Evidence that the signal-initiating membrane protein CD9 is associated with small GTP-binding proteins.

Abstract:

:F(ab')2 fragments of anti-CD9 mAb aggregate platelets by a thromboxane-dependent pathway implicating CD9 as signal initiating molecule. We demonstrate that mAbs directed against CD9, but not against GPIIb/IIIa specifically immunoprecipitate, from detergent lysates of human platelets, proteins of 25 and 26 kDa which bind [alpha 32P]GTP on nitrocellulose transfers. The binding is specific since it is blocked by GTP, but not by ATP. The GTP-binding proteins do not belong to a Mg(2+)-sensitive subset since they are unaffected by the addition of 2 microM-20 mM Mg2+. The observations demonstrate that CD9 is associated with selected small G-proteins.

authors

Seehafer JG,Shaw AR

doi

10.1016/0006-291x(91)91384-o

subject

Has Abstract

pub_date

1991-08-30 00:00:00

pages

401-6

issue

1

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)91384-O

journal_volume

179

pub_type

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