Abstract:
:We describe the cloning of a full length calpain-encoding cDNA constructed from two truncated cDNAs isolated from a cDNA library prepared with mRNA isolated from adult worms of the Philippine strain of Schistosoma japonicum. The cDNA sequence is 2.456 kb in length and predicts a protein of 758 residues with a molecular mass of 86.61 kDa and an isoelectric point of 5.34. Probes spanning the entire calpain cDNA hybridised to multiple bands in genomic DNAs of Philippine (SjP) and Chinese (SjC) S. japonicum, with some restriction fragment length polymorphisms evident between the two strains. Northern hybridisation analysis indicated that the cDNA codes for a single RNA transcript between 2.6 and 3.6 kb in size in the SjP and SjC genomes. After subcloning in the QIA express vectors pQE-31 and pQE-40 and subsequent expression, the recombinant protein was purified and shown to bind calcium. The availability of recombinant S. japonicum calpain will allow its future evaluation as a vaccine candidate, especially in light of recent work with the S. mansoni homologue which has provided evidence that this protein may be a target of protective immunity.
journal_name
Parasitol Intjournal_title
Parasitology internationalauthors
Scott JC,McManus DPdoi
10.1016/s1383-5769(99)00021-5subject
Has Abstractpub_date
2000-01-01 00:00:00pages
205-14issue
3eissn
1383-5769issn
1873-0329pii
S1383-5769(99)00021-5journal_volume
48pub_type
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journal_title:Parasitology international
pub_type: 杂志文章,评审
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journal_title:Parasitology international
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journal_title:Parasitology international
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journal_title:Parasitology international
pub_type: 杂志文章
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更新日期:2000-02-01 00:00:00
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journal_title:Parasitology international
pub_type: 杂志文章,评审
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更新日期:2009-06-01 00:00:00
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journal_title:Parasitology international
pub_type: 杂志文章
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更新日期:2018-10-01 00:00:00
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pub_type: 杂志文章
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journal_title:Parasitology international
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journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2014.08.006
更新日期:2015-02-01 00:00:00
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pub_type: 杂志文章
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journal_title:Parasitology international
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Parasitology international
pub_type: 杂志文章
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journal_title:Parasitology international
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journal_title:Parasitology international
pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00
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journal_title:Parasitology international
pub_type: 杂志文章
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journal_title:Parasitology international
pub_type: 杂志文章,评审
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更新日期:2003-12-01 00:00:00
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journal_title:Parasitology international
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-04-01 00:00:00
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journal_title:Parasitology international
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journal_title:Parasitology international
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更新日期:2013-02-01 00:00:00
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journal_title:Parasitology international
pub_type: 杂志文章
doi:10.1016/j.parint.2019.02.004
更新日期:2019-06-01 00:00:00
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journal_title:Parasitology international
pub_type: 杂志文章
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journal_title:Parasitology international
pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00