Abstract:
BACKGROUND:Human neuroblastoma and medulloblastoma express abnormal ganglioside patterns especially GD2 and GM2 which are important for tumour growth. We tested the effects of L-cycloserine (L-CS), a potent inhibitor of synthesis of glycosphingolipids, on the growth, viability and expression of GD2 and GM2 in neuroblastoma and medulloblastoma cells. MATERIALS AND METHODS:The cytotoxic effects of L-CS were tested using the MTT dye reduction assay on four neuroblastoma (IMR-32, SK-N-SH, UKF-NB-2 and UKF-NB-3), two medulloblastoma (D283 and D341) and normal human fibroblasts and epithelial cell lines. In some experiments cytotoxicity of L-CS was tested in the presence of exogenous GD2 and GM2. The expression of GD2 and GM2 was analysed by flow cytometry. The antitumoral effects of L-CS in vivo were assessed on established xenografts of UKF-NB-3 or D283 cells in athymic (nude) mice using systemic administration of the drug (150 mg/kg intraperitoneally, once per day on 20 consecutive days). RESULTS:In vitro experiments revealed that L-CS was toxic for tumour cells at concentrations ranging from 0.5 to 20 micrograms/ml without any significant effects on normal fibroblasts and epithelial cells. L-CS treatment of UKF-NB-3 and D283 cells significantly inhibited expression of GD2 and GM2. The addition of exogenous GD2 and GM2 to culture medium partially prevented cytotoxic effects of L-CS on tumour cells. In vivo treatment resulted in complete tumour regression of UKF-NB-3 xenografts whereas growth of D283 xenografts was reduced by 60%. CONCLUSIONS:L-CS is a selective antitumoral agent for neuroblastoma and medulloblastoma cells with the ability to reduce expression of tumour associated gangliosides. In vivo experiments suggest that L-CS may be effective drug for treatment of neuroblastoma and medulloblastoma.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Cinatl J Jr,Cinatl J,Kotchetkov R,Pouckova P,Vogel JU,Rabenau H,Michaelis M,Kornhuber Bsubject
Has Abstractpub_date
1999-11-01 00:00:00pages
5349-54issue
6Beissn
0250-7005issn
1791-7530journal_volume
19pub_type
杂志文章abstract::P-glycoprotein (P-gp) is an active, ATP-dependent plasma membrane transporter which is responsible for the expulsion of various cytotoxic drugs with different chemical structures out of resistant (MDR) cells. It is also capable of transporting a number of other amphiphilic molecules, the so-called MDR-reversing agents...
journal_title:Anticancer research
pub_type: 杂志文章,评审
doi:
更新日期:1999-07-01 00:00:00
abstract::Among the limiting side-effects of anticancer drugs, hematological toxicity is the most frequent. Hematological analysis focuses on nucleated blood cells from mouse peripheral blood or hematopoietic organs (bone marrow, spleen, thymus). We have developed an automated method called A.H.E.A.D. (Automated Hematotoxicolog...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-05-01 00:00:00
abstract::We have recently reported that phagocytosis of killed Saccharomyces cerevisiae, baker's yeast, induced apoptosis in human breast cancer cell lines MCF-7, ZR-75-1 and HCC70. In this study we have evaluated the effect of treatment with MGN-3, a modified arabinoxylan from rice bran, on phagocytosis and yeast-induced apop...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND/AIM:Elderly patients represent an important subgroup of patients with brain metastases. A survival score has been developed specifically for these patients. PATIENTS AND METHODS:A total of 544 elderly patients (aged ≥65 years) receiving whole-brain radiotherapy alone were divided into a test (n=272) and a v...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-05-01 00:00:00
abstract:BACKGROUND:5-Fluoro-2'-deoxyuridine (5-FdU), a drug against gastric cancer, was covalently linked via its nucleobase with the amino-bisphosphonate alendronate (Ale), resulting in a new antimetabolite-bisphosphonate conjugate (5-FdU-Ale), designed for bone-targeting. MATERIALS AND METHODS:The cytostatic effect of 5-FdU...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND/AIM:To compare the GeneXpert® O6-methylguanine DNA methyltransferase (MGMT) methylation prototype (GX MGMT) assay with pyrosequencing in glioblastomas. MATERIALS AND METHODS:The MGMT methylation status was retrospectively assessed in formalin-fixed paraffin embedded (FFPE) tumor blocks from 262 glioblastoma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14643
更新日期:2020-11-01 00:00:00
abstract:UNLABELLED:Synthetic cannabinoid WIN55,212-2 (WIN) has shown a promise as an anticancer agent but causes psychoactive side-effects. In the present study, nano-micelles of styrene maleic acid (SMA)-conjugated WIN were synthesized to reduce side-effects and increase drug efficacy. SMA-WIN micelles were characterised and ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-09-01 00:00:00
abstract:AIM:The aim of the study was to identify novel biomarkers that are vital for improving management of patients with gastric cancer (GC). MATERIALS AND METHODS:An RNA-sequencing analysis was conducted using gastric tissue from patients with metastatic GC. In vitro cell functions were evaluated by siRNA-mediated knockdow...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13820
更新日期:2019-11-01 00:00:00
abstract::Malignant human urothelial cell lines propagated in vitro have previously been demonstrated to express low amounts of monomorphic HLA-A,B,C as compared to premalignant urothelial cells. In this study the expression of polymorphic HLA-A,B epitopes in human urothelial cell lines have been investigated in greater detail....
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract:BACKGROUND:The p16 tumour suppressor gene is known to be involved in regulation of the cell cycle. p16 expression in sequential histological stages of oral squamous cell carcinoma (OSCC) formation was investigated using an experimental model of induced oral carcinogenesis in Syrian golden hamsters. MATERIALS AND METHO...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract:BACKGROUND:We have previously shown, using the chicken embryo chorioallantoic membrane (CAM) model of in vivo angiogenesis, that X-rays act on the extracellular matrix and enhance normal and tumor-induced angiogenesis. In the present work, we studied the effect of X-rays on the gene expression of three proteins that ar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND/AIM:Malignant tumors of the salivary glands are rare and heterogeneous, with more than 20 subtypes, and classified mainly by histopathology. Their diagnosis is often challenging and their etiology unknown. Here, the possible association between human polyomaviruses (PyVs) and one or more salivary gland tumor...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12532
更新日期:2018-05-01 00:00:00
abstract:BACKGROUND/AIM:Quinazolinone is a privileged chemical structure employed for targeting various types of cancer. This study aimed to demonstrate the antitumor activity of synthesized 6,7-disubstituted-2-(3-fluorophenyl) quinazolines (HoLu-11 to HoLu-14). MATERIALS AND METHODS:The cytotoxicity was assessed by the sulfor...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14772
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND/AIM:The transcription factor Y-box-binding protein 1 (YB1) is overexpressed in many types of human cancers. YB1 regulates the G1 phase of the cell cycle by controlling transcription of G1 regulators. Here, we report that YB1 is also involved in regulating G2/M phase. MATERIALS AND METHODS:YB1-depleted TKO c...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11490
更新日期:2017-04-01 00:00:00
abstract:BACKGROUND:Estramustine is an anti-mitotic cytostatic drug that also enhances the effect of radiotherapy. The mechanism of radiosensitization is not thoroughly known. Since both radiotherapy and estramustine induce apoptosis in prostate cancer cells, we conducted an experiment to show whether radiosensitization is medi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract:BACKGROUND:Dihydroceramide desaturase 1 (DES) is the enzyme responsible for converting dihydroceramide into ceramide in the de novo sphingolipid biosynthesis pathway. Dihydroceramide can inhibit ceramide channel formation to interfere with apoptosis. We have shown that following ceramide synthase knockdown, photodynami...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::The cytotoxicity of beta-cyclodextrin benzaldehyde inclusion compound (CDBA) against human normal and cancer cell lines was investigated. CDBA showed slightly higher cytotoxicity against human tumor cell lines, as compared to normal cells, with a tumor-specificity index of 2.2. Human myelogenous leukemia cell lines (H...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract:BACKGROUND/AIM:Typical carcinoids (TC) and atypical carcinoids (AC) are rare diseases. A paucity of randomized studies and disagreements among various guidelines makes the management challenging. PATIENTS AND METHODS:Using codes for TC (8240) and AC (8249) in the National Cancer Database (NCDB), all surgically resecte...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13900
更新日期:2019-12-01 00:00:00
abstract::CA 72-4 is a high molecular weight, pancarcinoma human tumor mucin which may play an important role in the identification (i.e., staging) and clinical management of patients with gastric carcinoma. In the present study of 242 patients with primary or recurrent gastric cancer, a higher percentage of these patients had ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
abstract::Adrenocortical carcinoma (ACC) is a rare neoplasm with a poor prognosis. Prognostic factors are needed to identify patients who should be treated aggressively and those for which a less aggressive approach is warranted. As a result of advances within the field of immunohistochemistry, investigations of Ki-67, PCNA, IG...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-03-01 00:00:00
abstract::Matrix metalloproteinase-7 (MMP-7), MMP-9, MMP-13, and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) are considered to have important roles in the invasiveness and outcomes of colorectal cancer (CRC). This study examined the clinicopathological significance of the relative expression of these genes in patien...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-07-01 00:00:00
abstract:BACKGROUND/AIM:Many studies have shown an antiproliferative, anti-inflammatory, anti-angiogenetic, and apoptosis-inducing effect of Vitamin D. A vitamin D deficiency has been associated with an increased risk for different types of cancer. This study examined vitamin D 25(OH)D levels in gynaecological cancers in compar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13983
更新日期:2020-01-01 00:00:00
abstract::Long-term results of different studies employing cisplatin-based chemotherapy in advanced ovara in cancer are just beginning to be published. Available data suggest that the rate of relapse decreases but does not cease, and the question of whether cisplatin-based chemotherapy results in an improved cure in advanced ov...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章,评审
doi:
更新日期:1989-11-01 00:00:00
abstract::Medullary thyroid carcinoma (MTC), a rare calcitonin-producing tumor, is derived from parafollicular C-cells of the thyroid and is characterized by constitutive Bcl-2 overexpression. The tumor is relatively insensitive to radiation therapy as well as conventional chemotherapy. To date, the only curative treatment is t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-11-01 00:00:00
abstract::The cyanoguanidine CHS 828 was recently shown to possess potent anti-tumour effects both in vitro and in vivo. The exact mechanism of action of CHS 828 is not known, but recent results have indicated that induction of programmed cell death may be one mechanism by which CHS 828 exerts its anti-tumour effects. To invest...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Ras proteins must be isoprenylated at a conserved cysteine residue near the carboxyl terminus (Cys-186 in mammalian Ras p21 proteins) in order to extend their biological activity. Previous studies have indicate an intermediate in the mevalonate pathway, most likely farnesyl pyrophosphate, is the donor of this isopreny...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-07-01 00:00:00
abstract::Human primary breast cancers were analysed for somatic loss of heterozygosity (LOH) at chromosome 18 with 15 polymorphic microsatellite markers. LOH was observed in 148 of the 228 cases analyzed, (65%). Three smallest common deletion regions (SCDR) were detected on the long arm of chromosome 18. The marker D18S51 at t...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-03-01 00:00:00
abstract::The oxazaphosphorines cyclophosphamide (CP) and ifosfamide (IF) are alkylating agents that require bioactivation via cytochrome (CYP) P450 isoenzymes including CYP2C9 enzymes. The present study investigated CYP2C9 in regard to its allelic variants in 23 tumor samples (10 breast tumors, 1 breast tumor cell line, 5 brai...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Big Endothelin-1 levels increase significantly in patients with various tumors, and raised plasma concentrations are associated with worse outcome. The aim of this study was to investigate plasma Big Endothelin-1 levels in patients with gastric carcinoma before and after radical gastrectomy and to explore it...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-05-01 00:00:00
abstract::Therapy of patients with metastatic renal cell carcinoma (mRCC) requires sequential use of several agents with different mechanisms and minimal cross-resistance between the different agents. Tyrosine kinase inhibitors (TKIs) and mammalian target of rapamycin (mTOR) inhibitors prolong progression-free survival (PFS) in...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-10-01 00:00:00