Micro-CT analysis of alveolar bone healing using a rat experimental model of critical-size defects.

Abstract:

OBJECTIVE:This study was designed to establish a rat model of a critical size alveolar bone defect. MATERIALS AND METHODS:Standardized buccal or mesiobuccal alveolar bone defects were made around the right first mandibular molar of 12-week-old rats, and the left was used as a control. Alveolar bone healing was examined quantitatively by three-dimensional micro-computed tomographic imaging. Bone matrix production of osteoblasts and osteocytes during repair of alveolar bone defects was examined with in situ hybridization for type I collagen. RESULTS:Buccal defects were repaired significantly and the volume decreased by 88.3% in week 24, whereas mesiobuccal defects were repaired little. Osteoblasts and osteocytes expressed type I collagen in both defects in week 3 but showed little expression by week 6 and thereafter, leaving the mesiobuccal defects largely unrepaired. CONCLUSION:The mesiobuccal defect is a critical-size defect that is not ultimately repaired with bone. It may be an appropriate experimental model for investigating the effectiveness of bone regenerative agents in human alveolar bone loss.

journal_name

Oral Dis

journal_title

Oral diseases

authors

Ebina H,Hatakeyama J,Onodera M,Honma T,Kamakura S,Shimauchi H,Sasano Y

doi

10.1111/j.1601-0825.2009.01522.x

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

273-80

issue

4

eissn

1354-523X

issn

1601-0825

pii

ODI1522

journal_volume

15

pub_type

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