibodies induced by oil-in-water emulsion adjuvanted (OWEA) influenza H5N1 virus monovalent vaccines with circulating het...
journal_title:Vaccine
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.vaccine.2017.04.029
更新日期:2017-05-31 00:00:00
abstract:BACKGROUND:Racial differences have been observed in the rate of bacterial infection and disease progression in HIV. Here, we evaluate racial differences in seasonal influenza vaccine responses. METHODS:16 healthy controls (9 Caucasians (CC) and 7 African Americans (AA)) and 26 antiretroviral therapy (ART)-treated avir...
journal_title:Vaccine
pub_type: 杂志文章
doi:10.1016/j.vaccine.2020.01.002
更新日期:2020-02-18 00:00:00