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Evaluation of the effect of protease inhibitors on the viability of Miamiensis avidus using the WST-1 assay.

Abstract：

:Miamiensis avidus causes scuticociliatosis in cultured olive flounders (Paralichthys olivaceus), leading to economic losses in aquaculture in Korea. Quantitative evaluation of the viability of M. avidus is important to develop an effective vaccine or chemotherapeutic agent against it. We used a colorimetric assay based on the reduction of 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1) to quantify the viability of M. avidus. Using this method, we investigated the effect of protease inhibitors on the viability of M. avidus. The assay showed a clear difference in the optical density (OD) of over 104 ciliates, and the metalloprotease inhibitors 1, 10-phenanthroline and ethylenediaminetetraacetic acid (EDTA) reduced the viability of M. avidus by more than 90% when used at concentration of 5 mM and 100 μM, respectively. However, different morphological changes in the parasite were observed when exposed to these two inhibitors. These results indicate that the WST-1 assay is a simple and reliable method to quantify the viability of M. avidus, and metalloproteases are excellent targets for the development of agents and vaccines to control M. avidus infection.

journal_name

Parasitol Int

journal_title

Parasitology international

authors

Shin SP,Sohn HC,Jin CN,Lee J

doi

10.1016/j.parint.2020.102093

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

102093

eissn

1383-5769

issn

1873-0329

pii

S1383-5769(20)30043-X

journal_volume

76

pub_type

杂志文章

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