Expression of a chimeric CSF1R-LTK mediates ligand-dependent neurite outgrowth.

Abstract:

:In an earlier screening, we identified several genes for kinases that might control the extension of neurites. One of these genes encoded a leukocyte tyrosine kinase (LTK), which is a receptor tyrosine kinase whose ligands remain to be identified. To examine the possible role of this LTK in neurite outgrowth, we constructed a chimeric receptor, in which the extracellular domain of the receptor for colony-stimulating factor-1 was fused to the cytoplasmic domain of LTK, which allowed the selective activation of LTK by colony-stimulating factor-1. Our studies using this chimeric receptor suggest that activation of the tyrosine kinase activity of LTK is sufficient to promote neurite outgrowth through pathways that include reactions catalyzed by phosphatidylinositol 3-kinase and MAPK.

journal_name

Neuroreport

journal_title

Neuroreport

authors

Yamada S,Nomura T,Takano K,Fujita S,Miyake M,Miyake J

doi

10.1097/WNR.0b013e3283186bf8

subject

Has Abstract

pub_date

2008-11-19 00:00:00

pages

1733-8

issue

17

eissn

0959-4965

issn

1473-558X

journal_volume

19

pub_type

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