Variability in responsiveness to lovastatin of the primitive CD34+ AML subfraction compared to normal CD34+ cells.


:In the present study, we questioned whether the cholesterol synthesis inhibitor lovastatin potentiates the cytotoxicity of chemotherapeutic agents in the primitive CD34(+) subpopulation of acute myeloid leukemia (AML) cells. AML mononuclear cells (n = 17) were sorted in CD34(+) and CD34(-) fractions and compared to normal CD34(+/-) cells (n = 7). The percentage of surviving cells upon exposure to lovastatin (25-100 microM) and/or chemotherapeutics (cytarabin or daunorubicin) was determined with a luminescent cell viability assay. The results demonstrate that the primitive CD34(+) subpopulation of normal and AML cells displayed a higher sensitivity to lovastatin than the more mature CD34(-) subpopulation. The combination of lovastatin and chemotherapeutics resulted in a more pronounced inhibitory effect on both subpopulations. In contrast to the homogeneous results in normal CD34(+) cells, a distinct heterogeneity in lovastatin sensitivity was found in AML samples. Therefore, a group of normal (n = 11) and abnormal (n = 6) responders were identified based on a reduced or increased cell survival compared to normal CD34(+) cells. This distinction was not only observed in the CD34(+) AML subfraction but also in CD34(+)CD38(-)AML cells. In the abnormal responder group, 50% of patients presented with unfavorable cytogenetics and significant higher peripheral blast cell counts, which coincided with poor treatment results. In summary, the findings indicate that the primitive subfraction of CD34(+) AML cells is in the majority of cases affected by lovastatin treatment, which is potentiated when combined with chemotherapeutics. Heterogeneity of the response observed in AML patients allowed identification of a subgroup with poor prognosis.


Ann Hematol


Annals of hematology


de Jonge-Peeters SD,van der Weide K,Kuipers F,Sluiter WJ,de Vries EG,Vellenga E




Has Abstract


2009-06-01 00:00:00












  • Impact of extramedullary plasmacytomas on outcomes according to treatment approach in newly diagnosed symptomatic multiple myeloma.

    abstract::The prognostic impact of extramedullary plasmacytomas (EMPs) on newly diagnosed symptomatic multiple myeloma (MM) was evaluated in the context of treatment approach including autologous stem cell transplantation (ASCT) and chemotherapy alone. A total of 275 consecutive patients with newly diagnosed MM were included, a...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Lee SE,Kim JH,Jeon YW,Yoon JH,Shin SH,Eom KS,Kim YJ,Kim HJ,Lee S,Cho SG,Lee JW,Min WS,Park CW,Min CK

    更新日期:2015-03-01 00:00:00

  • Residual methylation of tumor suppressor gene promoters, RASSF6 and RASSF10, as novel biomarkers for minimal residual disease detection in adult acute lymphoblastic leukemia.

    abstract::Aberrant promoter methylation of RASSF6 and RASSF10 occurs at a high frequency in acute lymphoblastic leukemia (ALL). Because of the complexity of the current minimal residual disease (MRD) detecting-methods, the DNA methylation status of the RASSF6 and RASSF10 genes could potentially become biomarkers for the assessm...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Younesian S,Shahkarami S,Ghaffari P,Alizadeh S,Mehrasa R,Ghaffari SH

    更新日期:2019-12-01 00:00:00

  • Clinical heterogeneity of silent-gene b-thalassemia among Indians.

    abstract::This paper describes six Indian families in which silent-gene beta-thalassemia was encountered by us in the past 2 years. This stresses the importance of globin-chain synthesis in the diagnosis of beta-thalassemia. Of these six cases, five were diagnosed retrospectively when they had a homozygous beta-thalassemia offs...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Nadkarni A,Pawar A,Mudera VC,Mohanty D,Colah R

    更新日期:1995-01-01 00:00:00

  • Blood B, T, CD4+ and CD8+ lymphocytes in female Wistar rats.

    abstract::We have established reference values of peripheral blood lymphocyte subsets in healthy female Wistar rats under highly standardized conditions. Using monoclonal antibodies and flow cytometry, T lymphocytes (OX19+), B lymphocytes (OX6+ and anti-Ig+), T-helper/inducer (W3/25+), and T-suppressor/cytotoxic subsets (OX8+) ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Franch A,Castellote C,Pelegrí C,Tolosa E,Castell M

    更新日期:1993-09-01 00:00:00

  • A comparison of qPCR and ddPCR used for quantification of the JAK2 V617F allele burden in Ph negative MPNs.

    abstract::Philadelphia-negative myeloproliferative neoplasms (MPNs) are a diverse group of diseases whose common feature is the presence of V617F mutation of the JAK2 gene. In the era of novel therapeutic strategies in MPNs, such as JAK-inhibitor therapy, there is a growing need for establishing high sensitive quantitative meth...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Link-Lenczowska D,Pallisgaard N,Cordua S,Zawada M,Czekalska S,Krochmalczyk D,Kanduła Z,Sacha T

    更新日期:2018-12-01 00:00:00

  • Risk for cytomegalovirus infection following reduced intensity allogeneic stem cell transplantation.

    abstract::Preliminary data suggest a faster immune recovery following non-myeloablative stem cell transplantation because of the persistence of recipient T cells, but the real impact on post-transplant infectious complications remains unknown. We retrospectively analysed the incidence of cytomegalovirus (CMV) infection in twent...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Nachbaur D,Larcher C,Kircher B,Eibl G,Nussbaumer W,Gunsilius E,Haun M,Grünewald K,Gastl G

    更新日期:2003-10-01 00:00:00

  • Bloodstream infections with gram-negative organisms and the impact of multidrug resistance in patients with hematological malignancies.

    abstract::Infections and especially blood stream infections (BSI) with gram-negative bacteria (GNB) represent a major threat for patients with hematological diseases undergoing chemotherapy and mainly contribute to morbidity and mortality. In this retrospective single-center study, we analyzed the impact of BSI with different g...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Scheich S,Weber S,Reinheimer C,Wichelhaus TA,Hogardt M,Kempf VAJ,Kessel J,Serve H,Steffen B

    更新日期:2018-11-01 00:00:00

  • Cytomegalovirus infection in seropositive unrelated cord blood recipients: a study of 349 Korean patients.

    abstract::To gain insight into the natural history of cytomegalovirus (CMV) infection following unrelated cord blood transplantation (UCBT) in seropositive patients, we analyzed the data of 349 seropositive patients who received UCBT in Korea between 2000 and 2011. CMV reactivation occurred in 49 % (171/349) of the CMV-seroposi...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Park M,Lee YH,Lee SH,Yoo KH,Sung KW,Koo HH,Lee JW,Kang HJ,Park KD,Shin HY,Ahn HS,Lee JW,Chung NG,Cho B,Kim HK,Koh KN,Im HJ,Seo JJ,Baek HJ,Kook H,Hwang TJ,Lee JM,Hah JO,Lim YJ,Park JE,Lyu CJ,Lim YT,Ch

    更新日期:2015-03-01 00:00:00

  • Antibiotic prevention of pneumococcal infections in asplenic hosts: admission of insufficiency.

    abstract::Asplenic and hyposplenic patients have an increased risk for overwhelming pneumococcal infections, even several decades after splenectomy. Pneumococcal vaccination and daily oral administration of penicillin V are recommended to prevent such infections, 2-5 years after splenectomy, and for at least 5 years in children...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: de Montalembert M,Lenoir G

    更新日期:2004-01-01 00:00:00

  • Rituximab plus bendamustine is active in pretreated patients with extragastric marginal zone B cell lymphoma of the mucosa-associated lymphoid tissue (MALT lymphoma).

    abstract::Recently, the combination of rituximab and bendamustine (R-Benda) has been defined as highly active in patients with follicular lymphomas, but little is known about the efficacy of R-Benda in mucosa-associated lymphoid tissue (MALT) lymphoma. In a retrospective analysis, we have defined 14 patients with MALT lymphoma ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Kiesewetter B,Mayerhoefer ME,Lukas J,Zielinski CC,Müllauer L,Raderer M

    更新日期:2014-02-01 00:00:00

  • Morphologic and phenotypic changes of the leukemic cells in a case of marginal zone B-cell lymphoma.

    abstract::A particular case of marginal zone B-cell lymphoma (MZBCL) presenting with leukemic lymphocytes is reported. In the present observation, the leukemic cells not only displayed a remarkable morphological fluctuation but also had an unusual phenotype, changing with time. These phenotypic features, which have been functio...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Dargent JL,Delville JP,Kornreich A,Pradier O,Cochaux P,Velu T,Capel P,Feremans W,Neve P

    更新日期:1997-03-01 00:00:00

  • Acute myeloid/NK precursor cell leukemia with trisomy 4 and a novel point mutation in the extracellular domain of the G-CSF receptor in a patient with chronic idiopathic neutropenia.

    abstract::Chronic idiopathic neutropenia (CIN) has been well recognized as a granulocytic disorder not associated with increased risk to malignant transformation. Four cases, however, of acute myeloid leukemia have been recently reported in patients with CIN. In the current paper, we report on a CIN patient who developed acute ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Papadaki HA,Kosteas T,Gemetzi C,Damianaki A,Anagnou NP,Eliopoulos GD

    更新日期:2004-06-01 00:00:00

  • The predictive value of selected serum microRNAs for acute GVHD by TaqMan MicroRNA arrays.

    abstract::Currently, the diagnosis of acute graft-versus-host disease (aGVHD) is mainly based on clinical symptoms and biopsy results. This study was designed to further explore new no noninvasive biomarkers for aGVHD prediction/diagnosis. We profiled miRNAs in serum pools from patients with aGVHD (grades II-IV) (n = 9) and non...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Zhang C,Bai N,Huang W,Zhang P,Luo Y,Men S,Wen T,Tong H,Wang S,Tian YP

    更新日期:2016-10-01 00:00:00

  • Statin use and the risk of multiple myeloma: a PRISMA-compliant meta-analysis.

    abstract::Previous studies exploring associations between statin use and risk of multiple myeloma (MM) showed inconsistent results. We searched for articles published in English in databases (PubMed, Web of Science, EMBASE, Medline, and Google Scholar) before October 2019. The multivariate odds ratio (OR)/relative risk (RR) and...

    journal_title:Annals of hematology

    pub_type: 杂志文章,meta分析


    authors: Zhang P,Liu B

    更新日期:2020-08-01 00:00:00

  • A novel NAP1L4/NUTM1 fusion arising from translocation t(11;15)(p15;q12) in a myeloid neoplasm with eosinophilia and rearrangement of PDGFRA highlights an unusual clinical feature and therapeutic reaction.

    abstract::NUT midline carcinoma (NMC) is an aggressive neoplasm and mainly involved in the head and neck area. The defining genetic hallmark on these tumors is that testis-specific nuclear gene (NUTM1) fuses to bromodomain protein family member 4 gene (BRD4), resulting in the formation of BRD4-NUTM1 transcript. Here, we report ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Cheng Z,Luo Y,Zhang Y,Wang Y,Chen Y,Xu Y,Peng H,Zhang G

    更新日期:2020-07-01 00:00:00

  • In vitro megakaryocyte expansion in patients with delayed platelet engraftment after autologous stem cell transplantation.

    abstract::Increasing the number of megakaryocytic cells in stem cell transplants by ex vivo expansion culture may provide an approach to accelerate platelet engraftment after high-dose chemotherapy. However, it is unknown if a relationship exists between the expansion potential of progenitor cells and the time to platelet engra...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Drayer AL,Smit Sibinga CT,Esselink MT,de Wolf JT,Vellenga E

    更新日期:2002-04-01 00:00:00

  • Intermediate intensity conditioning regimen containing FLAMSA, treosulfan, cyclophosphamide, and ATG for allogeneic stem cell transplantation in elderly patients with relapsed or high-risk acute myeloid leukemia.

    abstract::Lower dosage of total body irradiation (TBI) and chemotherapy in reduced-intensity conditioning (RIC) regimens prior to allogeneic stem cell transplantation have reduced the toxicity of the conditioning and non-relapse mortality. The FLAMSA-RIC protocol for high-risk patients with acute myeloid leukemia (AML) and myel...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Chemnitz JM,von Lilienfeld-Toal M,Holtick U,Theurich S,Shimabukuro-Vornhagen A,Krause A,Brossart P,Hallek M,Scheid C

    更新日期:2012-01-01 00:00:00

  • The Hellenic type of nondeletional hereditary persistence of fetal hemoglobin results from a novel mutation (g.-109G>T) in the HBG2 gene promoter.

    abstract::Nondeletional hereditary persistence of fetal hemoglobin (nd-HPFH), a rare hereditary condition resulting in elevated levels of fetal hemoglobin (Hb F) in adults, is associated with promoter mutations in the human fetal globin (HBG1 and HBG2) genes. In this paper, we report a novel type of nd-HPFH due to a HBG2 gene p...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Chassanidis C,Kalamaras A,Phylactides M,Pourfarzad F,Likousi S,Maroulis V,Papadakis MN,Vamvakopoulos NK,Aleporou-Marinou V,Patrinos GP,Kollia P

    更新日期:2009-06-01 00:00:00

  • Genetic research and clinical analysis of deletional Chinese Gγ+(Aγδβ)0 -thalassemia and Southeast Asian HPFH in South China.

    abstract::Chinese Gγ+(Aγδβ)0-thalassemia and SEA-HPFH are the most common types of β-globin gene cluster deletion in Chinese population. The aim of the study was to analyze clinical features of deletional Chinese Gγ+(Aγδβ)0-thalassemia and Southeast Asian hereditary persistence of fetal hemoglobin (SEA-HPFH) in South China. A t...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Wu Y,Yao Q,Zhong M,Wu J,Xie L,Su L,Yu F

    更新日期:2020-12-01 00:00:00

  • No evidence for neoantigens in human plasma after photochemical virus inactivation.

    abstract::Photodynamic virus inactivation of human fresh plasma mediated by visible light in the presence of the phenothiazine dyes methylene blue or toluidine blue was investigated to determine whether it influences functional, structural, and immunological properties of plasma proteins. The activities of the coagulation facto...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Mohr H,Knüver-Hopf J,Lambrecht B,Scheidecker H,Schmitt H

    更新日期:1992-11-01 00:00:00

  • Radiotherapy and PGEMOX/GELOX regimen improved prognosis in elderly patients with early-stage extranodal NK/T-cell lymphoma.

    abstract::The optimal treatment strategy for elderly patients with natural killer/T-cell lymphoma (NKTCL) remains to be established. A total of 63 elderly patients with newly diagnosed NKTCL were retrospectively reviewed. Among the patients with stage I-II disease, 58.3 % received radiotherapy (RT) ± chemotherapy, and 41.7 % re...

    journal_title:Annals of hematology

    pub_type: 临床试验,杂志文章


    authors: Bi XW,Xia Y,Zhang WW,Sun P,Liu PP,Wang Y,Huang JJ,Jiang WQ,Li ZM

    更新日期:2015-09-01 00:00:00

  • L-tryptophan-related eosinophilia-myalgia syndrome possibly associated with a chronic B-lymphocytic leukemia.

    abstract::A female patient presenting with B-CLL and coincident eosinophilia-myalgia syndrome (EMS) after ingestion of L-tryptophan is described. The manifestations of EMS disappeared completely during treatment with cyclophosphamide/prednisone. and there was an intermittent clinical remission of CLL with absence of the monoclo...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Böhme A,Wolter M,Hoelzer D

    更新日期:1998-11-01 00:00:00

  • The effect of pre-eclampsia on complete blood count, platelet count and mean platelet volume.

    abstract::Pre-eclampsia is a condition observed during pregnancy and threatens the life of both mother and foetus. There are studies, which suggest platelets play a major role in the pathogenesis of pre-eclampsia. The aim of this study is to compare the complete blood count (CBC) parameters, especially platelet count and mean p...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Ceyhan T,Beyan C,Başer I,Kaptan K,Güngör S,Ifran A

    更新日期:2006-05-01 00:00:00

  • Increased cell apoptosis in bone marrow trephine biopsies and immunomagnetically isolated myeloid progenitor cells in patients with chronic idiopathic neutropenia.

    abstract::The frequency of apoptotic cells in bone marrow trephine biopsies and cytospins of immunomagnetically isolated myeloid progenitor cells was determined in 39 patients with chronic idiopathic neutropenia (CIN) and 12 hematologically normal individuals using the in situ end-labeling (ISEL) apoptosis detection method. We ...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Koumaki V,Papadaki HA,Stefanaki K,Damianaki A,Gemetzi C,Katonis P,Vrentzos G,Eliopoulos GD

    更新日期:2003-10-01 00:00:00

  • Exacerbation of antiphospholipid antibody syndrome after treatment of localized cancer: a report of two cases.

    abstract::Patients with malignancy often present with a variety of coagulation abnormalities which may ultimately lead to recurrent arterial and venous thromboses. Recently the presence of antiphospholipid antibodies in cancer patients has been proposed as one of the potential mechanisms promoting hypercoagulability. Here we re...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Langer F,Eifrig B,Marx G,Stork A,Hegewisch-Becker S,Hossfeld DK

    更新日期:2002-12-01 00:00:00

  • Plasma concentrations of total/free and functional protein S are not decreased in systemic lupus erythematosus patients with lupus anticoagulant and/or antiphospholipid antibodies.

    abstract::We conducted an investigation to clarify whether or not the levels of total, free, and functional protein S and C4-binding protein (C4bp) in plasma are decreased in systemic lupus erythematosus (SLE) patients, especially those with antiphospholipid antibody (aPL), which is known to be a causative factor of such compli...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Matsuda J,Gohchi K,Gotoh M,Tsukamoto M,Saitoh N

    更新日期:1994-12-01 00:00:00

  • The association of venous thromboembolism with survival in pediatric cancer patients: a population-based cohort study.

    abstract::Venous thromboembolism (VTE) is a well-recognized complication in pediatric oncology patients. Studies in adult oncology patients have suggested a potential negative association between VTE and survival, but this association has not been examined in pediatric patients yet. The aim of this study was to assess the assoc...

    journal_title:Annals of hematology

    pub_type: 杂志文章,多中心研究


    authors: Forbrigger Z,Kuhle S,Brown MM,Moorehead PC,Digout C,Kulkarni K

    更新日期:2018-10-01 00:00:00

  • Flow cytometric assay of phosphotyrosine levels in Bcr-Abl-positive chronic myelogenous leukemias: a potential prognostic marker.

    abstract::Assay of phosphotyrosine levels using flow cytometry has been used to identify patients with chronic myelogenous leukemia positive for the Bcr-Abl fusion gene. We hypothesized that clinical monitoring could identify treatment response through reductions in intragranulocyte phosphotyrosine. Initially, we studied cell l...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Sun X,Li J,Chen J,Li D,Chen L,Xu W,Yang Y,Wu Y,Jiang P,Xie W

    更新日期:2009-01-01 00:00:00

  • Predictors of clinical responses to hypomethylating agents in acute myeloid leukemia or myelodysplastic syndromes.

    abstract::Azacitidine and decitabine, two hypomethylating agents, are known to be effective in the treatment of high-risk myelodysplastic syndromes (MDS) and acute myeloid leukemia (AML) patients who cannot endure intensive cytotoxic chemotherapy or are not eligible for transplantation. However, the treatment response rate is l...

    journal_title:Annals of hematology

    pub_type: 杂志文章,评审


    authors: Wang H,Li Y,Lv N,Li Y,Wang L,Yu L

    更新日期:2018-11-01 00:00:00

  • Lymphoma-like monoclonal B cell lymphocytosis in a patient population: biology, natural evolution, and differences from CLL-like clones.

    abstract::High-count monoclonal B cell lymphocytosis (MBL) with a chronic lymphocytic leukemia (CLL) phenotype is a well-known entity, featuring 1-4% annual risk of progression towards CLL requiring treatment. Lymphoma-like MBL (L-MBL), on the other hand, remains poorly defined and data regarding outcome are lacking. We retrosp...

    journal_title:Annals of hematology

    pub_type: 杂志文章


    authors: Vander Meeren S,Heyrman B,Renmans W,Bakkus M,Maes B,De Raeve H,Schots R,Jochmans K

    更新日期:2018-07-01 00:00:00