Abstract:
BACKGROUND:Endostar is a novel recombinant human endostatin expressed and purified in Escherichia coli with the N-terminal modified. It has been shown that endostar inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) through the membrane surface receptor KDR/flt-1(VEGFR-2, vascular endothelial growth factor receptor-2) by exerting anti-angiogenesis effects. But the molecular mechanism remained unclear. MATERIALS AND METHODS:The apoptotic effects induced by endostar in the serum-deprived situation were investigated by 4'-6-diaminidino-2-phenylindole (DAPI) staining and the Annexin V-fluorescein isothiocyanate (FITC) binding assay. The mechanism of action was explored by Western blotting assay. RESULTS:Endostar induced remarkable apoptosis in HUVECs. The expressions of apoptosis-related proteins showed that caspase-3 was activated, but caspase-8, a marker of the non-mitochondria-mediated apoptosis signal pathway, was not. Further investigation revealed that cellular Bcl-2 decreased in the endostar-treated groups, while the level of Bax was almost unchanged. CONCLUSION:Endostar induces apoptotic effects in HUVECs through the activation of caspase-3 and a decrease of the Bcl-2 to Bax ratio.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Ling Y,Lu N,Gao Y,Chen Y,Wang S,Yang Y,Guo Qsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
411-7issue
1eissn
0250-7005issn
1791-7530journal_volume
29pub_type
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journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
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journal_title:Anticancer research
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journal_title:Anticancer research
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
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journal_title:Anticancer research
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doi:
更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2013-03-01 00:00:00
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journal_title:Anticancer research
pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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journal_title:Anticancer research
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journal_title:Anticancer research
pub_type: 杂志文章
doi:
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更新日期:2014-08-01 00:00:00
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journal_title:Anticancer research
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