Expression of alkaline sphingomyelinase in yeast cells and anti-inflammatory effects of the expressed enzyme in a rat colitis model.


:Alkaline sphingomyelinase (Alk-SMase) is a key enzyme in the intestinal tract for digestion of dietary sphingomyelin (SM), which generates lipid messengers with cell-cycle regulating effects. The enzyme is significantly decreased in ulcerative colitis and colon cancer. Based on this information, we wanted to investigate whether the enzyme had preventive effects against murine colitis. We report herein a method to express a biologically active Alk-SMase from Pichia pastoris yeast cells. By using the expressed enzyme to treat a rat colitis model induced by dextran sulfate sodium, we found that intrarectal instillation of Alk-SMase once daily for 1 week significantly reduced the inflammation score and protected the colonic epithelium from inflammatory destruction. We found a tendency for decreased tumor necrosis factor (TNF)-alpha expression in the Alk-SMase-treated group. This study, for the first time, provides a method to produce the enzyme and shows the potential applicability of the enzyme in the treatment of inflammatory bowel diseases.


Dig Dis Sci


Andersson D,Kotarsky K,Wu J,Agace W,Duan RD




Has Abstract


2009-07-01 00:00:00












  • Diffuse excess mucosal collagen in rectal biopsies facilitates differential diagnosis of solitary rectal ulcer syndrome from other inflammatory bowel diseases.

    abstract::Solitary rectal ulcer syndrome (SRUS) is sufficiently uncommon that the clinician or general pathologist may lack familiarity with the disorder and may confuse it with other inflammatory bowel diseases. To evaluate the role of collagen staining in facilitating the differential diagnosis of SRUS, an initial open review...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Levine DS,Surawicz CM,Ajer TN,Dean PJ,Rubin CE

    更新日期:1988-11-01 00:00:00

  • Post-sphincterotomy transampullary balloon dilation is a safe and effective technique.

    abstract:PURPOSE:To evaluate the safety and efficacy of performing ampullary balloon dilation (ABD) following endoscopic sphincterotomy (ES). METHODS:Retrospective review of patients that underwent ABD at Thomas Jefferson University from 2000 to 2007. In all cases, dilation was performed with Hurricane RX hydrostatic balloons ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Kowalski T,Nathwani RA,Assis D,Salese L,Banwait K,Loren D

    更新日期:2009-03-01 00:00:00

  • Curative Criteria After Endoscopic Resection for Superficial Esophageal Squamous Cell Carcinomas.

    abstract:BACKGROUND:According to the Japanese Esophageal Society (JES) guidelines, risk factors for lymph node (LN) metastasis in the muscularis mucosa (MM)/submucosa to a depth of up to 200 μm (SM1) in cases of esophageal squamous cell carcinomas (ESCCs) include the presence of lymphatic invasion (ly), venous invasion (v), inf...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Mizumoto T,Hiyama T,Oka S,Yorita N,Kuroki K,Kurihara M,Yoshifuku Y,Sanomura Y,Urabe Y,Murakami Y,Arihiro K,Tanaka S,Chayama K

    更新日期:2018-06-01 00:00:00

  • Breath-ammonia testing of healthy subjects and patients with cirrhosis.

    abstract:BACKGROUND:Hepatic encephalopathy (HE) is a serious neuropsychiatric complication in both acute and chronic liver disease. AIMS:To establish the utility of a portable noninvasive method to measure ammonia in the breath of healthy subjects and patients with HE. METHODS:The study included 106 subjects: 44 women and 62 ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Adrover R,Cocozzella D,Ridruejo E,García A,Rome J,Podestá JJ

    更新日期:2012-01-01 00:00:00

  • Examination of tissue distribution of Helicobacter pylori within columnar-lined esophagus.

    abstract::H. pylori may colonize columnar-lined esophagus, although an etiologic role in esophageal adenocarcinoma is unproven. H. pylori can adhere to intestinal metaplasia in the stomach. This study was designed to examine if H. pylori adheres to specialized intestinal metaplasia in columnar-lined esophagus. Esophageal biopsi...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Sharma VK,Demian SE,Taillon D,Vasudeva R,Howden CW

    更新日期:1999-06-01 00:00:00

  • Pancreatitis as initial presentation of cystic fibrosis in young adults. A report of two cases.

    abstract::Because of marked improvement in diagnosis and management, cystic fibrosis is now more frequently noted in young adults, many of whom have only mild clinical forms. Pancreatitis is a known complication of cystic fibrosis and may occur at any time in the course of the disease; in rare instances, pancreatitis is the fir...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Masaryk TJ,Achkar E

    更新日期:1983-10-01 00:00:00

  • Adalimumab for Induction of Histological Remission in Moderately to Severely Active Ulcerative Colitis.

    abstract:BACKGROUND:Histological remission represents a target distinct from endoscopic healing in ulcerative colitis (UC) and seems a better predictor of clinical outcomes. AIMS:The aim of this study was to assess the ability of adalimumab to achieve histological remission in UC patients. METHODS:Single-center, retrospective...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Fernández-Blanco JI,Fernández-Díaz G,Cara C,Vera MI,Olivares D,Taxonera C

    更新日期:2018-03-01 00:00:00

  • Gastrointestinal and Hepatic Manifestations of Ebola Virus Infection.

    abstract:AIM:Although Ebola virus infection (EVI) clinically presents with common, prominent, gastroenterologic manifestations, this subject has not been previously reviewed. This work critically and comprehensively reviews this subject. METHODS:This study is a comprehensive literature review generated by computerized search o...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章,评审


    authors: Sharma N,Cappell MS

    更新日期:2015-09-01 00:00:00

  • Colectomy with Permanent End Ileostomy Is More Cost-Effective than Ileal Pouch-Anal Anastomosis for Crohn's Colitis.

    abstract:BACKGROUND:Much of the economic burden of Crohn's disease (CD) is related to surgery. Twenty percent of patients with CD have isolated colonic disease. While permanent end ileostomy (EI) is generally the procedure of choice for patients with refractory CD colitis, single-center experiences suggest that restorative proc...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Taleban S,Van Oijen MG,Vasiliauskas EA,Fleshner PR,Shen B,Ippoliti AF,Targan SR,Melmed GY

    更新日期:2016-02-01 00:00:00

  • Development of an Algorithm to Identify Cases of Nonalcoholic Steatohepatitis Cirrhosis in the Electronic Health Record.

    abstract:BACKGROUND AND AIMS:Current genetic research of nonalcoholic steatohepatitis (NASH) cirrhosis is limited by our ability to accurately identify cases on a large scale. Our objective was to develop and validate an electronic health record (EHR) algorithm to accurately identify cases of NASH cirrhosis in the EHR. METHODS...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Danford CJ,Lee JY,Strohbehn IA,Corey KE,Lai M

    更新日期:2020-06-13 00:00:00

  • Targeted Physician Education and Standardizing Documentation Improves Documented Reporting with Inflammatory Bowel Disease Quality Measures in a Large Academic and Private Practice.

    abstract:BACKGROUND:Prior studies have shown poor compliance with quality measures for IBD at academic and private practices. We sought to provide focused interventions to improve compliance and documentation with the IBD measures. METHODS:Two centers, academic practice (AP) and private practice (PP), initially reviewed their ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Feuerstein JD,Papamichael K,Popejoy S,Nadelson A,Lewandowski JJ,Geissler K,Martinez-Vazquez M,Leffler DA,Ariyabuddhiphongs K,Thukral C,Cheifetz AS

    更新日期:2018-01-01 00:00:00

  • One stomach--one strain: does Helicobacter pylori strain variation influence disease outcome?

    abstract::The aim of the study was to determine inter- and intrapatient variation of Helicobacter pylori strains based on genomic fingerprinting and cagA (cytotoxin-associated gene A) status. Ten bacterial colonies from each of 10 patients with gastric cancer (GC), 10 with duodenal ulcer (DU), and 10 with gastritis (GI) were us...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Enroth H,Nyrén O,Engstrand L

    更新日期:1999-01-01 00:00:00

  • Sera DNA Methylation of CDH1, DNMT3b and ESR1 Promoters as Biomarker for the Early Diagnosis of Hepatitis B Virus-Related Hepatocellular Carcinoma.

    abstract:BACKGROUND:DNA methylation mainly affects tumor suppressor genes in the development of hepatocellular carcinoma (HCC). However, sera methylation of specific genes in hepatitis B virus (HBV)-related HCC remains unknown. AIMS:The purpose of this study was to identify methylation frequencies of sera E-cadherin (CDH1), DN...

    journal_title:Digestive diseases and sciences

    pub_type: 临床试验,杂志文章


    authors: Dou CY,Fan YC,Cao CJ,Yang Y,Wang K

    更新日期:2016-04-01 00:00:00

  • Modafinil in the treatment of debilitating fatigue in primary biliary cirrhosis: a clinical experience.

    abstract::Modafinil may be a potentially effective treatment for primary biliary cirrhosis (PBC)-related fatigue. About 42 patients were given a 3-day trial of 100-200 mg modafinil. Response was defined as increased energy, decreased somnolence and sleep requirements, and improved daily function. Patients with positive response...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Ian Gan S,de Jongh M,Kaplan MM

    更新日期:2009-10-01 00:00:00

  • Diagnostic value of "bread-barium" swallow in patients with esophageal symptoms.

    abstract::The value of a "bread-barium" swallow for diagnosis of disordered esophageal motility was examined in 53 patients, 20 of whom complained of dysphagia, 20 retrosternal chest pain, and 13 heartburn; a further 19 subjects served as controls. Esophageal manometry in the patients with dysphagia and chest pain revealed majo...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Davies HA,Evans KT,Butler F,McKirdy H,Williams GT,Rhodes J

    更新日期:1983-12-01 00:00:00

  • Salmonella colitis presenting as a segmental colitis resembling Crohn's disease.

    abstract::Salmonella species have recently been shown to involve the colon in a diffuse fashion resembling ulcerative colitis by radiographic and endoscopic appearance. We describe a case of Salmonella colitis in which the radiographic and colonoscopic features were focal ulcerations, mucosal edema, friability, and a loss of no...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Vender RJ,Marignani P

    更新日期:1983-09-01 00:00:00

  • Neurofibromatosis with gastrointestinal stromal tumors: insights into the association.

    abstract::The frequent association of stromal tumors with neurofobromatosis raises high suspicion of a possible correlation between the two entities. The aim of this study was to analyze clinicopathologic features of patients with concomitant neurofibromatosis and gastrointestinal stromal tumors and to discuss the molecular bas...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Cheng SP,Huang MJ,Yang TL,Tzen CY,Liu CL,Liu TP,Hsiao SC

    更新日期:2004-08-01 00:00:00

  • Effects of aspirin and prostaglandin E2 on interdigestive motility complex and duodenogastric reflux in man.

    abstract::Both increased duodenogastric reflux and chronic aspirin ingestion are associated with the development of gastric ulcers in man. Animal studies suggest aspirin increases duodenogastric reflux. Prostaglandin E2 protects gastric mucosa from the effects of many injurious agents and inhibits gastric motility, but its effe...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Dooley CP,Mello WD,Valenzuela JE

    更新日期:1985-06-01 00:00:00

  • Treatment of genotype 4 hepatitis C recurring after liver transplantation using a combination of pegylated interferon alfa-2a and ribavirin.

    abstract:BACKGROUND:Hepatitis C virus (HCV) recurrence after liver transplantation (LT) is universal and tends to be more aggressive. Data on post-transplant HCV genotype 4 treatment is scarce. The aim of this study is to assess the safety and efficacy of pegylated interferon alpha-2a (PEG-IFN) in combination with ribavirin in ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Al-Hamoudi W,Mohamed H,Abaalkhail F,Kamel Y,Al-Masri N,Allam N,Alqahtani S,Al-Sofayan M,Khalaf H,Al-Sebayel M,Al-Jedai A,Abdo A

    更新日期:2011-06-01 00:00:00

  • Correction to: Antenatal Management for Women with Inflammatory Bowel Disease: Experience from Our 'IBD MOM' Clinic.

    abstract::The original version of the article unfortunately contained tagging error in first and family name of authors Ariella Bar-Gil Shitrit and Ami Ben Ya'acov. This has been corrected with this erratum. ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章,已发布勘误


    authors: Bar-Gil Shitrit A,Cohen Y,Hassin O,Ben Ya'acov A,Farkash R,Koslowsky B,Milgrom Y,Livovsky DM,Samueloff A,Goldin E,Grisaru-Granovsky S

    更新日期:2018-09-01 00:00:00

  • Effects of intestinal electrical stimulation on intestinal dysrhythmia and symptoms in dogs.

    abstract::The aim of this study was to investigate the effect of intestinal electrical stimulation on small intestinal dysrhythmia and motion sickness-like symptoms induced by vasopressin. Female dogs chronically implanted with two pairs of electrodes on jejunum serosa were used in a four-session study. Saline and vasopressin w...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Liu J,Wang L,Chen JD

    更新日期:2004-05-01 00:00:00

  • Artificial liver. State of the art.

    abstract::If an effective hepatic assist system existed, it could serve as a bridge to transplantation. Most of the patients waiting for liver transplantation have chronic liver insufficiency but are not in hepatic coma. Various hepatic assist systems have been used to salvage patients with acute liver insufficiency. Most attem...

    journal_title:Digestive diseases and sciences

    pub_type: 历史文章,杂志文章,评审


    authors: Takahashi T,Malchesky PS,Nosé Y

    更新日期:1991-09-01 00:00:00

  • Intrahepatic biliary cystadenoma. Clinical, radiological, and pathological findings.

    abstract::A 24-year-old white woman presented with abdominal discomfort associated with a firm, nonpulsatile hepatic mass. Radiological examination, which included radionuclide liver scan, abdominal ultrasonography, computed tomography, and hepatic angiography, demonstrated a large, globular, multilocular cyst arising from the ...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Marcial MA,Hauser SC,Cibas ES,Braver J

    更新日期:1986-08-01 00:00:00

  • Clinical significance of discordant positive hepatitis C virus transcription-mediated amplification following end of treatment response.

    abstract:BACKGROUND:Polymerase chain reaction (PCR) assays are the standard for detecting hepatitis C virus viremia. The transcription-mediated amplification (TMA)-based assay is more sensitive. METHODS:We retrospectively reviewed the charts of patients with a post-end of treatment (EOT) PCR-/TMA+ result to assess the clinical...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Thadani A,Harley J,Rubin J,Lebovics E

    更新日期:2012-01-01 00:00:00

  • Suboptimal Bowel Preparation Significantly Impairs Colonoscopic Detection of Non-polypoid Colorectal Neoplasms.

    abstract:BACKGROUND:It is unclear whether the quality of bowel preparation affects colonoscopic detection of non-polypoid colorectal neoplasms (NP-CRNs). AIM:To evaluate the impact of bowel-cleansing quality on detection of NP-CRNs. METHODS:We performed a retrospective analysis of asymptomatic screening colonoscopy cases afte...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Oh CH,Lee CK,Kim JW,Shim JJ,Jang JY

    更新日期:2015-08-01 00:00:00

  • Effect of aspirin on gallbladder motility in patients with gallstone disease. A randomized, double-blind, placebo-controlled trial of two dosage schedules.

    abstract::Patients with gallstone disease have impaired gallbladder motility. Prostaglandins are thought to be important mediators of gallbladder hypomotility. We assessed the effect of aspirin, a prostaglandin inhibitor on gallbladder resting volume and ejection fraction according to a double-blind study protocol in 20 healthy...

    journal_title:Digestive diseases and sciences

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Das A,Baijal SS,Saraswat VA

    更新日期:1995-08-01 00:00:00

  • Regional variations in neurokinin receptor subtype contributions to muscularis mucosae and epithelial function in rat colon.

    abstract::It is known that the muscularis mucosae and mucosa are not pharmacologically homogeneous throughout the rat colon. The aim of this study was to simultaneously characterize all three neurokinin (NK) receptors in the muscularis mucosae and mucosa along the length of the rat colon. Strips of proximal, mid, and distal col...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Appleyard CB,Morales M,Percy WH

    更新日期:2006-03-01 00:00:00

  • Factors affecting the success of endoscopic bougia dilatation of radiation-induced esophageal stricture.

    abstract:AIMS:The purpose of this retrospective study was to assess clinical outcomes of endoscopic bougie dilation of esophageal strictures after radiation therapy for head and neck cancer, and to assess the risk factors which affect the treatment success. METHODS:Thirty-one patients with esophageal stricture due to radiation...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Tuna Y,Koçak E,Dinçer D,Köklü S

    更新日期:2012-02-01 00:00:00

  • Hepatopulmonary syndrome in noncirrhotic portal hypertensive patients.

    abstract::Hepatopulmonary syndrome has yet not been sufficiently assessed in noncirrhotic portal hypertension. The prevalence of hepatopulmonary syndrome was determined in 31 consecutive patients with noncirrhotic portal hypertension (19 idiopathic portal hypertension, 7 portal vein thrombosis, 5 congenital hepatic fibrosis) an...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Kaymakoglu S,Kahraman T,Kudat H,Demir K,Cakaloglu Y,Adalet I,Dincer D,Besisik F,Boztas G,Sözen AB,Mungan Z,Okten A

    更新日期:2003-03-01 00:00:00

  • The human intestinal microflora.

    abstract::The major host defense mechanisms against bacterial overgrowth in the small bowel are the normal propulsive activity of the bowel itself and gastric acid secretion. Microbial interactions are a major factor in regulating the indigenous bacterial flora. Studies of the bacterial enzymes of the gut suggest that changes i...

    journal_title:Digestive diseases and sciences

    pub_type: 杂志文章


    authors: Simon GL,Gorbach SL

    更新日期:1986-09-01 00:00:00