Abstract：
:Trypanosoma congolense epimastigote forms (EMFs) adhere to the tsetse fly proboscis, proliferate, and differentiate into animal-infective metacyclic forms (MCFs). This differentiation step, called metacyclogenesis, is indispensable for the cyclical transmission of the parasite. Although an in vitro metacyclogenesis culture system was established several decades ago, few genetic tools have been utilized to investigate the molecular mechanisms underlying T. congolense metacyclogenesis. This study established a transgene expression system using an in vitro derived EMF of T. congolense IL3000, and the transgenic EMF successfully underwent metacyclogenesis in vitro. The newly constructed expression vector pSAK was designed for integration into the alpha-beta tubulin locus, which is tandemly arranged in the T. congolense genome. The expression cassette of pSAK/enhanced green fluorescent protein (eGFP) was transfected into the EMF by electroporation. An EMF expressing eGFP was successfully generated and differentiated into an MCF that constitutively expressed eGFP. The in vitro metacyclogenesis system in combination with the transgenic EMF technique will be important tools to investigate the molecular mechanisms of metacyclogenesis.
journal_name
Parasitol Intjournal_title
Parasitology internationalauthors
Sakurai T,Tanaka M,Kawazu S,Inoue Ndoi
10.1016/j.parint.2008.12.001subject
Has Abstractpub_date
2009-03-01 00:00:00pages
110-3issue
1eissn
1383-5769issn
1873-0329pii
S1383-5769(08)00122-0journal_volume
58pub_type
杂志文章abstract：:The association between liver fluke infection caused by Opisthorchis viverrini and cholangiocarcinoma (CCA - hepatic cancer of the bile duct epithelium) has been well established. Multiple mechanisms play a role in the development of CCA, but the excretory/secretory products released by O. viverrini (OvES) represent t...
journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2016.02.001
更新日期：2017-08-01 00:00:00
abstract：:Muscular sarcocystosis by Sarcocystis arctica was found for the first time in the Czech Republic, in different muscles of red fox (Vulpes vulpes). Cysts were slim, elongated, thread-like, whitish, 1-7mm long, and 206-270μm wide; bradyzoites were 7.9×2.7μm in unstained wet mounts and 9.2×2.9μm in cyst Giemsa-stained sm...
journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2017.05.003
更新日期：2017-10-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2018.03.006
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abstract：:In Shiqu County of the Qinghai-Tibet plateau, many wild and domestic mammals are involved in the transmission cycles of Echinococcus spp. Echinococcus multilocularis and Echinococcus granulosus genotype G1 (sheep strain) are sympatrically distributed in the plateau. In 1995, we identified a unique strobilate stage of ...
journal_title：Parasitology international
pub_type： 杂志文章,评审
doi：10.1016/j.parint.2005.11.035
更新日期：2006-01-01 00:00:00
abstract：:Trichinella sp. muscle larvae were isolated from the thigh muscle of two red foxes (Vulpes vulpes) captured in Sapporo and Otofuke, Hokkaido, Japan, in 2003. Multiplex PCR designed for genotyping the genus Trichinella revealed that the Sapporo isolate showed a specific pattern to T. britovi complex (T. britovi, Trichi...
journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2006.08.004
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2017.11.002
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2012.06.004
更新日期：2012-12-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2012.10.006
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journal_title：Parasitology international
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journal_title：Parasitology international
pub_type： 杂志文章
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2013.07.011
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2020.102200
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journal_title：Parasitology international
pub_type： 杂志文章
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更新日期：2000-01-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/s1383-5769(01)00108-8
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2007.09.006
更新日期：2008-06-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章,评审
doi：10.1016/j.parint.2009.02.001
更新日期：2009-06-01 00:00:00
abstract：:Taeniasis/cysticercosis has been reported from several provinces of Indonesia: Papua (=former Irian Jaya), Bali, North Sumatra, East Nusa Tenggara, South East Sulawesi, Lampung, North Sulawesi, Jakarta, West Kalimantan, and East Java. The highest level of endemicity of taeniasis/cysticercosis has been found in Papua. ...
journal_title：Parasitology international
pub_type： 杂志文章,评审
doi：10.1016/j.parint.2005.11.025
更新日期：2006-01-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2017.04.001
更新日期：2017-10-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章,评审
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更新日期：2017-08-01 00:00:00
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journal_title：Parasitology international
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doi：10.1016/j.parint.2016.02.011
更新日期：2016-10-01 00:00:00
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journal_title：Parasitology international
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journal_title：Parasitology international
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journal_title：Parasitology international
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journal_title：Parasitology international
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journal_title：Parasitology international
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journal_title：Parasitology international
pub_type： 杂志文章
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更新日期：1999-03-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
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更新日期：2013-12-01 00:00:00
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journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2015.03.003
更新日期：2015-10-01 00:00:00
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journal_title：Parasitology international
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doi：10.1016/j.parint.2006.06.006
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abstract：:Diphyllobothrium hottai Yazaki, Fukumoto & Abe, 1988 was described based on the morphology of adult worms recovered from golden hamsters that had been experimentally infected with plerocercoids obtained from Japanese surf smelts (Hypomesus pretiosus japonicus) and olive rainbow smelts (Osmerus eperlanus mordax). Altho...
journal_title：Parasitology international
pub_type： 杂志文章
doi：10.1016/j.parint.2016.06.011
更新日期：2016-10-01 00:00:00
