Abstract：
:We present evidence demonstrating that genes encoding enzymes essential for successful blood-feeding are differentially induced in the midgut of the hard tick Haemaphysalis longicornis. Three serine proteinase genes (HlSP, HlSP2 and HlSP3) isolated from H. longicornis midgut exhibit protein sequence similarity with other trypsin-like serine proteinases reported from arthropods and vertebrate animal species. The endogenous enzymes were mainly detected in the midgut epithelial cells and in the lumen of an adult tick. The recombinant enzymes expressed in Escherichia coli efficiently hydrolyzed synthetic substrates specific for serine proteinases over a broad range of pH and temperature values. Notably, the transcript levels of HlSP2 and HlSP3 were detected to significantly increase at 96 h post infestation, while the transcript of HlSP was induced in the earlier stage of blood-feeding. Further, silencing of HlSP, HlSP2 and HlSP3 genes by RNA interference led to a significant reductions in the engorged tick body weight, suggesting synergetic roles of these serine proteinases in blood-feeding and digestion.
journal_name
Parasitol Intjournal_title
Parasitology internationalauthors
Miyoshi T,Tsuji N,Islam MK,Alim MA,Hatta T,Huang X,Fujisaki Kdoi
10.1016/j.parint.2008.08.003subject
Has Abstractpub_date
2008-12-01 00:00:00pages
499-505issue
4eissn
1383-5769issn
1873-0329pii
S1383-5769(08)00093-7journal_volume
57pub_type
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