Abstract:
:In multiple myeloma, the polymerase chain reaction (PCR) of the Ig heavy chain with allele-specific oligonucleotide (ASO) primers is a common and well-described method of identifying the tumor clone in peripheral blood (PB), bone marrow (BM) or leukapheresis products (LA). A factor which is crucial to the detection of clonal Ig rearrangements lies in the 'purity' of the tumor tissue used for the consensus PCR. We describe the application of a method to enrich CD138 positive myeloma cells derived from weakly infiltrated PB-, BM- and LA-samples. These are subjected to immunomagnetic enrichment with the MACS system, using an CD138 antibody directly conjugated to magnetic beads to obtain an enriched tumor cell population and the subsequent amplification of tumor specific IgH rearrangements. We investigated 29 samples (ten PB, ten BM, nine LA) with a median myeloma cell content of 0.5%. The approach led to a median enrichment factor of 118. Tumor-specific rearrangements could be amplified reproducibly from samples containing less than 0.1% myeloma cells.
journal_name
Ann Hematoljournal_title
Annals of hematologyauthors
Draube A,Pfister R,Vockerodt M,Schuster S,Kube D,Diehl V,Tesch Hdoi
10.1007/s002770000245subject
Has Abstractpub_date
2001-02-01 00:00:00pages
83-9issue
2eissn
0939-5555issn
1432-0584journal_volume
80pub_type
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journal_title:Annals of hematology
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journal_title:Annals of hematology
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journal_title:Annals of hematology
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更新日期:2001-09-01 00:00:00
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journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
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pub_type: 杂志文章,评审
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更新日期:2020-03-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s002770100351
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journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-008-0633-2
更新日期:2009-06-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
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更新日期:2016-06-01 00:00:00
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journal_title:Annals of hematology
pub_type: 临床试验,杂志文章
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abstract::Polo-like kinase 2 (SNK/PLK2), a transcriptional target for wild-type p53 and is hypermethylated in a high percentage of multiple myeloma and B cell lymphomas patients. Given these data, we sought to study the methylation status of the specific gene in acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS), a...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-011-1193-4
更新日期:2011-09-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章
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更新日期:2001-06-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章,多中心研究
doi:10.1007/s00277-006-0110-8
更新日期:2006-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00277-013-1981-0
更新日期:2014-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s00277-020-04252-7
更新日期:2020-12-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/BF01703239
更新日期:1993-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-09-01 00:00:00
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journal_title:Annals of hematology
pub_type: 杂志文章
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abstract::Recent studies have demonstrated that direct cell-to-cell interaction is one of the microenvironment factors for transdifferentiation of adult stem cells into cardiomyocytes. We investigated whether transdifferentiation of mesenchymal stem cells (MSCs) into cardiomyocytes was dependent on developmental stages of cocul...
journal_title:Annals of hematology
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abstract::Early cytomegalovirus (CMV) replication (eCMV) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) has been suggested as an independent factor that reduces leukemia relapse risk. We retrospectively analyzed 74 patients with acute myeloid leukemia (AML) who underwent allo-HSCT between August 2006 and S...
journal_title:Annals of hematology
pub_type: 杂志文章
doi:10.1007/s00277-014-2190-1
更新日期:2015-02-01 00:00:00
abstract::We evaluated the clinical features, treatment modalities, treatment responses, and prognosis of our patients with immune thrombocytopenia (ITP). Furthermore, we estimated the frequency of ITP in the Thrace region of Turkey. Two hundred sixteen patients diagnosed with ITP between 2000 and 2012 at our center were retros...
journal_title:Annals of hematology
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abstract::Preliminary data suggest a faster immune recovery following non-myeloablative stem cell transplantation because of the persistence of recipient T cells, but the real impact on post-transplant infectious complications remains unknown. We retrospectively analysed the incidence of cytomegalovirus (CMV) infection in twent...
journal_title:Annals of hematology
pub_type: 杂志文章
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journal_title:Annals of hematology
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abstract::Infections and especially blood stream infections (BSI) with gram-negative bacteria (GNB) represent a major threat for patients with hematological diseases undergoing chemotherapy and mainly contribute to morbidity and mortality. In this retrospective single-center study, we analyzed the impact of BSI with different g...
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journal_title:Annals of hematology
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00