Abstract:
:Effects of thyroidectomy on Fos-like immunoreactivity (IR) in the rat brain were examined using single and double-label immunocytochemical techniques. In particular, the possibility that Fos might be involved in thyroid hormone regulation of thyrotropin releasing hormone (TRH)-containing neurons located in the parvocellular region of the paraventricular nucleus of the hypothalamus (pPVN) was examined. Adult, male, Sprague-Dawley rats were used and all animals received either surgical removal of the thyroid gland or sham surgery. Two experiments were performed. In the first experiment, animals were killed 1, 3, or 6 days after surgery and numbers of Fos-like IR cells in the parvocellular (pPVN) and magnocellular (mPVN) regions of the PVN, the anterior hypothalamic nucleus (AH), the lateral hypothalamic nucleus (LH), and the pyriform cortex were determined. In the second experiment, animals received an intracerebroventricular injection of colchicine 5 days after surgery. The next day, animals were killed and numbers of Fos-like IR cells double-labeled for either TRH, corticotropin releasing factor (CRF), or methionine-enkephalin (met-Enk) were determined. Six days after thyroidectomy there was a significant increase in the number of Fos-like IR cells detected in the pPVN. No induction in the pPVN was observed 1 and 3 days after thyroidectomy, and no effects attributable specifically to thyroidectomy (as opposed to stress) on Fos expression in the mPVN, AH, LH, or pyriform cortex were observed. In addition, a rapid, stress-related, induction of Fos-like IR was detected in the mPVN, AH, and LH and was easily distinguished from Fos expression induced in the pPVN as a function of thyroidectomy. The time course for the effect of thyroidectomy on Fos expression in the pPVN paralleled increased plasma TSH concentration. A significant correlation between numbers of Fos-like IR cells in the pPVN and plasma TSH concentration following thyroidectomy was also observed, suggesting that plasma levels of TSH correlate directly with the number of activated TRH-containing neurons located in the pPVN. Double staining for Fos and TRH, CRF, or met-Enk revealed that thyroidectomy induced Fos-like IR specifically within TRH-, but not within CRF-, or met-Enk, expressing neurons in the pPVN. Taken together, the data suggest that Fos-like IR is induced within TRH-expressing neurons in the pPVN as a consequence of decreasing levels of circulating thyroid hormone (TH). Whether this reflects a direct effect of decreasing TH on Fos expression is not yet known; however, the data are consistent with the hypothesis that Fos is involved in TH-associated regulation of TRH production and release.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Koibuchi N,Gibbs RB,Suzuki M,Pfaff DWdoi
10.1210/endo-129-6-3208subject
Has Abstractpub_date
1991-12-01 00:00:00pages
3208-16issue
6eissn
0013-7227issn
1945-7170journal_volume
129pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::We reported previously that chromogranin A (Chga) knockout (KO) mice are hypertensive and hyperadrenergic. Here we sought to determine the basis of such alterations by probing physiological, biochemical, and pharmacological responses to perturbations of the autonomic nervous system. In the conscious state, KO mice had...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0429
更新日期:2009-11-01 00:00:00
abstract::A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts as has the noncanonical behavior of IGF-...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1464
更新日期:2015-12-01 00:00:00
abstract::The primary culture of rat luteal cells and their long-term maintenance have been difficult. Low cellular yields have limited the possibility for the study of gene regulation in luteal cells. The goal of this study was to develop a cell line to serve as a model by which to study the expression and regulation of variou...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.4.5929
更新日期:1998-04-01 00:00:00
abstract::The specific uptake by rat liver of human (hGH) and bovine (bGH) GHs labeled with 125I was studied by an in vivo procedure. A significant reduction of the uptake was observed when labeled hormones were injected together with different amounts of the corresponding native GH. This reduction was dose dependent, and the c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1190
更新日期:1978-10-01 00:00:00
abstract::Estrogens play a central role in regulating female reproduction throughout the reproductive axis, and the pituitary is one of the major targets of estrogen action. We hypothesized that estrogen receptor alpha (ERalpha) mediates estrogen action in the pituitary gonadotroph. To test this hypothesis, we generated a mouse...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2007-1084
更新日期:2008-01-01 00:00:00
abstract::The ability of a monoclonal antiidiotypic antibody (clone 1D5) directed against the binding site of a monoclonal antiestradiol antibody to interact with the estrogen receptor (ER) was investigated. The following lines of evidence indicate that clone 1D5 has the capacity of mimicking the actions of estradiol, and recog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1597160
更新日期:1992-06-01 00:00:00
abstract::The hypothesis that hydrogen peroxide generation occurs in the corpora lutea of superovulated rats during luteolysis was tested using a peroxide-dependent inhibitor of catalase, 3-amino-1,2,4-triazole (AT). Luteal regression was induced during midpseudopregnancy by injection of 500 micrograms prostaglandin F2 alpha (P...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-128-4-1749
更新日期:1991-04-01 00:00:00
abstract::Peroxisome proliferator-activated receptor-gamma coactivator-1 (PGC-1), a cold-induced protein expressed in brown adipose tissue (BAT), plays a role in adaptive thermogenesis by up-regulating uncoupling proteins (UCP). Here, we explore its relationship to the thermogenic actions of leptin, which also up-regulates UCPs...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.12.7804
更新日期:2000-12-01 00:00:00
abstract::For 3 yr, 1-yr synchronized circannual rhythms were found for the chick oviduct weights, amounts of soluble protein, concentrations of the progesterone receptor in this organ, and capacity of this steroid-receptor complex in cytosol in bind in vitro to deoxyribonucleoprotein acceptor sites. By fitting the data with 1-...
journal_title:Endocrinology
pub_type: 历史文章,杂志文章
doi:10.1210/endo-107-4-1234
更新日期:1980-10-01 00:00:00
abstract::There are few data for hormonal levels and testis structure and function during postnatal development in rats neonatally treated with monosodium L-glutamate (MSG). In our study, newborn male pups were ip injected with MSG (4 mg/g body weight) every 2 d up to 10 d of age and investigated at prepubertal and adult ages. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0640
更新日期:2006-03-01 00:00:00
abstract::Since hypophysectomy and GH deficiency are associated with decreases in hypothalamic content and release of SRIF, it was of interest to determine whether these hormonal alterations also affect peripheral tissue levels of SRIF. Hypophysectomized (hypox) rats were studied at various times after surgery and compared with...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2260
更新日期:1984-12-01 00:00:00
abstract::In mammals, lactation is associated with a period of infertility characterized by the loss of pulsatile secretion of GnRH and cessation of ovulatory cycles. Despite the importance of lactational infertility in determining overall fecundity of a species, the mechanisms by which the suckling stimulus suppresses GnRH sec...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1621
更新日期:2014-01-01 00:00:00
abstract::Adrenocortical carcinoma (ACC) is an aggressive cancer with a 5-year survival rate <35%. Mortality remains high due to lack of targeted therapies. Using bioinformatic analyses, we identified maternal embryonic leucine zipper kinase (MELK) as 4.1-fold overexpressed in ACC compared with normal adrenal samples. High MELK...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00310
更新日期:2018-07-01 00:00:00
abstract::Purinergic G protein-coupled receptors (P2YR) and ion-conducting receptor-channels (P2XR) are present in the pituitary. However, their identification, expression within pituitary cell subpopulations, and the ability to elevate intracellular Ca2+ concentration ([Ca2+]i) in response to ATP stimulation were incompletely ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.11.7737
更新日期:2000-11-01 00:00:00
abstract::A soluble form of the amino-terminal extracellular (ecto-) domain of the human TSH receptor was generated. This protein was capable of binding TSH and autoimmune antibodies found in Graves' patients. A deletion mutant of the ectodomain lacking nine amino acids in the C-terminal region lost its ability to interact with...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.2.5748
更新日期:1998-02-01 00:00:00
abstract::A number of cytokines have been found to be potent regulators of bone resorption and to share the properties originally attributed to osteoclast-activating factor. One such activity, differentiation-inducing factor (DIF, D-factor) from mouse spleen cells, shares a number of biological and biochemical properties with t...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-3-1416
更新日期:1990-03-01 00:00:00
abstract::Basal hypothalamic deafferentation abolished the nycthemeral rhythm of both plasma TSH and corticosterone. ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-97-3-749
更新日期:1975-09-01 00:00:00
abstract::Stimulation of rat granulosa cell aromatase activity by FSH has recently been used as a sensitive biological end point to develop an in vitro FSH bioassay. The present report provides a detailed validation and application of this assay. In the presence of androstenedione and diethylstilbestrol, FSH stimulated estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1570
更新日期:1986-10-01 00:00:00
abstract::Diets enriched in monounsaturated fatty acids (MUFA)s have been shown to benefit glycemic control. Furthermore, MUFAs specifically stimulate secretion of the antidiabetic hormone, Glucagon-like peptide-1 (GLP-1) in vitro. To determine whether the MUFA-induced benefit in glycemic tolerance in vivo is due to increased G...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.3.8034
更新日期:2001-03-01 00:00:00
abstract::Several novel cyclic MSH analogs were synthesized, and their binding properties were tested on cells transiently expressing the human melanocortin-1 (MC1), MC3, MC4, and MC5 receptors. We discovered a novel substance (HS024) that showed about 20-fold selectivity and very high affinity (Ki = 0.29 nM) for the MC4 recept...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.12.6352
更新日期:1998-12-01 00:00:00
abstract::In order to delineate the ages of onset and decline in sensitivity to growth hormone (GH) in rats, the ability of ovine GH in vitro to stimulate amino acid uptake and protein synthesis was studied in diaphragm muscle. GH (25 mug/ml) produced a significant, but small (12-17%), stimulation of alpha-aminoisobutyric acid ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-5-1273
更新日期:1976-05-01 00:00:00
abstract::We have previously demonstrated that cultured rat thyroid cells do not exhibit constitutive expression of major histocompatibility (MHC) class II antigens. Using reovirus types 1 and 3, we infected 1B-6 cells (a cloned derivative of the Fisher rat cell line FRTL-5), and found a dose-dependent induction of thyroid cell...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-1-543
更新日期:1989-01-01 00:00:00
abstract::Aldo-keto-reductase 1B7/mouse vas deferens protein (AKR1B7/MVDP) is expressed in rodent steroidogenic glands and in the mouse vas deferens. In steroidogenic organs, AKR1B7/MVDP scavenges isocaproaldehyde produced from the cholesterol side-chain cleavage reaction. Akr1b7/mvdp is responsive to ACTH in adrenals and to an...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220825
更新日期:2003-05-01 00:00:00
abstract::The concentration of testosterone (T) and 5alpha-dihydrotestosterone (DHT) in plasma, seminal fluid, and epididymis of male Wistar rats have been determined by radioimmunoassay. The DHT/T ratio rose from 0.1 in blood to 1 in testicular fluid and 4 in epididymal fluid. This ratio is 2 in tissues of "caput" epididymis a...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-98-1-111
更新日期:1976-01-01 00:00:00
abstract::We have previously shown that in reaggregate cell cultures of 14-day-old female rat pituitary, LHRH is capable of stimulating lactotrophs to enter the S-phase of the cell cycle, and that this effect is mediated by paracrine growth factors secreted from gonadotrophs. In the present report we describe the isolation, pur...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013350
更新日期:1994-07-01 00:00:00
abstract::Serum LH and FSH were measured at 60, 30, and 0 min before, at 5, 15, and 30 min during, and at 10, 45, and 90 min after bilateral electrical stimulation (ES) of various hypothalamic regions in 12 unanesthetized ovariectomized rhesus monkeys. ES of the arcuate-ventromedial nuclei (medial basal hypothalamus; MBH) induc...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-314
更新日期:1977-02-01 00:00:00
abstract::Des-acyl ghrelin (DAG), the most abundant form of ghrelin in humans, has been found to reduce arterial blood pressure and prevent cardiac and endothelial cell apoptosis. Despite this, data regarding its direct effect on cardiac function and coronary blood flow, as well as the related involvement of autonomic nervous s...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1922
更新日期:2016-06-01 00:00:00
abstract::Estrogen has wide-ranging and complex effects on the reproductive axis, which are often difficult to interpret from in vivo studies. Estrogen negatively regulates tonic GnRH synthesis and also plays a pivotal role in the positive regulation of GnRH necessary for the preovulatory surge. To dissect the mechanisms by whi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.11.7117
更新日期:1999-11-01 00:00:00
abstract::Mutations in the GnRH receptor gene (GNRHR) can result in hypogonadotropic hypogonadism in humans. Unlike most mammals, mice lack a second form of GnRH (GnRH2) and a type 2 GnRH receptor. To determine whether the GnRH receptor is critical at all stages of reproduction and whether this receptor has additional physiolog...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2009-0598
更新日期:2010-03-01 00:00:00
abstract::The purpose of the present study was to further examine the hypothesis that activation of G protein-coupled receptor 54 (GPR54) signaling at the end of the juvenile phase of primate development is responsible for initiation of gonadarche and the onset of puberty. Accordingly, we determined whether repetitive iv admini...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-1261
更新日期:2006-02-01 00:00:00