Abstract：
:In the present study, we questioned whether the cholesterol synthesis inhibitor lovastatin potentiates the cytotoxicity of chemotherapeutic agents in the primitive CD34(+) subpopulation of acute myeloid leukemia (AML) cells. AML mononuclear cells (n = 17) were sorted in CD34(+) and CD34(-) fractions and compared to normal CD34(+/-) cells (n = 7). The percentage of surviving cells upon exposure to lovastatin (25-100 microM) and/or chemotherapeutics (cytarabin or daunorubicin) was determined with a luminescent cell viability assay. The results demonstrate that the primitive CD34(+) subpopulation of normal and AML cells displayed a higher sensitivity to lovastatin than the more mature CD34(-) subpopulation. The combination of lovastatin and chemotherapeutics resulted in a more pronounced inhibitory effect on both subpopulations. In contrast to the homogeneous results in normal CD34(+) cells, a distinct heterogeneity in lovastatin sensitivity was found in AML samples. Therefore, a group of normal (n = 11) and abnormal (n = 6) responders were identified based on a reduced or increased cell survival compared to normal CD34(+) cells. This distinction was not only observed in the CD34(+) AML subfraction but also in CD34(+)CD38(-)AML cells. In the abnormal responder group, 50% of patients presented with unfavorable cytogenetics and significant higher peripheral blast cell counts, which coincided with poor treatment results. In summary, the findings indicate that the primitive subfraction of CD34(+) AML cells is in the majority of cases affected by lovastatin treatment, which is potentiated when combined with chemotherapeutics. Heterogeneity of the response observed in AML patients allowed identification of a subgroup with poor prognosis.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
de Jonge-Peeters SD,van der Weide K,Kuipers F,Sluiter WJ,de Vries EG,Vellenga Edoi
10.1007/s00277-008-0633-2subject
Has Abstractpub_date
2009-06-01 00:00:00pages
573-80issue
6eissn
0939-5555issn
1432-0584journal_volume
88pub_type
杂志文章abstract：:B cell acute lymphoblastic leukemia (B-ALL) is an aggressive hematologic malignancy with limited treatment strategies. Histone deacetylases inhibitors (HDACis) are promising novel tools for cancer therapy, whose anti-tumor effects and the underlying mechanisms on B-ALL remain to be elucidated. Recently, Notch1 signali...
journal_title：Annals of hematology
pub_type： 杂志文章
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更新日期：2013-01-01 00:00:00
abstract：:Among 13,525 haemoglobin analyses performed in our laboratory we detected 21 cases of haemoglobin D (Hb D) disease. Investigation of a family affected with this abnormal haemoglobin revealed two cases of Hb D/beta-(0) thalassaemia for the first time among Saudi Arabs. The two patients were diagnosed as having chronic ...
journal_title：Annals of hematology
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abstract：:Thrombotic thrombocytopenia purpura (TTP) and atypical hemolytic uremic syndromes (aHUS) are distinct clinical disorders characterized by hemolytic anemia, thrombocytopenia, microthrombi, and end organ damage. TTP is characterized by a low ADAMTS13 activity level at diagnosis of <10 % ADAMTS13 activity, while aHUS is ...
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pub_type： 临床试验,杂志文章
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更新日期：2015-09-01 00:00:00
abstract：:Cerebrovascular disease resulting in stroke is a serious and preventable complication of sickle cell anaemia (SCA). Children at high risk of preventable stroke can be identified by transcranial Doppler ultrasound (TCD). Current guidelines in the UK recommend annual TCD screening from 3 years, although studies suggest ...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-009-0757-z
更新日期：2009-10-01 00:00:00
abstract：:Isocitrate dehydrogenase IDH 1 and IDH 2 mutations were reported in several cancer forms, especially in hematological malignancies, but were never been investigated in familial aggregation. The aim of this study is to determine whether germline isocitrate dehydrogenase genes mutations are involved.We targeted IDH1 and...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-016-2813-9
更新日期：2016-12-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770100288
更新日期：2001-05-01 00:00:00
abstract：:Bortezomib is one of the most widely used novel drugs for the treatment of multiple myeloma (MM). However, twice-weekly intravenous administration is associated with innegligible adverse events and treatment discontinuation. We therefore evaluated the long-term efficacy and feasibility of reduced frequency treatment w...
journal_title：Annals of hematology
pub_type： 杂志文章,多中心研究
doi：10.1007/s00277-016-2661-7
更新日期：2016-05-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-006-0107-3
更新日期：2006-07-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-005-1068-7
更新日期：2005-10-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-016-2913-6
更新日期：2017-04-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-015-2570-1
更新日期：2016-02-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF01715351
更新日期：1992-02-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-003-0716-z
更新日期：2003-10-01 00:00:00
abstract：:Extramedullary (e) relapse in multiple myeloma(MM) has an adverse prognosis, but knowledge concerning biological features and preferred treatment is scarce. We screened the myeloma registry of our institution for eMM relapses and identified 24 cases among 357 patients (pts).Only 8% of eMM relapses occurred after initi...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-012-1414-5
更新日期：2012-07-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-003-0792-0
更新日期：2004-06-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-017-3211-7
更新日期：2018-03-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s00277-004-0850-2
更新日期：2004-01-01 00:00:00
abstract：:There is increasing evidence for the role of chronic antigenic stimulation (CS) in the development of cancer. Clinical data, however, are rare as is the information on outcome. In this study, the occurrence of chronic infections (CI) and autoimmune diseases (AI) in patients with malignant lymphoma at diagnosis was ass...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-011-1178-3
更新日期：2011-08-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF01715386
更新日期：1995-01-01 00:00:00
abstract：:Early cytomegalovirus (CMV) replication (eCMV) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) has been suggested as an independent factor that reduces leukemia relapse risk. We retrospectively analyzed 74 patients with acute myeloid leukemia (AML) who underwent allo-HSCT between August 2006 and S...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-014-2190-1
更新日期：2015-02-01 00:00:00
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journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF00641311
更新日期：1996-02-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/BF01698483
更新日期：1994-08-01 00:00:00
abstract：:Although B chronic lymphocytic leukemia (B-CLL) cells express the alpha chain of the interleukin-2 (IL-2) receptor CD25, little is known about the effect of IL-2 on apoptosis in B-CLL cells. We have shown previously that stimulation of B-CLL cells with a CpG-oligonucleotide induces IL-2 high affinity receptors. In our...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-010-0994-1
更新日期：2010-11-01 00:00:00
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journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770050518
更新日期：1999-07-01 00:00:00
abstract：:Multiple myeloma (MM) is an incurable disease accompanied by low plasma levels of low-density lipoprotein cholesterol (LDL-c). The significance of altered cholesterol metabolism in the pathophysiology of MM remains elusive. Although it has been hypothesized that myeloma cells depend on exogenous cholesterol for its su...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-011-1246-8
更新日期：2012-01-01 00:00:00
abstract：:Lower dosage of total body irradiation (TBI) and chemotherapy in reduced-intensity conditioning (RIC) regimens prior to allogeneic stem cell transplantation have reduced the toxicity of the conditioning and non-relapse mortality. The FLAMSA-RIC protocol for high-risk patients with acute myeloid leukemia (AML) and myel...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s00277-011-1253-9
更新日期：2012-01-01 00:00:00
abstract：:Since December 2019, a novel coronavirus has spread throughout China and across the world, causing a continuous increase in confirmed cases within a short period of time. Some studies reported cases of thrombocytopenia, but hardly any studies mentioned how the virus causes thrombocytopenia. We propose several mechanis...
journal_title：Annals of hematology
pub_type： 杂志文章,评审
doi：10.1007/s00277-020-04019-0
更新日期：2020-06-01 00:00:00
abstract：:Pharmacological agents such as hydroxyurea (HU) have been known to cause induction of fetal hemoglobin and possibly may alleviate the symptoms in thalassemia intermedia patients. Thirty-seven patients with beta-thalassemia intermedia were enrolled to assess response to HU therapy. Major response was defined as transfu...
journal_title：Annals of hematology
pub_type： 临床试验,杂志文章
doi：10.1007/s00277-005-1026-4
更新日期：2005-07-01 00:00:00
abstract：:In multiple myeloma, the polymerase chain reaction (PCR) of the Ig heavy chain with allele-specific oligonucleotide (ASO) primers is a common and well-described method of identifying the tumor clone in peripheral blood (PB), bone marrow (BM) or leukapheresis products (LA). A factor which is crucial to the detection of...
journal_title：Annals of hematology
pub_type： 杂志文章
doi：10.1007/s002770000245
更新日期：2001-02-01 00:00:00
